213 research outputs found

    The Production of Functional Sex-Reversed Male Rainbow Trout (Oncorhynchus mykiss)

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    Arslan, Tulin/0000-0001-9661-045XWOS: 000302198700010In this study, appropriate hormonal sex reversal procedure for the production of functional sex-reversed male rainbow trout (Oncorhynchus mykiss) brooders with intact sperm ducts was investigated. For this purpose, 4 groups of experiment were conducted. In oral administration group, free swimming larvae starting from the first feeding were fed 1.0-3.0 mg 17 alpha-methyltestosterone kg diets for 600 degree-days. In the remaining experimental groups, 0.5-1.0 mg l(-1) 17 alpha-methyltestosterone, 11 beta-hidroksiandrostenedione or 17 alpha-nnethyldihydrotestosterone were administered throughout the sac fry stage (370-560 degree-days post fertilization) as short (2 h) single, 48 h apart 2-3 or 1 week apart double baths. Results of the study demonstrated that appropriate androgen procedure for the production of functionally masculinized rainbow trout females should be covering the 470-800 degree-days post fertilization, but it could be periodic baths or periodic baths and oral administration of low concentrations, at low concentrations (<= 1 mg kg(-1), feed) oral administration of 17 alpha-methyltestosterone could yield high ratios of functional sex reversed males

    Effects of vitamin E on growth and reproductive performance of pindani (Pseudotropheus socolofi Johnson, 1974)

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    Arslan, Tulin/0000-0001-9661-045XWOS: 000471749800009With the multibillion dollar worth global market, ornamental fish trade provides important business opportunities to aquaculturist. Pindani (Pseudotropheus socolofi) is one of the most popular freshwater fish species that is cultured for this market. However, we know little about its' nutrition and its' culture suffers from low egg and fry production numbers. Among many different nutritional components, essential vitamins, such as vitamin E, could have important impacts on reproductive performance of fishes. In this study, the effects of different dietary vitamin E levels on growth and reproductive performance of pindani were evaluated through two consecutive experiments, encompassing both juvenile and adult stages. Three different levels of alpha-tocopherol were incorporated into a commercially formulated diet at the rate of 50, 100 and 150 mg/kg. Control diet contained 121.3 mg alpha-tocopherol/kg. Elevating dietary a-tocopherol level above 121.3 mg/kg did not improve growth or feed utilization values of juvenile or adult pindani. However, reproductive performance of pindani was significantly (P 100 mg/kg provided no further improvement, but significantly reduced the relative fecundity of females. These results of the study were discussed in relation to dietary PUFA level, degree of parental care and alpha-tocopherol usage and regeneration balance. At the end, we concluded that a dietary alpha-tocopherol supplementation level of 219.3 mg/kg in a diet with 9% total lipid and 22.5-24.3% PUFA content could substantially improve the breeding performance of pindani and production efficiency of their culture.Mugla Sitki Kocman University, Mugla, TurkeyMugla Sitki Kocman University [BAP 2013/135]This study was funded by Mugla Sitki Kocman University, Mugla, Turkey (Contract # BAP 2013/135). We are also grateful to Dr. Fatih Basaran of ArtAkua Inc., Izmir, Turkey for providing all the feeds used in this study

    Crystal Structures of Cobalamin-Independent Methionine Synthase (MetE) from Streptococcus mutans: A Dynamic Zinc-Inversion Model

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    Cobalamin-independent methionine synthase (MetE) catalyzes the direct transfer of a methyl group from methyltetrahydrofolate to L-homocysteine to form methionine. Previous studies have shown that the MetE active site coordinates a zinc atom, which is thought to act as a Lewis acid and plays a role in the activation of thiol. Extended X-ray absorption fine structure studies and mutagenesis experiments identified the zinc-binding site in MetE from Escherichia coli. Further structural investigations of MetE from Thermotoga maritima lead to the proposition of two models: &quot;induced fit&quot; and &quot;dynamic equilibrium&quot;, to account for the catalytic mechanisms of MetE. Here, we present crystal structures of oxidized and zinc-replete MetE from Streptococcus mutans at the physiological pH. The structures reveal that zinc is mobile in the active center and has the possibility to invert even in the absence of homocysteine. These structures provide evidence for the dynamic equilibrium model. (C) 2011 Elsevier Ltd. All rightshttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000295496500012&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Biochemistry &amp; Molecular BiologySCI(E)PubMed7ARTICLE4688-69741

    Prospects for Single- and Di-Higgs Measurements at the HL-LHC with ATLAS

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    The large dataset of about 3 ab1^{-1} that will be collected at the High Luminosity LHC (HL-LHC) will be used to measure Higgs boson processes in detail. Studies based on current analyses have been carried out to understand the expected precision and limitations of these measurements. The large dataset will also allow for better sensitivity to di-Higgs processes and the Higgs boson self coupling. This proceeding presents the prospects for Higgs and di-Higgs results with the ATLAS detector at the HL-LHC

    Prospects for Single- and Di-Higgs Measurements at the HL-LHC with ATLAS

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    The large dataset of about 3 ab^{-1} that will be collected at the High Luminosity LHC (HL-LHC) will be used to measure Higgs boson processes in detail. Studies based on current analyses have been carried out to understand the expected precision and limitations of these measurements. The large dataset will also allow for better sensitivity to di-Higgs processes and the Higgs boson self coupling. This talk will present the prospects for Higgs and di-Higgs results with the ATLAS detector at the HL-LHC

