411 research outputs found
Closely related and yet special – how SPβ family phages control lysis–lysogeny decisions
No full texthttp://dx.doi.org/10.13039/100015931 Max-Buchner-Forschungsstiftunghttp://dx.doi.org/10.13039/501100001659 German Research Foundatio
SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo
No full textThe detection and analysis of protein-protein interactions is one of the central tasks of proteomics in the postgenomic era. For this purpose, we present a procedure, the Strep-protein interaction experiment (SPINE) that combines the advantages of the Strep-tag protein purification system with those of reversible in vivo protein crosslinking by formaldehyde. Using two Bacillus subtilis regulator proteins, we demonstrate that this method is well suited to isolate protein complexes with high purity and virtually no background. Plasmids allowing the high-level expression of proteins carrying an N- or C-terminal Strep-tag in B. subtilis were constructed
INTELLEKTUELLE UND SCHULE
No full textOvaj se rad bavi ambivalentnim odnosom intelektualaca prema
instituciji škole i obrnuto. Na temelju specifi čnih samoopisa intelektualaca koji se mogu sažeti kroz pojmove istine, razuma i humanosti autor smatra da bi institucija škole trebala uložiti veći napor kako bi pridobila intelektualce kao partnere u dijalogu i kao savjetnike u institucionalnom i pedagoškom razvojnom
procesu. Preporuka je upućena i drugoj strani, odnosno intelektualcima, koji bi se (više) trebali pozabaviti temama škole i školske pedagogije, kao što je to bio slučaj sedamdesetih godina dvadesetog stoljeća. Autor razvija suprotstavljene tipove centripetalnih i centrifugalnih intelektualaca i predstavlja značajne intelektualce čije je djelovanje bilo od dalekosežnog pedagoškog značaja na međunarodnoj razini. Na kraju postaje jasno da je stalno kretanje od unutarnjeg ka vanjskom, od inkluzije prema ekskluziji i natrag, ono što intelektualce predodređuje da budu pedagoški inovativni. Ovo je vidljivo na primjerima školskih projekata
„Glocksee“ i „Laborschule Bielefeld“, koje je nemoguće zamisliti bez njihovih inicijatora i intelektualnih savjetnika Oskara Negta i Hartmuta von Hentiga.This paper addresses the ambivalent relationship between intellectuals and the institution of school. Based on the specific self-descriptions of intellectuals which can be summarized through the concepts of truth, reason and humanity, the author calls on the institution of school to make an increased effort into winning intellectuals as partners in dialogue and advisors for the institutional and pedagogical developmental process. On the other hand, intellectuals are encouraged to take a (more) active role in the topics related to school and school pedagogy, as was the case in the 70ies of the 20th century. The author develops the oppositeness of centripetal and centrifugal types of intellectuals and presents important intellectuals whose work had far-reaching benefi ts for education on an international level. Finally, it is clear that that the side-to-side pendulum motion between the inside and the outside and between inclusion and exclusion is what predetermines intellectuals to innovate. The examples of the two school projects - Glocksee and Laborschule Bielefeld – shows that they would be hard to imagine
without their initiators and scientific advisors Oskar Negt and Hartmund von Hentig.In dem Aufsatz geht es um das ambivalente Verhältnis von Intellektuellen zur Schule und vice versa. Basierend auf den spezifi schen Selbstbeschreibungen von Intellektuellen, die sich mit den Begriffen Wahrheit, Vernunft und Humanität überschreiben lassen, wird dafür plädiert, dass sich Schule verstärkt darum bemüht, Intellektuelle als Dialogpartner und Ratgeber für den institutionellen und pädagogischen Entwicklungsprozess zu gewinnen. Aber auch umgekehrt wird die Empfehlung artikuliert, dass sich Intellektuelle
(wieder) stärker mit Schule und schulpädagogischen Themen auseinandersetzen, wie das in den siebziger Jahren des zwanzigsten Jahrhunderts einmal der Fall war. Das Gegenüber von zentripetalen und zentrifugalen Intellektuellen-Typen wird entwickelt. Bedeutende Intellektuelle werden vorgestellt, deren Wirken international von weitreichender pädagogischer Bedeutung war. Letztlich wird deutlich, dass es das Pendeln zwischen Drinnen und Draußen, zwischen Inklusion und Exklusion ist, was den Intellektuellen prädestiniert,
pädagogisch innovativ zu sein. Exemplarisch kann das an den beiden
Schulprojekten „Glocksee“ und „Laborschule Bielefeld“ entfaltet werden, die ohne ihre Initiatoren und intellektuellen Begleiter Oskar Negt und Hartmut von Hentig kaum denkbar sind
Calibration and performance of the photon sensor response of FACT - the first G-APD Cherenkov telescope
