512 research outputs found
A Study of Thermally Radiant Williamson Nanofluid Over an Exponentially Elongating Sheet with Chemical Reaction Via Homotopy Analysis Method
No full textSynthesis of alkaline protease by catabolite repression-resistant Thermoactinomyces sp. E79 mutant
No full textThe production of alkaline protease from Thermoactinomyces sp. E79 was repressed by 0.2% (w/v) glucose in the medium. Catabolite repression-resistant mutant M1 was obtained by combined treatment of UV light and N-methyl-N'-nitro-N-nitrosoguanidine. The glucose uptake studies accomplished by [14C]glucose showed that the mutant has lost its ability for glucose uptake. The protease production by mutant M1 in the enzyme production medium was 62 U/mg, which was twice that of the wild-type strain.open
Statistical optimization of seed and induction conditions to enhance phytase production by recombinant Escherichia coli
No full textAn efficient fermentation process for the production of phytase was developed by statistical optimization from recombinant Escherichia coli BL21. The seed age and inoculum level highly influenced the expression of phytase gene by lactose induction. The optimum of 3.15-h seed culture age instead of an overnight culture and 4.8% (v/v) seed culture yielded high concentrations of cells at short intervals of time for efficient induction. Induction at a lactose concentration of 7.28 mM and at a cell optical density of 0.55 at 550 nm (equivalent to 0.23 g/l dry cell weight) was found to be most suitable in this system. The time of induction and lactose concentration significantly influenced phytase production. The optimized conditions were used to achieve 100% increase in phytase production (174 U/l · h) compared to production before optimizing the conditions.open
Sunitha Krishnan i jej walka z seksualnym niewolnictwem kobiet w Indiach
No full textThis article introduces the silhouette of Hindu activist Sunitha Krishan, has been strongly committed to the fight against sexual slavery in India for 20 years. It presents the circumstances of the fact that she started her business, initiatives which was undertaken by her and what was the results of her activities. In summary form the article presents stories that Krishnan has collected by seeing with victims – women and children. The author also essays the presentation of Hindu models of femininity and she demonstrates their influence on the current situation of women. This text is largery based on online sources, articles published on the information portals, interviews with Krishnan and record during the conference with her participation.Niniejszy artykuł przybliża sylwetkę hinduskiej aktywistki Sunithy Krishan, która od 20 lat jest silnie zaangażowana w walkę z seksualnym niewolnictwem w Indiach. Przedstawia okoliczności, w jakich doszło do rozpoczęcia jej działalności, inicjatywy przez nią podejmowane oraz rezultaty działań. W skrótowej formie prezentuje opowieści, które zebrała Krishnan spotykając się z pokrzywdzonymi kobietami i dziećmi. Autorka podejmuje również próbę przedstawienia hinduskich modeli kobiecości i wykazania jaki mają one wpływ na obecną sytuację kobiet. Tekst oparty jest w dużej mierze na źródłach internetowych, artykułach publikowanych na portalach informacyjnych, wywiadach przeprowadzonych z Krishnan oraz zapisie konferencji z jej udziałem.Udostępnienie publikacji Wydawnictwa Uniwersytetu Łódzkiego finansowane w ramach projektu „Doskonałość naukowa kluczem do doskonałości kształcenia”. Projekt realizowany jest ze środków Europejskiego Funduszu Społecznego w ramach Programu Operacyjnego Wiedza Edukacja Rozwój; nr umowy: POWER.03.05.00-00-Z092/17-00
Refolding and purification of Zymomonas mobilis levansucrase produced as inclusion bodies in fed-batch culture of recombinant Escherichia coli
No full textZymomonas mobilis levansucrase was overproduced by the fed-batch culture of recombinant Escherichia coli harboring a novel expression system that is constitutively expressed by the promoter from the Rahnella aquatilis levansucrase gene. Most of the levansucrase was produced as inclusion bodies in the bacterial cytoplasm, accounting for approximately 20% of the total cellular protein. Refolding after complete denaturation by high concentrations of urea or guanidine hydrochloride was not successful, resulting in large amounts of insoluble aggregates. During the development of the refolding method, it was found that direct solubilization of the inclusion bodies with Triton X-100 reactivated the enzyme, with a considerable refolding efficiency. About 65% of inclusion body levansucrase was refolded into active levansucrase in the renaturation buffer containing 4% (v/v) Triton X-100. The in vitro refolded enzyme was purified to 95% purity by single-step DEAE-Sepharose ion exchange chromatography. Triton X-100 was removed by this ion exchange chromatography.open
Platelet-rich fibrin: Evolution of a second-generation platelet concentrate
No full textPlatelet-rich plasma (PRP) is a platelet concentrate that has been used widely to accelerate soft-tissue and hard-tissue healing. The preparation of PRP has been described by several authors. Platelet-rich fibrin (PRF) was first described by Choukroun et al. in France. It has been referred to as a second-generation platelet concentrate, which has been shown to have several advantages over traditionally prepared PRP. Its chief advantages include ease of preparation and lack of biochemical handling of blood, which makes this preparation strictly autologous. This article describes the evolution of this novel platelet concentrate, referred to as PRF
Fault-tolerant pancyclicity of augmented cubes
No full textAs an enhancement on the hypercube Qn, the augmented cube AQ n, prosed by Choudum and Sunitha [S.A. Choudum, V. Sunitha, Augmented cubes, Networks 40 (2) (2002) 71–84], not only retains some favorable properties of Qn but also possesses some embedding properties that Qn does not. For example, AQ n is pancyclic, that is, AQ n contains cycles of arbitrary length for n � 2. This paper shows that AQ n remains pancyclic provided faulty vertices and/or edges do not exceed 2n − 3andn � 4
An analysis of concentration of sucrose, endogenous pH, and alteration in the plaque pH on consumption of commonly used liquid pediatric medicines
No full textBackground: Many parents are often unaware of the hidden, added sugars in many foods and drinks including pediatric liquid medicines; thus, hidden sugar in the form of pediatric medications has not been focused upon as cariogenic agents. Objective: (i) assess concentration of sucrose in six pediatric drugs, (ii) determine endogenous pH of these drugs, and (iii) estimate drop in the plaque pH in the oral cavity in first 30 minutes after consumption of the drugs. Materials and Methods: Ten adult volunteers with mean age of 22 years were double blinded for the study. Concentration of sucrose was assessed by volumetric method at Department of Chemical Branch of Engineering. Endogenous pH and drop in the plaque pH after consumption of the drugs were assessed using digital pH meter. Statistical analysis: SPSS software was used to assess the pH level at different time intervals and expressed as mean ± SD. Changes in pH were assessed by one-way ANOVA followed by Wilcoxons signed rank test. P-value was set at 0.05. Result: There were varying amounts of fermentable sucrose detected in the drugs; all the drugs were acidic. There is a significant drop of plaque pH after consumption of the drug. Conclusion: These sweeteners along with their low endogenic pH form a high cariogenic formulation. Thus, nonsucrose (noncariogenic) or sugar-free medications are needed to be prescribed along with proper oral hygiene care to the children under medication
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