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Posttranscriptional regulation of the components of the human leukocyte antigen class I antigen processing and presentation machinery as an immune escape mechanism in melanoma
Get PDFIm Rahmen der vorliegenden Arbeit wurden die Aspekte der posttranskriptionellen Regulation von HLA-I-APM-Komponenten, einschließlich der TAP-Untereinheiten TAP1 und TAP2 zusammen mit dem Chaperon TPN und HLA-I-Oberflächenexpressionsrate, durch miRs und RBPs analysiert. Basierend auf der erfolgreichen Identifizierung neuartiger miRs und RPBs durch die Durchführung von Hochdurchsatzanalysen in Kombination mit den miTRAP- und RNA-AP-Assays wurde der Schwerpunkt auf die Definition posttranskriptionaler Regulatoren von TAP1, TAP2 oder TPN gelegt, die auf die jeweiligen 3'-UTRs abzielen. Darüber hinaus wurde die spezifische Bindung der Kandidaten-miRs miR-200a-5p, miR-26b-5p und miR-21-3p an die TAP1 3'-UTR erfolgreich über Dual-Luciferase-Reporter-Assays bestätigt. Darüber hinaus wurde ihre Auswirkung auf die Anfälligkeit für eine Lyse von entweder CD8+ T- oder NK-Zellen bewertet. Eine Reihe von Kandidaten-RBPs wurde auch erfolgreich identifiziert, die an die 3'-UTRs von TAP1 bzw. TPN binden. Unter diesen wurde die Rolle der Kandidaten-RBPs IGF2BP1 und IGF2BP3 detaillierter charakterisiert. Diese Moleküle könnten daher als neuartige prädiktive Marker oder sogar als Ziele für zukünftige kombinatorische Krebsimmuntherapieansätze verwendet werden.In the context of the current thesis, the aspects of posttranscriptional regulations of HLA-I APM components, including the TAP subunits TAP1 and TAP2 along with the chaperone TPN next to the HLA-I surface expression rate, by miRs and RBPs were analysed in more detail in several in-house extensively characterized human melanoma cell lines. Based on the successful identification of novel miRs and RPBs by performing high throughput analyses in combination with the miTRAP and RNA-AP assays, respectively the focus was set on defining posttranscriptional regulators of TAP1, TAP2 or TPN, targeting the respective 3’-UTRs. Moreover, the specific binding of the candidate miRs miR-200a-5p, miR-26b-5p and miR-21-3p to the TAP1 3’-UTR have been successfully confirmed via dual