8,078 research outputs found

    Références sur "Garibong-dong"

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    Liste établie par Eunjoo Carré-Na, le 6 mars 2012 Title/Author/Year 재중동포의 상업 활동과 정체성 형성 : 가리봉동 현장 연구를 중심으로 / 이민주  ( [2008]) 외국인 밀집거주로 인한 내국인 주민의 주거환경 인식에 관한 연구 : 한남동, 가리봉동을 중심으로 = Local Residents' Perception of Foreigner's Dwelling : A case study of Hannam-dong, Garibong-Dong in Seoul / 박신영  ( [2009]) 가리봉동 중국거리에서의 조선족 여성의 위치성에 대한 문화․지리적 연구 = A Cutural-Geological Study on Positionality of Korean-Chinese women in the China street at Garibong-dong / 이미애  ( [2008]) 한국의 외국인 ..

    Acoustic radiation due to scattering of T-S wave by the mean-flow distortion induced by steady local suction

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    Substantial sound waves can be generated by boundary-layer instability modes when the latter are scattered by a rapid mean-flow distortion. This is a rather generic mechanism and operates when an oncoming T-S wave is scattered by a steady local suction slot. This paper focuses on this problem by extending a recently developed Local Scattering Theory (Wu & Dong, J. Fluid Mech. submitted), where a so-called transmission coefficient, defined as the ratio of the T-S wave amplitude downstream of the scatter to that upstream, is introduced to characterize the effect of a local scatter on boundary-layer instability and transition. As in the earlier work, the mathematical formulation is based on triple-deck formulism, but in order to accommodate the acoustic far field, which was not considered in the paper mentioned, the unsteady terms in the upper deck, which play a leading-order role in radiation, are retained, and the influence of the radiated sound on the near-wall perturbation is included. The upper deck equation for the pressure is the Helmholtz equation rather than the Laplace equation. This leads to a modified pressure-displacement relation, which is coupled with the linearized boundary-layer equations in the lower deck. Discretization of the whole system formulates a generalized eigenvalue problem, which is solved numerically. It is found that suction suppresses oncoming T-S waves, and this effect increases with the suction velocity and the slot width. The directivity is ndependent of the flow parameters only when the Mach number is low. The intensity of the radiated sound in general increases with the frequency, the suction velocity and the width of the suction slot. Interestingly, for O(1) suction velocities, the radiated sound is very weak, indicating that the gain of stabilizing effect does not cause aeroacoustic penalty

    Erratum: 3D bioprinted in vitro secondary hyperoxaluria model by mimicking intestinal-oxalatemalabsorption-related kidney stone disease (Applied Physics Reviews (2022) 9 (041408) DOI: 10.1063/5.0087345)

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    © 2023 Author(s).This article was originally published online on 21 November 2022 with an incorrect affiliation identifier for author Dong-Woo Cho. It is correct as it appears above. All online versions of this article were corrected on 23 November 2022. AIP Publishing apologizes for this error.11Nsciescopu

    More images that yet fresh images beget

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    In this issue of Immunity, Dong et al. (2009) describe the protein crystal structure heterodimer of tapasin and ERp57, which helps visualize the function of these proteins in loading of peptide antigens onto MHC class I molecules as part of the peptide loading complex within the endoplasmic reticulum.<br/

    The Dong Oral Architecture: Carpenter, architecture and phenomena among the Dong people in southwest of China

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    The Dong is a minority mainly living in southwest China. The Dong people do not have written language, the dissemination of knowledge mainly relies on the oral education and practice, forming a unique process and method of oral education, of architectural construction and the use of architecture. In this thesis these three processes are linked together and understood to produce ‘Oral Architecture’. Oral architecture is a process through which the Dong architectural activity is reproduced and passed down through generations, letting people participate and observe phenomena, and thus apprehend the meaning of things and community. It is built on the relationships between people, activity and building. The series of activities that relate to buildings are simultaneously the motivation to construct intra-community relationships, to maintain traditions, and promote the broader process of living closely within their particular environment. Through field research, interviews, literature review, case studies and other methods, I have collected information about the process and methods of the Dong oral education, of architectural construction, and the use and meaning of their architecture. Informed by architectural phenomenology, the thesis offers a qualitative analysis of this data in order to summarise and understand the mode and concept of Dong oral architecture. The structure of the thesis provides a broad introduction to Dong society and culture, before analysing the education and practice of Dong Carpentry; the construction of the Dong House and the Drum Tower (the most important public building in any Dong village). Concluding chapters focus on how systems of meaning and ‘reading’ are supported by Dong building and their practices of everyday life, as well as the significant events of birth, marriage and death. All translations from Chinese are by the author unless otherwise stated