    An IBC and certificate based hybrid approach to WIMAX security

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    WiMAX is a promising technology that provides high data throughput with low delays for various user types and modes of operation. These advantages make WiMAX applicable both for infrastructure purposes and end-client usage. Since WiMAX is presented as a network framework and a last-mile technology, it is believed to be capable of handling a wide range of usage scenarios. For example, while the end users have an opportunity to use WiMAX as the primary connection medium for acquiring services such as on-demand video streaming, VoIP connections and mobile bank transactions, the service providers may use it for data relaying purposes among access points. To meet the technical requirements of these various scenarios, majority of the WiMAX research has been conducted on physical and MAC layers; however little has been invested in a comprehensive and efficient security solution, which has resulted in a wide range of security weaknesses and reactive solutions. Many security problems remain to be addressed in different modes and for different user types even in the final security standard of WiMAX, PKMv2. In this thesis, we present a hybrid security solution combining Identity-Based Cryptography (IBC) and certificate based approaches to overcome the existing security problems of WiMAX without degrading service quality. IBC has potential benefits that can provide enhancements to the overall security and efficiency of the security standard. One such enhancement is combining user identity with the public key and therefore eliminating the public key distribution load from the network. However, IBC has a few caveats, such as the necessity of a secure medium to distribute private keys. To compensate for these disadvantages, in this study, IBC is combined with certificate-based security. As a result, the benefits of IBC are maintained while the disadvantages are eliminated. Using the hybrid approach, this study also aims to clarify the key revocation procedures and key lifetimes of WiMAX. To achieve this goal, key renewal intervals are examined and corresponding lifetimes are assigned to the credentials missing in both PKMv2 and PKMv1. Additionally, the key distribution procedures are investigated and a pattern is provided with the message exchange details. To be able to correctly assess the efficiency of this approach, a new mobility model is defined in the evaluation chapter of this thesis. Based on this model, the analysis has shown that our hybrid solution that combines IBC and the certified based security scheme results in a significant bandwidth improvement over the standard’s approach, PKMv2. This work is the first study that unites the advantages of both IBC and the certified-based security scheme for improved security while maintaining low overhead for WiMAX.M.S.Includes bibliographical referencesIncludes abstrac

    Search for di-Higgs production with the ATLAS detector

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    Local aromatase activity alterations in breast cancer tissues: A potential way of decision support for clinicians

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    Background and aims: It is becoming evident that local estrogen exposure is important in postmenopausal breast cancer patients. The microenvironment is established by breast stromal cells based on communication with tumor cells that is essential to cancer development, invasion, and metastasis. Here we investigated aromatase activity levels in both tumor and matched stromal tissues by showing their impact on the manufacturing of local estrogen and tumor progression in cases of invasive ductal carcinoma (IDC). Methods: Tumor (T) and tumor-associated stroma (TAS) neighboring tissues were acquired from each postmenopausal patient, diagnosed with IDC, and categorized as luminal A (n = 20). The control group was formed from tumor-free breast tissue samples (N, n = 12). A microsomal-based technique was created to compare breast tissue aromatase activities using liquid chromatography - mass spectrometry. Findings: We observed that the TAS tissues have the highest aromatase activities (p &lt; 0.05). High progesterone receptor (PR) intensity levels were found to be decreasing the activity level in these tissues significantly (p &lt; 0.05). Tumor tissue specific aromatase activity levels of postmenopausal patients&apos; were tend to be lower compared to healthy premenopausal subjects&apos; (3 fold, p &lt; 0.001). In addition low activity in tumor tissues were associated with low grade and late stage cancers. Conclusions: Early detection and personalized therapy is essential for postmenopausal breast cancer patients. Together, our in-house tandem mass spectrometry technique has the potential for further development and standardization for the measurement of aromatase activity and may assist clinicians decide on therapy policies for postmenopausal IDC patients which could be an invaluable asset for precise and specific evaluation

    Monitoring biodegradation capacity of organic pollutants in the environment

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    Micro-organismen zijn in staat om organische verbindingen om te zetten in minder schadelijke stoffen en spelen daarom een belangrijke rol bij het opruimen van milieuvervuiling. Voor beleidsmakers, landgebruikers en landeigenaren is het belangrijk dat er bij milieuverontreiniging goed toezicht wordt gehouden op de biologische afbraakprocessen en dat deze goed worden beheerst. Aangezien microbiële activiteit in het milieu wordt beïnvloed door diverse fysische, geochemische en biologische factoren, is nauwkeurige kennis van het afbraakproces hierbij noodzakelijk. In dit promotieonderzoek wordt de relatie tussen geochemische condities en de biologische afbraakcapaciteit van micro-organismen in het milieu beschreven, inclusief methoden om de activiteit en metabole functies van deze micro-organismen in het milieu te mete

    Experiments to study seismic drift of reinforced concrete structures

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    Earthquake-simulation tests of fifteen reinforced concrete single-degree-of-freedom systems were made to provide benchmark data for studying nonlinear drift response. Test variables included strength and initial period of the specimen, and frequency content and intensity of simulated earthquake motions.Analysis of measured response identified three tools for estimating seismic drift of structures. An idealized linear response spectrum for a damping ratio of 0.02 can be used to provide a quick check of overall drift or to compare structural alternatives. The substitute-structure method provides member design forces that are based on prescribed drift limits and control of damage. For nonlinear response history analysis using Takeda's hysteresis rules, values of five analytical parameters that gave the best results were identified.Made available in DSpace on 2011-05-07T13:57:15Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 8916215.pdf: 8925476 bytes, checksum: 483fca57a21695b90ee2c106a4db9e1b (MD5) Previous issue date: 1989Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding ([email protected]) on 2011-05-07T15:01:12Z Item is restricted indefinitely.Restriction data tranferred 2014-07-01T11:29:01-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permissionETDs are only available to UIUC Users without author permissionU of I Onl
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