3,684 research outputs found
The Ribosome Cooperates with the Assembly Chaperone pICln to Initiate Formation of snRNPs
No full textSummaryThe formation of macromolecular complexes within the crowded environment of cells often requires aid from assembly chaperones. PRMT5 and SMN complexes mediate this task for the assembly of the common core of pre-mRNA processing small nuclear ribonucleoprotein particles (snRNPs). Core formation is initiated by the PRMT5-complex subunit pICln, which pre-arranges the core proteins into spatial positions occupied in the assembled snRNP. The SMN complex then accepts these pICln-bound proteins and unites them with small nuclear RNA (snRNA). Here, we have analyzed how newly synthesized snRNP proteins are channeled into the assembly pathway to evade mis-assembly. We show that they initially remain bound to the ribosome near the polypeptide exit tunnel and dissociate upon association with pICln. Coincident with its release activity, pICln ensures the formation of cognate heterooligomers and their chaperoned guidance into the assembly pathway. Our study identifies the ribosomal quality control hub as a site where chaperone-mediated assembly of macromolecular complexes can be initiated
Assembly of RNPs: help needed
No full textThis extract was created in the absence of an abstract: In 1968 the Nomura lab in Madison made an astonishing observation: when ribosomal proteins were mixed under appropriate conditions with 16S rRNA, fully functional ribosomal 30S subunits were formed. Along with the subsequent total reconstitution of 50S subunits, it was established that even entire ribosomes could be assembled in vitro from their constituents. Neither energy in the form of nucleoside triphosphates, nor other “helping factors,” were required for this reaction. It was thus concluded that the structural information required for the formation of even complex macromolecules lies within its individual components itself and hence allows “self assembly.” This notion was soon supported by many other labs, which managed to assemble macromolecular RNPs in spontaneous reactions, including the signal recognition particle (SRP), spliceosomal subcomplexes (U snRNPs) and nucleolar RNPs (snoRNPs), to name just a few. These findings were in full accordance with the observations of the Anfinsen lab in the 1950s
Introduction
No full textSchedewie F, Grüner F, Utz R. Introduction. In: Fischer von Weikersthal F, Grüner F, Hohler S, Schedewie F, Utz R, eds. The Russian Revolution of 1905 in Transcultural Perspective. Identities, Peripheries, and the Flow of Ideas. Allan K. Wildman Group historical series. Vol 6. Bloomington: Slavica Publishers; 2013: 1-9
The Russian Revolution of 1905 in Transcultural Perspective. Identities, Peripheries, and the Flow of Ideas
No full textFischer von Weikersthal F, Grüner F, Hohler S, Schedewie F, Utz R, eds. The Russian Revolution of 1905 in Transcultural Perspective. Identities, Peripheries, and the Flow of Ideas. Allan K. Wildman Group historical series. Vol 6. Bloomington: Slavica Publishers; 2013
The Russian Revolution of 1905 at the Periphery: The Case of the Manchurian City of Harbin
No full textGrüner F. The Russian Revolution of 1905 at the Periphery: The Case of the Manchurian City of Harbin. In: Fischer von Weikersthal F, Grüner F, Hohler S, Schedewie F, Utz R, eds. The Russian Revolution of 1905 in Transcultural Perspective. Identities, Peripheries, and the Flow of Ideas. Allan K. Wildman Group historical series. Vol 6. Bloomington: Slavica Publishers; 2013: 175-196
Application of advanced notch stress approaches to assess fatigue strength of ship structural details: consolidation and dissemination of results
No full textThis report summarizes research activities carried out in compliance with the extension period of the research project proposed to and funded by the A. Von Humboldt Stiftung. Outline of the activities is reported in the work plan for the fifth period of exchange of the fellowship (extension) of the first author, at the beginning of the present report. Reference is made to previous reports describing activities of former periods.
Thereafter, activities are outlined as they were carried out during the stay in Hamburg of the first author.
