11,972 research outputs found
Evolution-Guided Engineering of Elongation Factor-Tu for Incorporation of Non-Canonical Amino Acids
No full textSite-specific incorporation of non-canonical amino acids (ncAAs) has emerged as a promising tool for synthesizing proteins with novel biological properties. However, the breadth of ncAA incorporation systems is limited by incompatibility between ncAAs and the endogenous protein translation machinery. Specifically, Elongation Factor Tu (EF-Tu), a critical translational component that proofreads and delivers aminoacyl-tRNAs to the ribosome, has been a major barrier for ncAA incorporation. Here we employ reconstructing-evolutionary-adaptive-paths (REAP) analysis to create a site-saturation library based on 48 positions of EF-Tu that potentially modulate its recognition of ncAA-tRNA complexes. Using a novel in vivo antibiotic resistance-based selection assay, we isolate 29 EF-Tu variants can that incorporate p-azido-L-phenylalanine, p-benzoyl-L-phenylalanine or Nε-L-thiaprolyl-L-lysine via an amber codon. To further evaluate EF-Tu variants that demonstrated substrate-promiscuity in vivo, we constructed an in vitro assessment system based on the PURExpress system. With assistance from flexizyme, we examine the ability of EF-Tu variants to incorporate ncAAs that are incompatible with aminoacyl-tRNA synthetases. These in vitro assays allow us to interrogate EF-Tu variants for their individual abilities to incorporate ncAAs. Collectively, these findings support an evolutionary approach to identify poly-specific or ncAA-specific EF-Tu variants and broaden the scope of ncAA incorporation.Doctor of Philosophy (PhD)Biolog
Evaluating Research in Context: Pilot Study at Faculty of Architecture TU Delft
No full textThe vice chancellor of Delft University of Technology, Prof.dr.ir. J. Fokkema, introduced a pilot Evaluating Research in Context (ERiC) at the Faculty of Architecture. The Faculty of Architecture perceives a serious confl ict between the demands and criteria in evaluation procedures and the ambition to be relevant for the practice of architecture, planning and building. The goal of this ERiC pilot is to develop an evaluation method that judges research in the Faculty of Architecture on all its merits and takes into account the specifi c characteristics of architecture research. A second goal is the improvement of the evaluation of research contributions to society. This report presents the full results of this project and gives recommendations how to use these results in an evaluation according to the Standard Evaluation Protocol 2009-2015.Architectur
ROSENTHAL, Eric Inventory of documents
No full textCOVERAGE 1904; 1 File; 011 metre.Private papers of Eric Rosenthal, author, journalist and broadcaster
tritrophic-dispersal-model: Code used for creating figures for "Non-hierarchical dispersal promotes stability and resilience in a tri-trophic metacommunity"
No full text<p>This is the commented code used for creating figures for the paper. Any questions regarding the code should be directed to the corresponding author and repository owner (Eric Pedersen). </p>
Eric Velazquez Spanish Language Picture Book Award 2022 Acceptance Speech
No full textAuthor Eric Velazquez gives his Silver Medal acceptance speech for Pulpo Guisado (Holiday House)https://educate.bankstreet.edu/spanishlanguageaward/1001/thumbnail.jp
Eric C. Lincoln, Professor of Sociology and Religion, 1971
No full textThis is an interview with Eric C. Lincoln. Eric was a Professor of Sociology and religion, Union Theological Seminary and author of many books and articles on Negro history. In this recording the contributors discuss local memphis politics, sociology, and race relations compared to that of other cities in the South and the rest of the country
Interview with Eric Bentley, author, drama critic, and playwright
No full textDistinguished drama critic and Bertolt Brecht scholar, Eric Bentley is interviewed by WTMJ-TV host Jim Peck and John B. Fuegi, associate professor of Comparative Literature. Bentley recalls his association with Brecht, the critical and creative aspects of literature, and his interest in writing plays for the theater.GrayscaleSoun
Dr. Eric Yellin – Faculty Author Interview
No full textDr. Eric Yellin, Associate Professor of History and American Studies discusses his new book, Racism in the Nation’s Service: Government Workers and the Color Line in Woodrow Wilson’s America, published recently by the University of North Carolina Press. In this book, Dr. Yellin argues that President Wilson’s administration successfully segregated the federal government in the age of progressive politics. He investigates how the enactment of the segregation policy imposed a color line on American opportunity and implicated Washington in the economic limitation of African Americans for decades to com
Essentials of Dental Radiography and Radiology / Eric Whaites and Nicholas Drage.
No full textPrevious edition: Essentials of dental radiography and radiology / Eric Whaites. Fourth edition. Edinburgh ; New York : Churchill Livingstone, 2007.Includes bibliographical references (pages 461-464) and index.x, 478, [2] pages
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