1,720,964 research outputs found
Cytokinesis-block micronucleus assay in primary human liver fibroblasts exposed to griseofulvin and mitomycin C
No full textSusceptibility to chromosome malsegregation in lymphocytes of women who had a Down syndrome child in young age
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Spontaneous and induced chromosome damage in somatic cells of sporadic and familial Alzheimer's disease patients
No full textOxidative DNA damage in peripheral leukocytes of mild cognitive impairment and AD patients
No full textAbstract
It is well established that oxidative stress plays a key role in the degenerative neuronal death and progression of Alzheimer’s disease (AD),
although it is not clear if it is the primary triggering event in the pathogenesis of this disorder. Mild cognitive impairment (MCI) is a clinical
condition between normal aging and AD, characterized by a memory deficit without loss of general cognitive and functional abilities. We
performed this study by a comet assay analysis to evaluate the level of primary and oxidative DNA damage in two groups of MCI and AD
patients, compared to healthy controls. Data showed a significantly higher level of primary DNA damage in leukocytes of AD and also of MCI
patients compared to control individuals (average: 2.09±0.79 and 2.47±1.01, respectively for AD and MCI, versus 1.04±0.31 in controls).
Moreover, the amount of oxidised DNA bases (both purines and pyrimidines) was significatively higher in the two groups of patients (AD
and MCI) compared to controls. Our results give a further indication that oxidative stress, at least at the DNA level, is an earlier event in the
pathogenesis of AD
Susceptibility to chromosome malsegregation in lymphocytes of women who had a Down syndrome child in young age
No full textAbstract
Recent findings seem to converge towards a unified hypothesis trying to relate Down’s syndrome (DS), trisomy 21 and Alzheimer’s
disease (AD). The majority of DS individuals develop neuropathological characteristics of AD by the age of 40. Previous cytogenetic studies
performed by us showed an increased frequency of aneuploidy in peripheral lymphocytes and fibroblasts of AD patients and a preferential
occurrence of chromosome 21 in malsegregation events. An increased frequency of AD among young mothers of individuals with DS (MDS)
is reported. This study investigates the cytogenetic characteristics and the predisposition to chromosome malsegregation of peripheral blood
lymphocytes in a group of women (n = 35) who had a Down syndrome child in young age (<35 years) and in a control group (n = 30). We
applied the micronucleus assay and the dual-color FISH in order to assess the susceptibility to malsegregation events. The results indicate a
higher frequency of binucleated micronucleated cells in MDS in respect to the control group (16.1±9.1‰ versus 8.7±5.4‰). Moreover, our
data reveal that peripheral lymphocytes of MDS are more prone to chromosome non-disjunction with both chromosomes, 13 and 21, equally
involved
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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