Similarly to previous reports, this report is co-authored by Dipl.-Ing. Claas Fischer, actually Doktorand at TUHH and working on similar topics, as the reported research activities were carried out in strict cooperation
Application of advanced notch stress approaches to assess fatigue strength of ship structural details: benchmarks on complex geometries and laboratory tests
No full textThis report summarizes research activities carried out in compliance with Task #3, #4 and #5 of the research project proposed to and funded by the A. Von Humboldt Stiftung. Outline of the activities is reported in the work plan for the third period of exchange of the fellowship of the first author at the beginning of the present report, briefly summarizing the findings from the previous work and looking forward to future developments.
Thereafter, activities are outlined as they were carried out during the stay in Hamburg of the first author. Advices for future activities are also provided for both, numerical and experimental analyses. Updating of literature review about N-SIF based approaches is finally reported.
Similarly to a previous report, this report is co-authored by Dipl.-Ing. Claas Fischer, actually doktorand at TUHH and working on similar topics, as the reported research activities were carried out in strict cooperation
Application of advanced notch stress approaches to assess fatigue strength of ship structural details: analysis of tests and evaluation of approaches for complex geometries
No full textThis report summarizes research activities carried out in compliance with the extension period of the research project proposed to and funded by the A. Von Humboldt Stiftung. Outline of the activities is reported in the work plan for the fourth period of exchange of the fellowship (extension) of the first author, at the beginning of the present report. Reference is made to previous reports describing activities of former periods.
Thereafter, activities are outlined as they were carried out during the stay in Hamburg of the first author.
Similarly to previous reports, this report is co-authored by Dipl.-Ing. Claas Fischer, actually Doktorand at TUHH and working on similar topics, as the reported research activities were carried out in strict cooperation
Prayer Kaddish
No full textKadiš je kratka liturgijska molitva sa eshatološkim fokusom koja se više puta izgovara tokom verske službe (uglavnom na aramejskom) kojom se veliča i slavi Bog. Takođe je i molitva ožalošćenih tokom prve godine žalosti. Kadiš je za sve Jevreje, i pobožne i slobodumne, uzvišen i svet i možda najpopularnija molitva u celokupnoj jevrejskoj liturgiji. Dr Fischer smatra da su njen postanak, razvoj, sadržaj, značaj, jezik i odnos ka spasenju duše malo poznati i da samo moć navike ovoj molitvi daje prividnu snagu. Zbog toga u članku “Molitva Kadiš” želi da protumači sadržaj ove divne molitve i da širu javnost upozna sa njenom istorijom. Govoreći o nastanku ove molitve autor nas upoznaje sa verovatnim izvorima koji se odnose na citate iz Talmuda i Mišne kao i na jednu staru potresnu bajku iz vremena Rab Akibe. Kadiš je prvobitno napisan na hebrejskom jeziku pa se tako citira i pojavljuje u Talmudu. Kasnije je u Vavilonu hebrejski tekst preveden na aramejski ali su odlomci starog hebrejskog teksta očevidno zadržani u delu molitve, koji se i danas govori subotom i praznicima pri iznošenju Tore. Dr Fischer smatra da je neobično da se ova najpoznatija i najčešće izgovarana molitva slabo ili nikako može razumeti te stoga analizira svaki stih posebno dajući nam potrebna objašnjenja i tumačenja. Na kraju rada govori o promenama koje se su događale tokom vremena i poredi prvobitni i savremeni sadržaj Kadiša.Kaddish is a short liturgical prayer with an eschatological focus that is pronounced several times during a religious service (mostly in Aramaic) which praises and glorifies God. There is also the prayer of the grieving during the first year of mourning. For all Jews, both religious and free-spirited, Kaddish is sublime and holy, and perhaps the most popular prayer in the entire Jewish liturgy. Dr. Fischer believes that its origin, development, content, significance, language, and attitude towards the salvation of the soul are