11,772 research outputs found
Service-oriented models for audiovisual content storage
No full textWhat are the important topics to understand if involved with storage services to hold digital audiovisual content? This report takes a look at how content is created and moves into and out of storage; the storage service value networks and architectures found now and expected in the future; what sort of data transfer is expected to and from an audiovisual archive; what transfer protocols to use; and a summary of security and interface issues
The VvBAP1 gene is identified as a potential inhibitor of cell death in grape berries
No full textCell death (CD) in Vitis vinifera L grape berries, exemplified in Shiraz, occurs late in ripening influencing yield, berry and wine quality. Here we isolated and functionally characterised a BON1-associated gene, VvBAP1 from Shiraz berries, encoding a small protein with a C2 domain. VvBAP1 transcript increased during fruit development from veraison to harvest, and was significantly inhibited by drought stress 92 days after flowering when CD normally begins. This was correlated with high CD in Shiraz berries. The agrobacterium-mediated transient expression of VvBAP1 in tobacco leaves led to a decrease in electrolyte leakage and downregulated a marker gene (Hsr203J) for cell death. Expressing VvBAP1 in yeast (Saccharomyces cerevisiae) also alleviated cell death induced by hydrogen peroxide (H₂O₂). Overexpression of VvBAP1 in Arabidopsis increased resistance to H₂O₂ and reduced CD due to higher expression of genes involved in anti-oxidative responses. Arabidopsis overexpressing VvBAP1 displayed higher tolerance to drought accompanied by upregulation of antioxidant-related gene expression. VvBAP1 complemented an Arabidopsis bap1 knockout by abolishing its CD phenotypes. These results indicate that VvBAP1 may play a role in alleviating CD in grape berries and its downregulation under drought stress may be responsible for the generally observed increase in CD within the berry.Shifeng Cao, Zeyu Xiao, Vladimir Jiranek and Stephen D. Tyerma
Barley phosphate transporter 1;6 shows broad inorganic anion transport activity when expressed in Xenopus laevis oocytes
No full textXVI IPNC 2009, Sacramento, CaliforniaPreuss, Christian P., Huang, Chun Y., Gilliham, Matthew and Tyerman, Stephen D.http://escholarship.org/uc/ipnc_xv
Cytosolic GABA inhibits anion transport by wheat ALMT1
No full textFirst published: 07 October 2019.
Corrected by: Corrigendum: Cytosolic GABA inhibits transport by wheat ALMT1 (vol 225, pg 671, 2020), in Early view - https://doi.org/10.1111/nph.18214. Since its publication, the authors of Long et al. (2020) have brought to our attention an error in their article. In the Acknowledgements section, the support for the work from the Australian Research Council Centre of Excellence funding to Matthew Gilliham and Stephen Tyerman was incorrectly listed under the number ‘CE14010008’. The correct funding number is ‘CE140100008’, and the corrected Acknowledgements section is shown below. We apologize to our readers for this mistake.Anion transport by Aluminium-activated malate transporter (ALMT) proteins is negatively regulated by gamma-aminobutyric acid (GABA), which increases in concentration during stress. Here, the interaction between GABA and wheat (Triticum aestivum, Ta) TaALMT1 heterologously-expressed in Xenopus laevis oocytes was investigated. GABA inhibited anion transport by TaALMT1 in membrane patches from the cytosolic, not extracellular membrane face, via a reduction in open probability (NPopen ), not an inhibition of channel current magnitude. TaALMT1 currents in patches frequently exhibited rundown with complete removal of cytosolic factors, but were partially sustained by protein kinase C dependent phosphorylation. When applied to whole oocytes a GABA-analogue-BODIPY conjugate inhibited TaALMT1 anion currents from the cytoplasmic face only, whereas free GABA inhibited from both the inside and outside consistent with GABA traversing the TaALMT1 pore then acting from the inside. We propose GABA does not competitively inhibit ALMT conductance through the same pore but rather leads to an allosteric effect, reducing anion channel opening frequency. Across plants GABA is a conserved regulator of anion transport via ALMTs - a family with numerous physiological roles beyond Al3+ tolerance. Our data suggests that a GABA-ALMT interaction from the cytosolic face has the potential to form part of a novel plant signalling pathway.Yu Long, Stephen D. Tyerman, Matthew Gilliha
Molybdate transport through the plant sulfate transporter SHST1
No full textCopyright © 2008 Federation of European Biochemical Societies. Published by Elsevier BV. All rights reserved.Molybdenum is an essential micronutrient required by plants. The mechanism of molybdenum uptake in plants is poorly understood, however, evidence has suggested that sulfate transporters may be involved. The sulfate transporter from Stylosanthes hamata, SHST1, restored growth of the sulfate transport yeast mutant, YSD1, on media containing low amounts of molybdate. Kinetic analysis using 99MoO4(2-) demonstrated that SHST1 enhanced the uptake of molybdate into yeast cells at nM concentrations. Uptake was not inhibited by sulfate, but sulfate transport via SHST1 was reduced with molybdate. These results are the first measurement of molybdate transport by a characterised plant sulfate transport protein.Kate L. Fitzpatrick, Stephen D. Tyerman, Brent N. Kaiserhttp://www.sciencedirect.com/science/journal/0014579
AC-6-U.S. Naval Planes Flying in Formation, Langley Field, VA/Thank-You Card from Stephen Tury to the Hungarian Defense Council.
No full textThis postcard, which depicts U.S. Naval planes flying in formation, was sent to the Hungarian Defense Council by Private Stephen Tury. The Council was organized in New Brunswick by leaders of local Hungarian churches and societies. During the Second World War it sent supplies, such as the carton of cigarettes Tury is thanking it for, to members of the military of Hungarian descent from the New Brunswick area
Author Stephen Flynn Discusses Resiliency
No full textCenter for Homeland Defense and Security, PRESS RELEASESOn September 25, Author Stephen E. Flynn stopped by the Center’s National Capital Region campus to speak with CHDS Master’s degree students about his latest book, answer questions and discuss..
Letter from Carl Hayden to Stephen Mather, National Park Service
No full textLetter from Carl Hayden to Stephen Mather regarding the sale of Bass properties
Effect of different host plants on the growth of the root hemiparasite Santalum acuminatum (quandong)
No full text© CSIRO 2002In recent years, the root hemiparasite Santalum acuminatum (quandong) has become an increasingly important commercial crop within the indigenous foods market. Relatively little is known of the significance of the host species on quandong growth and development. This paper presents data from a glasshouse pot experiment showing the effect of 4 different host species on the growth of quandong. Quandong growth, as measured by height and dry-mass accumulation, was significantly improved when grown in the presence of a host plant compared with being grown alone. Quandong grown with a host achieved an average height of 45 cm, while those grown alone grew up to only 37 cm. The host species also had an impact on the growth of quandong. Quandong had no detrimental effect on the growth of its host plant. Quandong grown with Myoporum parvifolium and Atriplex nummularia accumulated a significantly greater amount of dry biomass than quandong grown alone.Beth R. Loveys, Stephen D. Tyerman and Brian R. Lovey
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