1,721,064 research outputs found
Influenza di diverse auxine sulla rigenerazione da callo in pomodoro (Solanum lycopersicon L.)
No full textAuthentication of saffron (Crocus sativus L.) in different processed, retail products by means of SCAR markers
No full textA method based on Sequence-Characterized Amplified Regions (SCARs) was applied to 24 different food products composed by or containing different amounts of saffron (Crocus sativus L.), in order to detect adulteration/contamination with seven common bulking agents, namely Arnica montana L., Bixa orellana L., Calendula officinalis L., Carthamus tinctorius L., Crocus vernus L. (Hill), Curcuma longa L. and Hemerocallis sp. The food products screened included ground saffron, mixed seasonings and spices, ready-made dishes and herbal formulations. The method allowed a very good performance in both DNA extraction and amplification stages regardless of the presence of lipidic, sugary or polyphenolic substances and despite the presence of dried, stored and processed material. Two samples evidenced a contamination with C. vernus, four with Carthamus, one with Curcuma and one with Hemerocallis, while a product was found to contain no C. sativus. The recourse to SCAR markers may represent a fast, reliable and low-cost screening method for the authentication of a wide range of dried food products containing saffron. Despite the great attention paid by the food industry to the authentication of saffron, some common bulking agents can be present in retail foods, as the 21% of the screened samples was not composed by pure saffro
Caratterizzazione di un frammento di DNA corrispondente ad un mRNA specificamente espresso durante l'organogenesi in vitro in pomodoro.
No full textTU92, a putative MIP gene involved in the early phases of "in vitro" culture and differentially switched off by hormonal treatments.
No full textCaratterizzazione di un frammento di cDNA corrispondente ad un mRNA specificamente espresso in espianti di cotiledone di pomodoro durante la organogenesi somatica in vitro .
No full textDeterminazione del contenuto di auxina e di citochinina endogena in tessuti allevati in vitro attraverso HPLC ed espressione del gene pG5 in protoplasti di tabacco.
No full textIdentification of a putative tomato MIP gene differently expressed during the early phases of "in vitro" culture.
No full text
Quality Control of Saffron (Crocus sativus L.): Development of SCAR Markers for the Detection of Plant Adulterants Used as Bulking Agents.
No full textA method based on Sequence-Characterised Amplified Regions (SCARs) was developed from Random Amplified Polymorphic DNA markers (RAPDs) specific for Arnica montana L., Bixa orellana L., Calendula officinalis L., Carthamus tinctorius L., Crocus vernus L. (Hill), Curcuma longa L. and Hemerocallis sp., in order to detect these common bulking agents in commercial saffron (Crocus sativus). The method enabled the unequivocal detection of low amounts (up to 1%) of each adulterant, allowing the preemptive rejection of suspect samples. Its enforcement limits the number of samples to be subjected to further evaluation with pharmacognostic or phytochemical analyses, especially when multiple batches have to be evaluated in a short time. The dimension of the amplicons is suitable for the analysis of degraded DNA obtained from dried, stored, processed and finely ground commercial material. Proper SCAR markers may represent a fast, sensitive, reliable and low-cost screening method for the authentication of dried commercial saffron material
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