12,287 research outputs found
Disabled-2 Small Interfering Rna Modulates Cellular Adhesive Function and Mapk Activity during Megakaryocytic Differentiation of K562 Cells
No full textPrevious studies have shown that Disabled-2 (DAB2) is up- regulated during megakaryocytic differentiation of human K 562 cells. To delineate the consequences of DAB2 induction, a DNA vector-based small interfering RNA (siRNA) was designed to intervene in DAB2 expression. We found that DAB2 siRNA specifically inhibited DAB2 induction, resulting in the modulation of cell-cell adhesion and mitogen-activated protein kinase ( MAPK) phosphorylation. The morphological changes and 03 integrin expression associated with megakaryocytic differentiation were not affected. Since the MAPK pathway has been shown to involve DAB2 induction [Tseng et al., Biochem. Biophys. Res. Commun. 285 (2001) 129-135], our results suggest a reciprocal regulation between DAB2 and MAPK in the differentiation of K562 cells. In addition, we have demonstrated for the first time that DAB2 siRNA is a valuable tool for unveiling the biological consequences of DAB2 expression
Coherent selection of invisible high-order electromagnetic excitations
Full text linkResearch data for Coherent selection of invisible high-order electromagnetic excitations, Ming Lun Tseng, Xu Fang, Vassili Savinov, Pin Chieh Wu, Jun-Yu Ou, Nikolay I. Zheludev, and Din Ping Tsai, Scientific Reports 7, 44488(2017)</span
Quantitative Analysis of Multidrug-Resistance Mdr1 Gene Expression in Head and Neck Cancer by Real-Time Rt-Pcr
No full textProgression of head and neck cancer is always associated with changes of gene expression profile. In this study, we characterized the expression of multidrug-resistance mdr1 gene, which may play a role in tumorigenesis and multidrug resistance in head and neck cancer. A TaqMan one-step RT-PCR with a linear range for quantification across at least a 5 log scale of concentration of mdr1 mRNA was designed to determine the level of mdr1 expression in 50 pairs of normal vs. malignant head and neck tissues. Both the absolute level of mdr1 mRNA in tumor (T) and the relative mdr1 expression between tumor and its normal counterpart (T/N) were measured and their associations with several clinical variables were analyzed. Among the clinical variables analyzed, only the clinical stage of tumor was found to be associated with mdr1 expression. The distribution of clinical stages differed significantly (Pl, with 59.3%, 22.2%, 14.8% and 3. 7% in stage IV, III, II, and I, respectively. In addition, 76% of stage IV and 75% of stage III tumors had a T/N>1 compared to 25% of stage II and 20% of stage I tumors ( P=0. 004). Multivariate logistic regression analysis also indicated a significant difference of mdr1 expression between the early (I and II) and advanced (III and IV) stages tumors. The adjusted odds ratios(95% confidence intervals) were 1.477 (1.084-2.012) and 1.001 (1.000-1.002) for T/N (P<0.05) and T (P<0.05) treated as continuous variables, and 15.521 ( 3.414-70.550) and 5.074 (1.154-22.311 ) for T/N (P<0.001) and T (P<0.05) treated as binary variables, respectively. Taken together, the data presented here indicated that real-time RT-PCR provides a quantitative way to monitor mdr1 gene expression. The differential expression of mdr1 between early and advanced stages of head and neck cancer may shed light on the process of tumorigenicity and offer clues to the planning of new treatments
Lack of Association between Angiotensin-Converting Enzyme Gene Polymorphism and Peripheral Vascular Disease in Type 2 Diabetic Patients in Taiwan
No full textA total of 361 Chinese patients with type 2 diabetes were studied for the association between peripheral vascular disease (PVD) and the insertion/ deletion polymorphism involving a 287-bp alu repeat sequence at intron 16 of the angiotensin-converting enzyme (ACE) gene. The patients were divided into PVD (+) (n=45) and PVD (-) (n=316) based on an ankle-brachial index <0.9 and greater than or equal to0.9, respectively. Polymerase chain reaction was used to identify gene polymorphism. Results showed that D allele frequency in the patients without and with PVD was 31.8% and 33.3%, respectively (p=NS). The prevalence rates of I[, ID and DD genotypes in the PVD (-) group were 45.6%, 45.3% and 9.2%, respectively; and the respective values for the PVD (+) group were 44.4%, 44.4% and 11.1% (p=NS) . Prevalence rates of PVD in genotypes 11, ID, and DD were 12.2%, 12.3% and 14. 7%, respectively (p=NS). In logistic regression analyses, the unadjusted and adjusted odds ratios for DD vs II and ID vs R genotypes for PVD were not statistically significant. The respective adjusted odds ratios were 1.88 (0.56-6.29) and 1.33 (0.63-2.80). In conclusion, there was not a significant association between the ACE genotype and PVD in Chinese type 2 diabetic patients. However, a type 2 error can not be ruled out
Meeting the challenge? Transitions out of long-term homelessness
Full text linkJourney to Social Inclusion (J2SI) is a pilot project designed to break the cycle of long-term homelessness. The project provides intensive support for up to three years to assist people who are long-term homeless receive the range of services they need.
This is the second of four reports evaluating the J2SI project. It documents the social outcomes and the economic costs and benefits from the first 24 months. The evaluation uses a randomised controlled trial that tracks the outcomes of J2SI participants (Group J) and compares their outcomes with those of a comparison group (Group E) who are being supported by existing services.
After 24 months the evidence shows a sustained improvement in the housing circumstances of the J2SI participants compared to those in the control group. Critically, most (86%) have maintained their housing. While the move to independent housing was difficult in the beginning, the high rate of housing retention suggests that most of the participants are developing the skills and confidence needed to keep their housing.
The report contains the results of a cost-benefit analysis of the program after 2 years of operation.
The evaluation was conducted by researchers from RMIT University and the Melbourne Institute of Applied Economic and Social Research.
Authors: Guy Johnson, Daniel Kuehnle, Sharon Parkinson, Yi-Ping Tseng
Steinernema taiwanensis n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Taiwan
No full textTseng, Ching-Tzu, Hou, Roger F., Tang, Li-Cheng (2018): Steinernema taiwanensis n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Taiwan. Zootaxa 4434 (3): 466-480, DOI: 10.11646/zootaxa.4434.3.
Tseng, Wei Ching: I Ging und der Einfluss auf die Musik
No full textKünstlerische Masterarbeit Harfe
Lecture Recital vom 8.4.201
Blackfoot Disease in Taiwan: Its Link with Inorganic Arsenic Exposure from Drinking Water
No full textAging and Current Perception Threshold Measured by Neurometer in Normal Taiwanese Adults
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