10 research outputs found
Microbial Culture Collections: The Essential Resources for Life
No full textMicrobial culture collections are crucial resource centres providing microbial materials. They act as repositories for microbial strains as part of patent deposits, confidential services to store key organisms for research, industry and society and sources of microorganisms cited in scientific papers that can be used in the confirmation of results and for further studies. Microbial culture collections are considered as libraries, but instead of books they hold microorganisms. The first culture collection was established by Prof. Frantisek Kral in 1890 at the German University of Prague. After this collection, many culture collections established. Now there are 568 culture collections over the world. These collections categorize as national collections which provide extensive services or small collections which supply strains or related services by special agreement. Microorganisms provide many solutions to world problems in public health, food, environment and poverty and they are extremely important for various ecosystems
Molecularly Imprinted Biomimetic Surface Plasmon Resonance Sensor For Hormone Detection
No full textIn this study, 17 beta-estradiol (E2) was performed by surface plasmon resonance (SPR) based on molecularly imprinted technique. In the first, SPR sensor was prepared by the formation of E2 imprinted poly(2-hydroxyetyl methacrylate) / (N-methacryloyl-(L)- leucine methyl esters) (PHEMALM) on the modified gold surface of SPR chip. Also, non-imprinted SPR chip was prepared by the same method at the E2 imprinted PHEMALM (E2-MIPHEMALM-SPR) chip without E2. E2-MIPHEMALM-SPR and non-imprinted (NIPHEMALM-SPR) chips were characterized with Fourier transform infrared spectrophotometry (FTIR), ellipsometry, atomic force microscopy (AFM) and contact angle measurements. After that, SPR chips were completed to SPR system to investigate kinetic properties for E2. The sensing ability of E2-MIPHEMALM-SPR chip was investigated with 20-10000 ng/mL concentrations of 17 beta-estradiol solutions. The E2-MIPHEMALM-SPR chip was showed more selectivity for 17 beta-estradiol than NIPHEMALM-SPR chip. To show the selectivity of E2-MIPHEMALM-SPR chip competitive adsorption of 17 beta-estradiol, cholesterol and stigmasterol were investigated. E2-MIPHEMALM-SPR chip was investigated ten times with same concentrations of 17 beta-estradiol solution to show reuse of the chip. The results showed that the E2-MIPHEMALM-SPR chip has high selectivity for 17 beta-estradiol.WoSScopu
The fabrication of nanosensor-based surface plasmon resonance for IgG detection
No full textPoly(2-hydroxyethyl methacrylate)/3-(2-imidazoline-1-yl) propyl(triethoxysilane) (PHEMA/IMEO) nanoparticles were attached on surface plasmon resonance (SPR) sensor for the real-time detection of human immunoglobulin G (IgG) in human serum. The PHEMA/IMEO nanoparticles-attached SPR sensor was characterized by Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), and contact angle measurements. IgG detection studies were performed using aqueous IgG solutions at different concentrations. In order to show the selectivity and specificity of the SPR sensor, competitive kinetic analyses were performed using IgG, albumin, hemoglobin in singular and competitive manner. Finally, IgG detection in human serum was carried out
In vitro Biological Activities of Different Extracts from Alcea dissecta
No full textObjective: Alcea genus belongs to Malvaceae family and this genus is represented by 85 taxa in the world and 21 taxa in the Flora of Turkey. The flowers of Alcea genus contain plenty of mucilage and are used for the treatment of respiratory diseases such as asthma and cough. Alcea dissecta is known as 'Govik, Hiro, Hero' in Turkey, and the flowers and leaves of this species have been used in the treatment of asthma, injury, colds, and gastrointestinal diseases in Turkey. To the best of our knowledge, there is no report on the effect of extraction methods on the biological activity of this plant. In addition, although this species is being used as a medical plant, there is no study of the antioxidant, antiurease, esterase, and anticholinesterase activity of the plant. Therefore, the aim of this study was to evaluate in vitro antioxidant, anti-urease, esterase, anticholinesterase activities of Alcea dissecta using a variety of extracts. Methods: The antioxidant activities of different extracts were examined by DPPH, ABTS, FRAP, and CUPRAC methods. The total phenolic compounds contained in the extracts were determined using the Folin-Ciocalteu reagent (FCR) method. Anti-urease and anticholinesterase activities of different extracts were evaluated by indophenol and Ellman methods respectively. In addition, esterase activities of plant extracts were determined. Results: In the present study, ethanol:water (1:1, v/v) and chloroform extracts obtained maceration method showed stronger DPPH and ABTS radical scavenging activity than other extracts. The chloroform extract obtained Soxhlet method was found to have higher FRAP and CUPRAC values than other extracts. It was also found that the ethanol extract obtained maceration method showed the most potent anti-urease and anticholinesterase activity. According to the results, the strongest inhibitory effect on both hCA I and II isoenzymes was shown by the petroleum ether extract obtained Soxhlet method. Conclusion: As a result, it was determined that different plant extracts have antioxidant, anti-urease, esterase, anticholinesterase activities. In addition, the data obtained from this study will shed light on future research on the biological activities of this species
