8 research outputs found
New continuous production process for enantiopure (2R,5R)-hexanediol
No full textA new continuous production process has been developed for optically active pure (2R,5R)-hexanediol. The process uses resting whole cells of Lactobacillus kefir DSM 20587 as a biocatalyst. The reduction of (2,5)-hexanedione to (2R,5R)hexanediol was carried out in a 2-L continuously operated membrane reactor. Conversion of (2,5)-hexanedione was nearly quantitative and the selectivity between product and intermediate was 78% for the product. Enantioselectivity and diastereoselectivity were >99% for the whole period. The productivity of L. kefir could be increased by factor 30. (2R,5R)-Hexanediol was continuously produced over 5 days with a space-time yield of 64 g.L-1.d(-1)
Effect of additives on gas-phase catalysis with immobilised Thermoanaerobacter species alcohol dehydrogenase (ADH T)
No full text
Optimization of Enzymatic Gas-Phase Reactions by Increasing the Long-Term Stability of the Catalyst
No full textEnzymatic gas-phase reactions are usually performed in continuous reactors, and thus very stable and active catalysts are required to perform such transformations on cost-effective levels. The present work is concerned with the reduction of gaseous acetophenone to enantiomerically pure (R)-1-phenylethanol catalyzed by solid alcohol dehydrogenase from Lactobacillus brevis (LBADH), immobilized onto glass beads. Initially, the catalyst preparation displayed a half-life of 1 day under reaction conditions at 40 °C and at a water activity of 0.5. It was shown that the observed decrease in activity is due to a degradation of the enzyme itself (LBADH) and not of the co-immobilized cofactor NADP. By the addition of sucrose to the cell extract before immobilization of the enzyme, the half-life of the catalyst preparation (at 40 °C) was increased 40 times. The stabilized catalyst preparation was employed in a continuous gas-phase reactor at different temperatures (25-60 °C). At 50 °C, a space-time yield of 107 g/L/d was achieved within the first 80 h of continuous reaction.
Heterogeneous Biocatalysis In Solid/gas Phase: Principles And Applications [biocatélise Heterogênea Em Fase Sólido/gés: Princípios E Aplicações]
No full textEnzymatic conversion of gaseous substrates into products in aquo-restricted media, using enzymes or whole cells (free and immobilized) as biocatalysts, constitutes a promising technology for the development of clearer processes. Solid-gas systems offer high production rates for minimal plant sizes, allow important reduction of treated volumes, and permit simplified downstream processes. In this review article, principles and applications of solid-gas biocatalysis are discussed. Comparisons of its advantages and disadvantages with those of the organic- and aqueous-phase reactions are also presented herein.372323330Lima, A.W.O., Angnes, L., (1999) Quim Nova, 22, p. 229Krishna, S.H., (2002) Biotechnol. Adv., 20, p. 239Illanes, A., Cauerhff, A., Wilson, L., Castro Guillermo, R., (2012) Bioresour. Technol., 115, p. 48Matsuda, T., (2013) J. Biosci. Bioeng., 115, p. 233Itabaiana Jr., I., Miranda, L.S.M., De Souza, R.O.M.A., (2013) J. Mol. Catal. 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Optimization of Adsorptive Immobilization of Alcohol Dehydrogenases
No full textIn this work, a systematic examination of various parameters of adsorptive immobilization of alcohol dehydrogenases (ADHs) on solid support is performed and the impact of these parameters on immobilization efficiency is studied. Depending on the source of the enzymes, these parameters differently influence the immobilization efficiency, expressed in terms of residual activity and protein loading. Residual activity of 79% was achieved with ADH from bakers’ yeast (YADH) after optimizing the immobilization parameters. A step-wise drying process has been found to be more effective than one-step drying. A hypothesis of deactivation through bubble nucleation during drying of the enzyme/glass bead suspension at low drying pressure (<45 kPa) is experimentally verified. In the case of ADH from Lactobacillus brevis (LBADH), >300% residual activity was found after drying. Hyperactivation of the enzyme is probably caused by structural changes in the enzyme molecule during the drying process. ADH from Thermoanaerobacter species (ADH T) is found to be stable under drying conditions (>15 kPa) in contrast to LBADH and YADH.
Laboratory evolution of Pyrococcus furiosus alcohol dehydrogenase to improve the production of (2S,5S)-hexanediol at moderate temperatures
No full textThere is considerable interest in the use of enantioselective alcohol dehydrogenases for the production of enantio- and diastereomerically pure diols, which are important building blocks for pharmaceuticals, agrochemicals and fine chemicals. Due to the need for a stable alcohol dehydrogenase with activity at low-temperature process conditions (30°C) for the production of (2S,5S)-hexanediol, we have improved an alcohol dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus (AdhA). A stable S-selective alcohol dehydrogenase with increased activity at 30°C on the substrate 2,5-hexanedione was generated by laboratory evolution on the thermostable alcohol dehydrogenase AdhA. One round of error-prone PCR and screening of ~1,500 mutants was performed. The maximum specific activity of the best performing mutant with 2,5-hexanedione at 30°C was tenfold higher compared to the activity of the wild-type enzyme. A 3D-model of AdhA revealed that this mutant has one mutation in the well-conserved NADP(H)-binding site (R11L), and a second mutation (A180V) near the catalytic and highly conserved threonine at position 183
