1,720,960 research outputs found
Purifikasi dan Karakterisasi α-amilase Termostabil dari Bacillus stearothermophilus TII-12
Full text link<p>Purification and Characterization of Thermostable<br />α-amylase from Bacillus stearothermophilus TII-12. Puji<br />Lestari, Nur Richana, Abdul A. Darwis, Khaswar Syamsu,<br />and Untung Murdiyatmo. Thermostable α-amylase is a<br />potential enzyme employed in the starch processing and<br />widely used in food industries, but this enzyme is still<br />imported. The local enzyme production would be more<br />economist and useful for its broad applications. Here we<br />report α-amylase from indigenous bacteria TII-12 which was<br />purified and characterized, as well as analyzed its hydrolysis<br />product on cassava starch. The enzyme of Bacillus<br />stearothermophilus TII-12 partially purified by ultrafiltration,<br />acetone precipitation and gel filtration (Sephadex G-100)<br />showed the reduced total activity, total protein and yield, but<br />increased the specific activity. The enzyme had a Km of 1,06<br />mg/ml and Vmax of 1,21 mol/min, with optimal activity at pH 7<br />and 90oC. An apparent molecular mass was of 192.932,8<br />Dalton, as estimated by Native-Polyacrylamide Agarose Gel<br />electrophoresis. Its activity was inhibited by the divalent<br />cation chelator such as EDTA and CuSO4 but activated by<br />calcium ion. Hydrolysis products of this enzyme on cassava<br />starch were glucose, dextrin, maltose and oligosaccharides.<br />After 24 hours of hydrolysis, the concentration of glucose<br />and maltose reached 51.970 and 10.090 ppm, respectively.<br />The thermostable α-amylase of TII-12 is an endo-α-amylase<br />and prospective to be applied on starch liquefaction with<br />high temperature process.</p></jats:p
Produksi, isolasi dan karakterisasi enzim dekstranase dari Arthrobacter sp. B7
No full textDextranase enzyme has been purified and characterized from Arthrobacter sp. B7. This enzyme was purified from the culture
supernatant of Arthrobacter sp. B7 by procedure of native PAGE. The molecular size of the enzyme was estimated 72,5 kDa by SDSPAGE.
The N-terminal amino acid sequence of this enzyme determined using Edman degradation techniques were APVTADVGNLHT.
SDS-PAGE and native-PAGE analysis revealed that the enzyme molecule consisted of one sub-unit
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
- …
