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Role of hypertrophic chondrocytes in the pathological remodeling of the osteochondral junction during osteoarthritis : involvement of SDF-1 in angiogenesis
No full textL’arthrose (OA) est le siège d’une réactivation du processus d’ossification endochondrale, caractérisé par une différenciation hypertrophique des chondrocytes, une minéralisation du cartilage, une angiogenèse ostéochondrale et un remodelage de l’os sous-chondral. L’angiogenèse ostéochondrale pourrait jouer un rôle central dans la progression de l’OA mais les acteurs moléculaires responsables de la vascularisation du cartilage sont encore méconnus. Nous pensons que la différenciation hypertrophique des chondrocytes joue un rôle moteur dans le remodelage de la jonction ostéochondrale et particulièrement dans l’angiogenèse ostéochondrale, grâce à une synthèse accrue de facteurs angiogéniques. Nous avons montré que les chondrocytes hypertrophiques présentaient une signature transcriptomique en faveur d’un rôle pro-angiogénique et avons confirmé ce potentiel à l’aide de différents tests fonctionnels d’angiogenèse in vitro (adhérence de cellules endothéliales aux matrices extracellulaires de chondrocytes, prolifération, migration et formation de tubules en réponse à une stimulation par des milieux conditionnés de chondrocytes). Une étude du sécrétome des chondrocytes a identifié le Stromal-Derived Factor 1 (SDF-1) comme étant potentiellement à l’origine des effets pro-angiogéniques des chondrocytes hypertrophiques. Nous avons montré que le blocage de l’axe SDF-1/CXCR4 par l’AMD3100 abolissait les effets des chondrocytes hypertrophiques sur la migration et la tubulogenèse. La différenciation des chondrocytes pré-hypertrophiques en chondrocytes hypertrophiques pourrait donc favoriser la vascularisation pathologique du cartilage, en produisant du SDF-1.Osteoarthritis (OA) is the site of a reactivation of the endochondral ossification process, characterised by hypertrophic chondrocyte differentiation, cartilage mineralisation, osteochondral angiogenesis and subchondral bone remodeling. Osteochondral angiogenesis may play a central role in the progression of OA, but the molecular actors responsible for cartilage vascularisation are still unknown. We believe that hypertrophic differentiation of chondrocytes plays a pivotal role in osteochondral junction remodeling and particularly in osteochondral angiogenesis, through increased synthesis of angiogenic factors. We showed that hypertrophic chondrocytes has a transcriptomic signature in favour of a pro-angiogenic role and confirmed this potential using different functional assays of angiogenesis in vitro (adhesion of endothelial cells to chondrocyte extracellular matrices, proliferation, migration and tube formation in response to stimulation by chondrocyte conditioned media). A study of the chondrocyte secretome identified Stromal-Derived Factor 1 (SDF-1) as a potential source of the pro-angiogenic effects of hypertrophic chondrocytes. We showed that blocking the SDF-1/CXCR4 axis by AMD3100 abolished the effects of hypertrophic chondrocytes on migration and tubulogenesis. Differentiation of pre-hypertrophic chondrocytes into hypertrophic chondrocytes could therefore promote pathological vascularisation of cartilage by producing SDF-1
Rôle des chondrocytes hypertrophiques dans le remodelage pathologique de la jonction ostéochondrale au cours de l'arthrose : implication du SDF-1 dans l'angiogenèse
No full textOsteoarthritis (OA) is the site of a reactivation of the endochondral ossification process, characterised by hypertrophic chondrocyte differentiation, cartilage mineralisation, osteochondral angiogenesis and subchondral bone remodeling. Osteochondral angiogenesis may play a central role in the progression of OA, but the molecular actors responsible for cartilage vascularisation are still unknown. We believe that hypertrophic differentiation of chondrocytes plays a pivotal role in osteochondral junction remodeling and particularly in osteochondral angiogenesis, through increased synthesis of angiogenic factors. We showed that hypertrophic chondrocytes has a transcriptomic signature in favour of a pro-angiogenic role and confirmed this potential using different functional assays of angiogenesis in vitro (adhesion of endothelial cells to chondrocyte extracellular matrices, proliferation, migration and tube formation in response to stimulation by chondrocyte conditioned media). A study of the chondrocyte secretome identified Stromal-Derived Factor 1 (SDF-1) as a potential source of the pro-angiogenic effects of hypertrophic chondrocytes. We showed that blocking the SDF-1/CXCR4 axis by AMD3100 abolished the effects of hypertrophic chondrocytes on migration and tubulogenesis. Differentiation of pre-hypertrophic chondrocytes into hypertrophic chondrocytes could therefore promote pathological vascularisation of cartilage by producing SDF-1.L’arthrose (OA) est le siège d’une réactivation du processus d’ossification endochondrale, caractérisé par une différenciation hypertrophique des chondrocytes, une minéralisation du cartilage, une angiogenèse ostéochondrale et un remodelage de l’os sous-chondral. L’angiogenèse ostéochondrale pourrait jouer un rôle central dans la progression de l’OA mais les acteurs moléculaires responsables de la vascularisation du cartilage sont encore méconnus. Nous pensons que la différenciation hypertrophique des chondrocytes joue un rôle moteur dans le remodelage de la jonction ostéochondrale et particulièrement dans l’angiogenèse ostéochondrale, grâce à une synthèse accrue de facteurs angiogéniques. Nous avons montré que les chondrocytes hypertrophiques présentaient une signature transcriptomique en faveur d’un rôle pro-angiogénique et avons confirmé ce potentiel à l’aide de différents tests fonctionnels d’angiogenèse in vitro (adhérence de cellules endothéliales aux matrices extracellulaires de chondrocytes, prolifération, migration et formation de tubules en réponse à une stimulation par des milieux conditionnés de chondrocytes). Une étude du sécrétome des chondrocytes a identifié le Stromal-Derived Factor 1 (SDF-1) comme étant potentiellement à l’origine des effets pro-angiogéniques des chondrocytes hypertrophiques. Nous avons montré que le blocage de l’axe SDF-1/CXCR4 par l’AMD3100 abolissait les effets des chondrocytes hypertrophiques sur la migration et la tubulogenèse. La différenciation des chondrocytes pré-hypertrophiques en chondrocytes hypertrophiques pourrait donc favoriser la vascularisation pathologique du cartilage, en produisant du SDF-1
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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