No full textThe First G-APD Cherenkov Telescope (FACT) is the first in-operation test of the performance of silicon photo detectors in Cherenkov Astronomy. For more than two years it is operated on La Palma, Canary Islands (Spain), for the purpose of long-term monitoring of astrophysical sources. For this, the performance of the photo detectors is crucial and therefore has been studied in great detail. Special care has been taken for their temperature and voltage dependence implementing a correction method to keep their properties stable. Several measurements have been carried out to monitor the performance. The measurements and their results are shown, demonstrating the stability of the gain below the percent level. The resulting stability of the whole system is discussed, nicely demonstrating that silicon photo detectors are perfectly suited for the usage in Cherenkov telescopes, especially for long-term monitoring purpose.A. Biland, T. Bretz, J. Buß, V. Commichau, L. Djambazov, D. Dorner, S. Einecke, D. Eisenacher, J. Freiwald, O. Grimm, H. von Gunten, C. Haller, C. Hempfling, D. Hildebrand, G. Hughes, U. Horisberger, M.L. Knoetig, T. Krähenbühl, W. Lustermann, E. Lyard, K. Mannheim, K. Meier, S. Mueller, D. Neise, A.-K. Overkemping, A. Paravac, F. Pauss, W. Rhode, U. Röser, J.-P. Stucki, T. Steinbring, F. Temme, J. Thaele, P. Vogler, R. Walter and Q. Weitze
Observation of Pulsed γ-Rays Above 25 GeV from the Crab Pulsar with MAGIC
No full textOne fundamental question about pulsars concerns the mechanism of their pulsed electromagnetic emission. Measuring the high-end region of a pulsar's spectrum would shed light on this question. By developing a new electronic trigger, we lowered the threshold of the Major Atmospheric γ-ray Imaging Cherenkov (MAGIC) telescope to 25 giga-electron volts. In this configuration, we detected pulsed γ-rays from the Crab pulsar that were greater than 25 giga-electron volts, revealing a relatively high cutoff energy in the phase-averaged spectrum. This indicates that the emission occurs far out in the magnetosphere, hence excluding the polar-cap scenario as a possible explanation of our measurement. The high cutoff energy also challenges the slot-gap scenario
Die Ergänzungsbilanz | Grundlagen der Mitunternehmerbesteuerung und Folgerungen für die Fortentwicklung von Ergänzungsbilanzen
No full textDie Arbeit untersucht das richterrechtlich entwickelte Rechtsinstitut der Ergänzungsbilanz, das der Sicherstellung und Erfassung mitunternehmerbezogener Anschaffungskosten für eine Personengesellschaftsbeteiligung dient und die personenbezogene Zuordnung stiller Reserven dokumentiert. Besteht über die grundlegende Funktion von Ergänzungsbilanzen bei der Mitunternehmerbesteuerung noch Einigkeit, so sind viele dogmatische Grund- und Einzelfragen nach wie vor ungeklärt. Das gilt besonders für die Frage, nach welchen Modellen einmal gebildete Ergänzungsbilanzen eigentlich fortzuführen sind. Zur Lösung dieser Frage werden zunächst die Grundlagen der Mitunternehmerbesteuerung aufgearbeitet und neu vermessen und sodann das Rechtsinstitut der Ergänzungsbilanzen selbst eingängig untersucht. Auf dem Boden einer Würdigung verfassungsrechtlicher Grundprinzipien, einfach-gesetzlicher Vorgaben und von Theorien der Rechtsprechung entwickelt der Autor anschließend eigene Maßstäbe für die Fortentwicklung von Ergänzungsbilanzen.Die Arbeit wurde mit dem Promotionspreis 2023 der rechtswissenschaftlichen Fakultät der Universität zu Köln und dem Förderpreis der Esche Schümann Commichau Stiftung 2024 ausgezeichnet.»The German Concept of Inside and Outside Basis in Partnership Taxation. Fundamentals of Partnership Taxation and Conclusions for the Depreciation of Partnership Assets«: The thesis examines the German judicially developed concept of inside and outside basis in partnership taxation with special regard to the depreciation of assets. At the same time, the fundamentals of partnership taxation are reappraised and remeasured. The author develops his own standards for the depreciation of assets in partnership taxation based on an appraisal of basic principles of constitutional law, provisions of simple law, and theories of case law
The protein tyrosine kinases EpsB and PtkA differentially affect biofilm formation in <em>Bacillus subtilis</em>
No full textThe Gram-positive soil bacterium Bacillus subtilis is able to choose between motile and sessile lifestyles. The sessile way of life, also referred to as biofilm, depends on the formation of an extracellular polysaccharide matrix and some extracellular proteins. Moreover, a significant portion of cells in a biofilm forms spores. The first two genes of the 15-gene operon for extracellular polysaccharide synthesis, epsA and epsB, encode a putative transmembrane modulator protein and a putative protein tyrosine kinase, respectively, with similarity to the TkmA/PtkA modulator/kinase couple. Here we show that the putative kinase EpsB is required for the formation of structured biofilms. However, an epsB mutant is still able to form biofilms. As shown previously, a ptkA mutant is also partially defective in biofilm formation, but this defect is related to spore formation in the biofilm. The absence of both kinases resulted in a complete loss of biofilm formation. Thus, EpsB and PtkA fulfill complementary functions in biofilm formation. The activity of bacterial protein tyrosine kinases depends on their interaction with modulator proteins. Our results demonstrate the specific interaction between the putative kinase EpsB and its modulator protein EpsA and suggest that EpsB activity is stimulated by its modulator EpsA.</p
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