slightly known and that only the power of habit gives this prayer illusory strength. That is why in the article "Prayer Kaddish" he wants to interpret the content of this wonderful prayer and to acquaint the general public with its history. Speaking about the origin of this prayer, the author introduces us to probable sources that refer to quotations from the Talmud and the Mishnah, as well as to an old shocking fairy tale from the time of Rab Akiba. Kaddish was originally written in Hebrew, so it is quoted and appears in the Talmud. Later, in Babylon, the Hebrew text was translated into Aramaic, but excerpts from the old Hebrew text were apparently retained in the part of the prayer, which is still spoken today on Saturdays and holidays during the presentation of the Torah. Dr. Fischer finds it unusual that this most famous and most often pronounced prayer can be poorly or not at all understood and therefore analyzes each verse separately giving us the necessary explanations and interpretations. At the end of the paper, he talks about the changes that have taken place over time and compare the original and modern content of Kaddish.Dr Leopold Fischer (1886-1941) bio je rabin u Daruvaru a potom nadrabin u Vršcu. Uređivao je "Jevrejski almanah" godišnjak Saveza rabina Kraljevine SHS, koji je izlazio od 1925 do 1930. Bio je član Glavnoga odbora Saveza jevrejskih opština Jugoslavije i član Upravnoga odbora biblioteke i muzeja grada Vršca. Autor je knjiga i tekstova koji i danas predstavljaju važan izvor u talmudskim studijama. Bio je osoba izuzetnog obrazovanja, govorio je 9 jezika, imao dva doktorata, dopisivao se s brojnim poznatim i uglednim ličnostima (Šalom Alejhem, Šalom Aš, Rabindranat Tagore). Nakon sloma Kraljevine Jugoslavije uhvaćen je i sa ostalim beogradskim Jevrejima deportovan u logor Topovske Šupe u Beogradu, gdje je stradao.Dr. Leopold Fischer (1886-1941) was a rabbi in Daruvar and senior rabbi in Vršac. He was edited "Jewish Almanac" - the yearbook of the Association of Rabbis of the Kingdom of Serbs, Croats and Slovenes, which was published from 1925 to 1930. He was a member of the Main Board of the Federation of Jewish Communities of Yugoslavia and a member of the Board of the Library and Museum of Vršac. He is the author of books and texts that still represent an important source in Talmudic studies. He was a person of exceptional education, he spoke nine languages, he had two doctorates, and he corresponded with many famous and respectable people (Shalom Aleichem, Shalom Ash, Rabindranath Tagore). After the collapse of the Kingdom of Yugoslavia, he was captured and deported with other Belgrade Jews to the Topovske Šupe camp in Belgrade, where he died
Molecular cloning and targeted deletion of PEP2 which encodes a novel aspartic proteinase from Aspergillus fumigatus
No full textAn aspartic proteinase PEP2 [EC 3.4.23.25] was purified from a cell wall fraction of Aspergillus fumigatus. The enzyme, which showed a broad range of activity from pH 2.0 to 7.0 and migrated as a single band of 39 kDa in SDS-PAGE, was not detected in the culture supernatant. A specific gene probe was designed on the basis of the N-terminal sequence of the native protein, and the PEP2 genomic and cDNA were isolated from corresponding libraries. The deduced amino acid sequence of PEP2 consists of 398 amino acids. A signal sequence of 18 amino acids and a proregion of another 52 amino acids were identified. The mature protein consists of 328 amino acids which include the two DTG-motifs of the active site common to almost all pepsin-like enzymes; PEP2 showed a 64% identity with the vacuolar proteinase A (PrA), of Saccharomyces cerevisiae, and an 88% identity with PEPE, an aspartic proteinase of Aspergillus niger. Recombinant PEP2 was overexpressed in Pichia pastoris and the active enzyme was secreted into the culture supernatant. Targeted deletion of PEP2 did not affect vegetative growth or cell and colony morphology. Identification of proteinases, such as PEP2, which are apparently associated with the Aspergillus cell wall raises new interest in these molecules with respect to their possible function in the pathogenesis of invasive aspergillosis
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