196,163 research outputs found
Phenotypic characterization of the <i>tupA</i> mutants.
No full text<p>(a) Ten thousand spores of UV mutant RD15.8#36, the parental strain RD15.8, the complemented mutant RD15.8#36/pAn14g00140, the full deletion mutant Δ<i>tupA</i> and wild-type N402, were spotted and growth was monitored on MM-glucose-nitrate plates or MM/glucose-acetamide plates acetamide (first vertical column or MM-glucose plates (if not stated differently) at the indicated temperature for 3 days. (b) DIC- and fluorescent pictures of parental strain (RD15.8), the <i>tupA</i> mutant, and the <i>tupA</i> deletion strain in the RD15.8 background after growth for 20 hours at 30°C in MM with casamino acids.</p
Musyawarah Lingkungan Hidup sebagai Strategi Penyelesaian Kerusakan Lingkungan di Desa Tupa
No full textTujuan penulisan ini adalah mensosialisasikan secara tertulis tentang Musyawarah Lingkungan Hidup sebagai salah satu cara penyelesaian kerusakan lingkungan di Desa Tupa yang dilakukan oleh Tim Pengabdian Fakultas Hukum Universitas Negeri Gorontalo. Musyawarah Lingkungan Hidup merupakan strategi yang diatur dalam draf Peraturan Desa Tupa tentang Pelestarian Lingkungan Hidup sebagai perwujudan dari hasil yang ingin dicapai dalam pengabdian, yaitu terbentuknya dokumen kebijakan pemerintah desa tentang bagaimana penyelesaian kerusakan lingkungan dan kesadaran hukum masyarakat Desa Tupa Kecamatan Bulango Utara. Metode yang digunakan dalam mencapai tujuan tersebut adalah (1) Pendampingan Rancang Bangun Strategi Penyelesaian Kerusakan Lingkungan Desa Tupa; (2) Penyuluhan, Sosialisasi dan Diseminasi Percepatan Strategi Penyelesaian Kerusakan Lingkungan dan Kesadaran Hukum Masyarakat Desa Tupa; (3) Launching Pembentukan Kelompok Gerakan Masyarakat Sadar Hukum Desa Tupa. Dalam pelaksanaan Program Pengabdian dapat ditarik kesimpulan bahwa meskipun telah terdapat beberapa peraturan baik di tingkat pusat maupun daerah terkait pengelolaan lingkungan hidup dan pengelolaan hutan, namun peraturan tersebut belum dapat mengatasi kerusakan lingkungan di Desa Tupa. Selan itu, peran serta semua pihak terkait, khususnya masyarakat merupakan poin utama dalam penyelesaian kerusakan lingkungan. Terakhir, pembentukan Musyawarah Lingkungan Hidup sebagai wadah masyarakat Desa Tupa untuk melestarikan lingkungan hidup merupakan salah satu strategi yang dapat digunakan menyelesaikan permasalahan kerusakan lingkungan di Desa Tupa. The purpose of this writing is to socialize in writing about the Environmental Consultation as a way to resolve environmental damage in Tupa Village, which was carried out by the Community Service Team of the Faculty of Law, State University of Gorontalo. The Environmental Deliberation is a strategy regulated in the draft Tupa Village Regulation on Environmental Preservation as a manifestation of the results to be achieved in community service, namely the formation of a village government policy document on how to resolve environmental damage and raise the legal awareness of the people of Tupa Village, North Bulango District. The methods used in achieving these objectives are: (1) assistance in the design of environmental damage settlement strategies for Tupa Village; (2) Counseling, socialization, and dissemination of the acceleration of environmental damage settlement strategies and legal awareness of the Tupa Village community; (3) Launching of the Formation of the Tupa Village Law Aware Community Movement. In the implementation of the Community Service Program, it can be concluded that although there have been several regulations both at the central and regional levels related to environmental management and forest management, these regulations have not been able to overcome environmental damage in Tupa Village. In addition, the participation of all related parties, especially the community, is the main point in solving environmental damage. Finally, the formation of an environmental conference as a forum for the Tupa Village community to preserve the environment is one of the strategies that can be used to solve the problem of environmental damage in Tupa Village
Overview of mutations in <i>A niger tupA</i> mutants.
No full text<p>Overview of mutations in <i>A niger tupA</i> mutants.</p
Developmental genes up-regulated in the <i>tupA</i> mutant.
No full text<p>Developmental genes up-regulated in the <i>tupA</i> mutant.</p
The Transcriptional Repressor TupA in Aspergillus niger Is Involved in Controlling Gene Expression Related to Cell Wall Biosynthesis, Development, and Nitrogen Source Availability.
No full textThe Tup1-Cyc8 (Ssn6) complex is a well characterized and conserved general transcriptional repressor complex in eukaryotic cells. Here, we report the identification of the Tup1 (TupA) homolog in the filamentous fungus Aspergillus niger in a genetic screen for mutants with a constitutive expression of the agsA gene. The agsA gene encodes a putative alpha-glucan synthase, which is induced in response to cell wall stress in A. niger. Apart from the constitutive expression of agsA, the selected mutant was also found to produce an unknown pigment at high temperatures. Complementation analysis with a genomic library showed that the tupA gene could complement the phenotypes of the mutant. Screening of a collection of 240 mutants with constitutive expression of agsA identified sixteen additional pigment-secreting mutants, which were all mutated in the tupA gene. The phenotypes of the tupA mutants were very similar to the phenotypes of a tupA deletion strain. Further analysis of the tupA-17 mutant and the ΔtupA mutant revealed that TupA is also required for normal growth and morphogenesis. The production of the pigment at 37°C is nitrogen source-dependent and repressed by ammonium. Genome-wide expression analysis of the tupA mutant during exponential growth revealed derepression of a large group of diverse genes, including genes related to development and cell wall biosynthesis, and also protease-encoding genes that are normally repressed by ammonium. Comparison of the transcriptome of up-regulated genes in the tupA mutant showed limited overlap with the transcriptome of caspofungin-induced cell wall stress-related genes, suggesting that TupA is not a general suppressor of cell wall stress-induced genes. We propose that TupA is an important repressor of genes related to development and nitrogen metabolism
Cell wall biosynthetic genes down-regulated in the <i>tupA</i> mutant.
No full text<p>Cell wall biosynthetic genes down-regulated in the <i>tupA</i> mutant.</p
Cell wall biosynthetic genes up-regulated in the <i>tupA</i> mutant.
No full text<p>Cell wall biosynthetic genes up-regulated in the <i>tupA</i> mutant.</p
GO terms (Biological Process) enriched among down-regulated genes in the <i>tupA</i> strain.
No full text<p>GO terms (Biological Process) enriched among down-regulated genes in the <i>tupA</i> strain.</p
Predicted GPI-anchored cell wall protein-encoding genes down-regulated in the <i>tupA</i> mutant.
No full text<p>Predicted GPI-anchored cell wall protein-encoding genes down-regulated in the <i>tupA</i> mutant.</p
Predicted (GPI-anchored) cell wall protein encoding genes up-regulated in the <i>tupA</i> mutant.
No full text<p>Predicted (GPI-anchored) cell wall protein encoding genes up-regulated in the <i>tupA</i> mutant.</p
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