1,720,990 research outputs found
Identification of a HLA-A*0201-restricted immunogenic epitope from the universal tumor antigen DEPDC1
Full text linkThe identification of universal tumor-specific antigens (TSA) shared between multiple patients and/or multiple tumors is of great importance to overcome the practical limitations of personalized cancer immunotherapy. Recent studies support the involvement of DEPDC1 in many aspects of cancer traits, such as cell proliferation, anti-apoptosis and cell invasion, suggesting that it may play key roles in the oncogenic process. In this study, we report that DEPDC1 expression is up-regulated in several types of human tumors, and closely linked to a poorer prognosis; therefore, it might be regarded as a novel universal oncoantigen potentially suitable for targeting many different cancers. In this regard, we report the identification of an immunogenic DEPDC1-derived epitope restricted for the HLA-A*0201 molecule, which is able to induce cytotoxic T lymphocytes (CTL) exerting a strong and specific functional response in vitro in response not only to peptide-loaded cells but also to triple negative breast cancer (TNBC) cells endogenously expressing the DEPDC1 protein. Such CTL are also therapeutically active against human TNBC xenografts in vivo upon adoptive transfer in immunodeficient mice. Overall, these data provide evidences that this DEPDC1-derived antigenic epitope can be exploited as a new tool for the development of immunotherapeutic strategies for HLA-A*0201 patients with TNBC, and potentially many other cancers. Moreover, we plan to employ an approach of multiplexing digital pathology to study the intimate relationships that adoptively transferred lymphocytes can establish with TNBC cells in tumor-bearing mice, as further advances in immunotherapy approaches require a detailed understanding of cell dynamics within the tumor microenvironment. The benefits of multispectral immunohistochemistry, combined with the development of software for quantitation, are making this methodology an increasingly powerful tool in the analysis and characterization of tissue and cellular processes, supporting diagnostic potential in order to improve therapies
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Thyroid-like metastases to the scalp from a papillary renal cell carcinoma: A case report
No full textThe skin can host metastatic tumors originating from different organs. We report a case of metastatic renal cell carcinoma to the scalp in a 73-year-old man with features very similar to those of thyroid papillary carcinoma. The histogenesis in relation to its structure is discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Il progetto di Paesaggio come modalità di lavoro nei contesti urbanizzati contemporanei
Full text linkNella tesi si tenta di argomentare come il progetto di paesaggio si sia costituito come una declinazione teorica complessa, capace di metabolizzare problemi e ipotesi di ricerca emergenti in una fase di profonde trasformazioni. Si tratta in particolare dei temi di progetto connessi alla crisi della città nelle diverse accezioni novecentesche, all’irrompere della questione ecologica attraverso la dimensione del rischio (minacce connesse all’aumento demografico, alle trasformazioni climatiche, alle grandi conurbazioni, ecc.), al riaffermarsi dell’azione diretta da parte delle comunità come risposta alla condizione di crisi e come pratica ineludibile nei processi di costruzione del paesaggio. L’ipotesi centrale della tesi è che queste differenti tensioni stiano trovando un punto di incontro, e di possibile co-evoluzione in chiave progettuale, grazie al feed back tra consapevolezza ecologica e nuove forme di partecipazione, particolarmente significativo nei progetti di paesaggio contemporanei. Questa relazione favorisce la messa a punto di nuove modalità di lavoro che sembrano scardina¬re le contrapposizioni e le antinomie ereditate dal recente passato e radicate negli immaginari collettivi e scientifici quali: artificiale/naturale, scientifico/ creativo, temporaneo/permanente, strategico/tattico. La ricerca muove da una rassegna di progetti ritenuti particolarmente significativi rispetto ai temi di riflessione teorica (nuovi stili di vita, infrastrutture complesse e forme di re¬silienza collaborativa), a cui segue un’indagine critica centrata su alcuni movimenti progettuali nordamericani, che hanno avuto un’influenza esplicita nelle formulazioni progettuali stesse e nella loro evoluzione. Si tratta del Landscape Urbanism e dell’Ecological Urbanism, particolarmente attivi nella riflessione sui mutamenti della città contemporanea, a cui segue una ricognizione dei movimenti di partecipazione dal basso, sempre di matrice nordamericana, riconducibili allo slogan Do-it-yourself Urbanism, significativi per la messa a fuoco di nuove modalità di lavoro di matrice collettiva. Grazie alle “ricognizione storico-critica” effettuata nei tre capitoli centrali è stato possibile evidenziare come molte riflessioni progettuali contemporanee, ormai completamente estranee ai rigidi confini settoriali del passato, stiano delineando nuove possibilità di ricomposizione e collaborazione tra termini antinomici radicati in un vasto immaginario progettuale novecentesco, ed in particolare le contrapposizioni tra scientifico/creativo, temporaneo/permanente, strategico/tattico
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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