    Xi jun ji qun yun dong zhong shui dong xiang hu zuo yong de yan jiu

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    Zhai, He = 细菌集群运动中水动相互作用的研究 / 翟翯.Thesis M.Phil. Chinese University of Hong Kong 2015.Includes bibliographical references (leaves 54-59).Abstracts also in Chinese.Title from PDF title page (viewed on 08, November, 2016).Zhai, He = Xi jun ji qun yun dong zhong shui dong xiang hu zuo yong de yan jiu / Zhai He

    Building cultural and spiritual life of dong nai workers – real situation and solutions

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    Building cultural and spiritual life of Dong Nai workers is the process of equipping, training and upgrading the necessary qualities and competencies of the modern worker – the prerequisite that ensures the success of the innovation, industrialization and modernization in this region. On the basis of clarifying the basic characteristics of cultural and spiritual life of Dong Nai workers, the author analyzes the achievements and limitations in the construction of cultural and spiritual life of the workers in Dong Nai in recent years. The author proposes some main solutions to improving the cultural and spiritual life of Dong Nai workers for achieving the local economic and social goals in the current period

    Shin Dong Chul

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    학위논문(석사)--아주대학교 일반대학원 :의생명학과,2009. 2TABLE OF CONTENTS ABSTRACT &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; i TABLE OF CONTENTS &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; ii LIST OF FIGURES &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; iv I. INTRODUCTION &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 1 A. Function of mast cells &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 1 B. T cell immunoglobulin mucin domain 3 &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 2 C. The role of TGF-b in mast cells &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 3 D. Mitogen-activated protein kinase-dependent, smad-indefendent, signal pathway by TGF-b&#61472;&#61472;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 3 E. The role of GATA transcription factors in mast cells &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 4 F. Purposes of this research &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;5 II. MATERIALS AND METHODS &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 6 A. MATERIALS &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 7 B. METHODS &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 7 1. Cell culture &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 7 2. Total RNA preparation and cDNA synthesis &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 7 3. Semi-quantitative reverse transcription &#8211; polymerase chain reaction &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 8 4. Real-time PCR &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 9 5. Reverse transcription- polymerase chain reaction (RT-PCR) &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 10 6. IgE sensitization and Intracellular Ca2+ mobilization &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 11 7. Transfection of plasmids &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 11 8. SDS-PAGE and Western blotting &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 12 9. Statistical Analysis &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 13 III. RESULTS &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 14 A. TIM-3 transcription was differently regulated in HMC-1 cells and THP-1 cells &#8226; &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 14 B. TIM-3 transcription was not affected by LPS stimulationin HMC-1 cells &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 17 C. TIM-3 transcription was not changed by FceR stimulation in HMC-1 cells &#8226;&#8226;&#8226; 19 D. TGF-b did not change expression of TIM-3 ligand, galectin-9 in HMC-1 cells &#8226;&#8226;&#8226;21 E. TIM-3 transcription was regulated through ERK MAP kinase pathway in TGF-b&#61538; stimulated HMC-1 cells &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 22 F. GATA-1 transcription factor did not affect TIM-3 transcription in HMC-1 cells &#8226;&#8226;&#8226; 24 IV. DISCUSSION &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 26 V. CONCLUSIONS &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 30 REFERENCES &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 31 국문요약 &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 39 LIST OF FIGURES Fig. 1. TIM-3 transcription was increased in HMC-1 cells stimulated with TGF-b and in THP-1 cells stimulated with LPS respectively &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 15 Fig. 2. High concentration of LPS did not affect TIM-3 transcription level in HMC-1 cell &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 18 Fig. 3. TIM-3 transcription was not increased via Fce receptor signal in HMC-1 cells &#8226;&#8226; 21 Fig. 4. Transcription of galectin-9, a TIM-3 ligand, was not induced by TGF-b treatment. &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 22 Fig. 5. The increased TIM-3 transcription induced by TGF-b was attenuated by inhibition of ERK MAP kinase &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 24 Fig. 6. TIM-3 transcription was not upregulated by GATA-1 transcription factor &#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226;&#8226; 26MasterT cell immunoglobulin mucin domain 3 (TIM-3) which is a type-I membrane protein, is expressed on differentiated Th1 cell, mast cell and monocyte. Although expression of TIM-3 and its ligand galectin-9 has been demonstrated in mast cells, the transcriptional regulation of these molecules has not been well explored. Considering the emerging role of mast cells in host defense, I studied the effect of various cytokines, a TLR agonist or FcεR stimulation on transcription levels of TIM-3 and galectin-9 in a human mast cell line, HMC-1 cells. TIM-3 transcription in HMC-1 cell was upregulated only by TGF-b but not by IFN-a, IFN-l, TNF-a, IL-10, LPS or FcεR cross-linking. Whereas transcription of TIM-3 ligand, galectin-9 was not affected by treatment with TGF-b. To reveal downstream pathway of TGF-b stimulation involved in TIM-3 upregulation in HMC-1 cells, I examined MAPK pathway and the role of GATA-1. I demonstrated that TGF-b mediated TIM-3 upregulation was inhibited both by a inhibitor of ERK but not by inhibitors of p38 and JNK. Further, overexpression of GATA-1 did not affect TIM-3 expression in HMC-1 cells. These results suggest that TIM-3 transcription in mast cells may be upregulated by TGF-b through ERK MAP kinase signal