luciferase reporter assays. Furthermore, their impact in regard to the susceptibility to undergo lysis be either CD8+ T- or NK cells were assessed. A set of candidate RBPs was also successfully identified which bind to either the 3’-UTRs of TAP1 or TPN, respectively. Amongst them, the roles of the candidate RBPs IGF2BP1 and IGF2BP3 have been characterized in more detail. These molecules might thus be used as novel predictive markers or even as targets for future combinatorial cancer immunotherapy approaches
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
CONTRIBUTION TO THE STUDY OF PROTHYMOSIN A AND PARATHYMOSIN A IN MAN
No full textOUR AIM WAS (A) THE DEVELOPMENT OF SENSITIVE AND SPECIFIC RADIOIMMUNOASSAYS FORA- AND B-THYMOSINS FOR THE MEASUREMENT OF THEIR LEVELS IN TISSUE EXTRACTS ANDBIOLOGICAL FLUIDS, (B) THE STUDY OF THEIR SUBCELLULAR DISTRIBUTION AND (C) THEMEASUREMENT OF THEIR LEVELS AND THE IDENTIFICATION OF THE CROSSREACTIVE MATERIAL FOR A- THYMOSINS IN SOLID TISSUE EXTRACTS AND IN BLOOD PLASMA IN HEALTH AND DISEASE. WE OBSERVED OF THE LEVELS OF BOTH POLYPEPTIDES WITH AGE. ON THE CONTRARY, IN MALIGNANT SOLID TISSUES (STATE INCLUDING INCREASED CELLULAR MULTIPLICATION) AND IN BLOOD PLASMA FROM AIDS PATIENTS (STATE CONNECTED WITH A HIGH DEGREE OF CELLULAR DESTRUCTION) THE LEVELS OF BOTH A-THYMOSINS WERE SIGNIFICANTLY HIGHER.ΣΚΟΠΟΣ ΗΤΑΝ (Α) Η ΑΝΑΠΤΥΞΗ ΕΙΔΙΚΩΝ ΚΑΙ ΕΥΑΙΣΘΗΤΩΝ ΡΑΔΙΟΑΝΟΣΟΔΟΚΙΜΑΣΙΩΝ ΤΩΝ Α- ΚΑΙ Β-ΘΥΜΟΣΙΝΩΝ ΓΙΑ ΤΗΝ ΑΝΙΧΝΕΥΣΗ ΚΑΙ ΤΗ ΜΕΤΡΗΣΗ ΤΩΝ ΕΠΙΠΕΔΩΝ ΤΩΝ ΠΟΛΥΠΕΠΤΙΔΙΩΝ ΣΕ ΕΚΧΥΛΙΣΜΑΤΑ ΙΣΤΩΝ ΚΑΙ ΣΕ ΣΩΜΑΤΙΚΑ ΥΓΡΑ, (Β) Η ΜΕΛΕΤΗ ΤΗΣ ΥΠΟΚΥΤΤΑΡΙΚΗΣ ΤΟΥ ΚΑΤΑΝΟΜΗΣ ΚΑΙ Γ) Η ΜΕΤΡΗΣΗ ΤΩΝ ΕΠΙΠΕΔΩΝ ΚΑΙ Η ΤΑΥΤΟΠΟΙΗΣΗ ΤΩΝ ΧΙΑΣΤΙ ΠΡΟΣ ΤΙΣ Α-ΘΥΜΟΣΙΝΕΣ ΑΝΤΙΔΡΩΝΤΩΝ ΥΛΙΚΩΝ ΣΕ ΕΚΧΥΛΙΣΜΑΤΑ ΣΥΜΠΑΓΩΝ ΙΣΤΩΝ ΚΑΙ ΣΤΟ ΠΛΑΣΜΑ ΤΟΥ ΑΙΜΑΤΟΣ ΣΤΗΝ ΥΓΕΙΑ ΚΑΙ ΣΕ ΑΣΘΕΝΕΙΣ. ΔΙΑΠΙΣΤΩΘΗΚΕ ΠΑΡΑΛΛΗΛΗ ΚΑΙ ΟΜΑΛΗ ΠΤΩΣΗ ΤΩΝ ΕΠΙΠΕΔΩΝ ΚΑΙ ΤΩΝ ΔΥΟ ΠΟΛΥΠΕΠΤΙΔΙΩΝ ΜΕ ΤΗΝ ΑΥΞΗΣΗ ΤΗΣ ΗΛΙΚΙΑΣ. ΑΝΤΙΘΕΤΑ, ΣΕ ΣΥΜΠΑΓΕΙΣ ΙΣΤΟΥΣ ΔΙΗΘΗΜΕΝΟΥΣ ΑΠΟ ΚΑΡΚΙΝΟ (ΚΑΤΑΣΤΑΣΗ ΠΟΥ ΣΥΝΕΠΑΓΕΤΑΙ ΑΥΞΗΜΕΝΟ ΚΥΤΤΑΡΙΚΟ ΠΟΛΛΑΠΛΑΣΙΑΣΜΟ) ΚΑΙ ΣΤΟ ΠΛΑΣΜΑ ΤΟΥ ΑΙΜΑΤΟΣ ΑΣΘΕΝΩΝ ΜΕ AIDS (ΚΑΤΑΣΤΑΣΗ ΠΟΥ ΣΥΝΕΠΑΓΕΤΑΙ ΑΥΞΗΜΕΝΗ ΚΥΤΤΑΡΙΚΗ ΚΑΤΑΣΤΡΟΦΗ) ΤΑ ΕΠΙΠΕΔΑ ΚΑΙ ΤΩΝ ΔΥΟ ΘΥΜΟΣΙΝΩΝ ΒΡΕΘΗΚΑΝ ΑΥΞΗΜΕΝΑ
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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