Composite cryogels for lysozyme purification
No full textBeads-embedded novel composite cryogel was synthesized to purify lysozyme (Lyz) from chicken egg white. The poly(hydroxyethyl methacrylate-N-methacryloyl-L-phenylalanine) (PHEMAPA) beads of smaller than 5 mu m size were synthesized by suspension polymerization and then embedded into a poly(hydroxyethyl methacrylate) (PHEMA)-based cryogel column. The PHEMAPA bead-embedded cryogel (BEC) column was characterized by swelling tests, scanning electron microscopy (SEM), surface area measurements by the Brunauer-Emmett-Teller (BET) method, elemental analysis, and flow dynamics. The specific surface area of the PHEMAPA BEC was found as 41.2 m(2)/g using BET measurements. Lyz-binding experiments were performed using aqueous solutions in different conditions such as initial Lyz concentration, pH, flow rate, temperature, and NaCl concentration of an aqueous medium. The PHEMAPA BEC column could be used after 10 adsorption-desorption studies without any significant loss in adsorption capacity of Lyz. The PHEMAPA BEC column was used to purify Lyz from chicken egg white, and gel electrophoresis was used to estimate the purity of Lyz. The chromatographic application of the PHEMAPA BEC column was also performed using fast protein liquid chromatography. (C) 2014 International Union of Biochemistry and Molecular Biology, Inc
Dimensional Accuracy of Porous Structures Manufactured Using Air Controller
No full text© 2023, The Author(s), under exclusive license to Springer Nature Switzerland AG.Today, the additive manufacturing (AM) method is used in aerospace, defense, and biomedical fields due to its advantages. However, it is important to be able to control the production environment in these production methods. This study investigates the effects of the air control unit (ACU) in production with the fused deposition modeling (FDM) method on dimensional accuracy. Gyroid and body-centered cubic (BCC) porous structures were produced in an AC and non-AC environment. After production, the dimensional accuracy and surface quality of the porous structure structures were investigated. Significant surface defects were observed in the porous structure structures produced in a non-AC environment. Better quality surfaces were obtained in the productions carried out by providing AC. In addition, in the results obtained, it was observed that there was an average of 2% deviation in terms of dimensions in the productions carried out using the ACU and an average of 2.5% deviation in the productions without ACU
Nationwide prospective audit for the evaluation of appendicitis risk prediction models in adults: Right iliac fossa treatment (RIFT) - Turkey
No full textBackground: Appendicitis is the most prevalent surgical emergency. The negative appendicectomy rate and diagnostic uncertainty are important concerns. This study aimed to assess the effectiveness of current appendicitis risk prediction models in patients with acute right iliac fossa pain. Methods: A nationwide prospective observational study was conducted, including all consecutive adult patients who presented with right iliac fossa pain. Diagnostic, clinical and negative appendicectomy rate data were recorded. The Alvarado score, Appendicitis Inflammatory Response (AIR), Raja Isteri Pengiran Anak Saleha Appendicitis (RIPASA) and Adult Appendicitis Score systems were calculated with collected data to classify patients into risk categories. Diagnostic value and categorization performance were evaluated, with use of risk category-based metrics including 'true positive rate' (percentage of appendicitis patients in the highest risk category), 'failure rate' (percentage of patients with appendicitis in the lowest risk category) and 'categorization resolution' (true positive rate/failure rate). Results: A total of 3358 patients from 84 centres were included. Female patients were less likely to undergo surgery than men (71.5% versus 82.5% respectively; relative risk 0.866, 95% c.i. 0.834 to 0.901, P 0.001); with a three-fold higher negative appendicectomy rate (11.3% versus 4.1% respectively; relative risk 2.744, 95% c.i. 2.047 to 3.677, P 0.001). Ultrasonography was utilized in 56.8% and computed tomography in 75.2% of all patients. The Adult Appendicitis Score had the best diagnostic performance for the whole population; however, only RIPASA was significant in men. All scoring systems were successful in females patients, but Adult Appendicitis Score had the highest area under the receiver operating characteristic curve value. The RIPASA and the Adult Appendicitis Score had the best categorization resolution values, complemented by their exceedingly low failure rates in both male and female patients. Alvarado and AIR had extremely high failure rates in men. Conclusion: The negative appendicectomy rate was low overall, but women had an almost three-fold higher negative appendicectomy rate despite lower likelihood to undergo surgery. The overuse of imaging tests, best exemplified by the 75.2% frequency of patients undergoing computed tomography, may lead to increased costs. Risk-scoring systems such as RIPASA and Adult Appendicitis Score appear to be superior to Alvarado and AIR. © 2024 The Author(s). Published by Oxford University Press on behalf of BJS Foundation Ltd.Tibbi Akademi