    1 - Samuel Dong Saul

    No full text
    This is a lightbox that is 40 x 25 inches in size, with different color lithographs on Mylar.Is it really strange to think that objects speak? Take a moment to think about this very closely; they might not be speaking to you in a verbal form, but they are surely communicating messages to you in way only you can understand. Could this means they could feel, think, just the way we do? Perhaps. Objects are often part of language, sometimes even before they exist. These objects become social or cultural artifacts well before they are used by living things. Objects "meanings" and "functions" are framed within the constrains of a cultural group. We assign them significance because of what they do, and what they are made off. Shapes, colors, matter, and the combinations of the three build pretty much everything in our observable universe. We just interpret what they might be. If we are made of the same matter, why not think that they might be "alive" too, with feelings and ideas of their own. We will never know what they really want, but we can sure interpret what they are trying to communicate. Contemporary artist make works that engage with viewers about theories of symbolic communication and strategies of analysis. Many of them use text simply for its ability to communicate meaning that are difficult to express in images alone; to convey ideas, descriptions, arguments, and possibly, the interpretation of what the objects someone interacts with, are trying to say.Visual and Performing Arts

    Hybrid carbon thermal interface materials for thermoelectric generator devices

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    Thermal interface materials (TIMs) are extensively used in electronic devices as efficient heat transfer materials. We fabricated all-carbon TIMs by hybridizing single-wall carbon nanotubes (SWCNTs) with graphite and demonstrated their performance by applying them to a thermoelectric generator (TEG) device. The hybrid carbon TIM exhibited maximum thermal conductivity when the SWCNT content was near 10 wt%. The TIM thermal contact resistance measured by a home-made calorimeter setup was 2.19 × 10−4m2K/W, which did not vary with temperature but decreased with applied pressure. Post-treatment of the TIM with a silane coupling agent further reduced the TIM thermal contact resistance by 30%. When the TIM was placed between a TEG device and a copper heat reservoir, the TEG output power increased with the temperature difference across the TEG and applied pressure. Moreover, the post-treatment of the TIM enhanced the output power of the TEG device by up to 18.5%. This work provides a simple and effective pathway towards a carbon-based TIM that can be applied to a high temperature TEG. © 2020, The Author(s).1
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