1,720,982 research outputs found
P-affinity measurements of specific osmotroph populations using cell-sorting flow cytometry
No full textTo elucidate the role that the marine microbes play in global nutrient cycling, it is necessary to recognize
how various phyto- and bacterioplankton groups compete for limiting nutrients. Specific phosphate affinity
describes an organism’s ability to harvest phosphate at low concentrations from the surrounding water. For the
first time, we have taken advantage of cell-sorting flow cytometry in combination with radio-labeled phosphorus
to measure this feature of specific osmotrophic groups in natural communities. Specific phosphate affinities
for Synechococcus spp. and picoeukaryotes were measured using live, unstained cells. The results were always
lower than theoretical calculated maximum values, corresponding well with observations of P-deficiency, or
sub-optimal P supply for the osmotroph community, at the time of investigation. Fixing and staining cells
before flow sorting offers the advantage of better separation of phytoplankton and showed high sorting reproducibility
when applied to nonaxenic Synechococcuscultures. A subsequent investigation of P-leakage from isotopically
labeled, fixed, stained cells in nonaxenic cultures of Synechococcusshowed that it was only slightly larger
than the loss of 17% found when uptake of new label was stopped with adding “cold” phosphate. Possible
applications of the currently developed methodology for population specific P affinity measurements by flow
sorting are discussed
How trophic cascades and photic zone nutrient content interact to generate basin-scale differences in the microbial food web
No full textIn linear food chains, resource and predator control produce positive and negative correlations, respectively, between biomass at adjacent trophic levels. These simple relationships become more complex in food webs that contain alternative food chains of unequal lengths. We have used a “minimum” model for the microbial part of the pelagic food web that has three such food chains connecting free mineral nutrients to copepods: via diatoms, autotrophic flagellates, and heterotrophic bacteria. Trophic cascades from copepods strongly modulates the balance between the three pathways and, therefore, the functionality of the microbial food web in services such as food production for higher trophic levels, DOM degradation, and ocean carbon sequestration. The result is a theoretical framework able to explain, not only apparent conflicts in Arctic mesocosm experiments, but also biogeochemical features of the Mediterranean. Here, the fundamental difference between Arctic and Mediterranean microbial food webs is the way they are predator driven by seasonal migration of large copepods in the Arctic, but resource driven due to the anti-estuarine circulation in the Mediterranean. In this framework, global change effects on microbial ecosystem functions are more like to come indirectly through changes in these drivers than through direct temperature effects on the microbes
Are bacteria active in the cold pelagic ecosystem of the Barents Sea?
No full textBacterial biomass and activity indicators have been studied at low water temperatures (?1.9 to +4°C) in Barents Sea. Strong responses by indicators of bacterial activity, such as hydrolytic enzyme and substrate uptake potentials, were observed in association with the development of phytoplankton blooms. At late successional stages of blooms, observation by epifluorescence microscopy revealed heavy bacterial colonisation of detrital matter, in particular of senescent colonies of Phaeocystis pouchetii. Based on the retention of bacteria on filters of 1 ?m pore size, up to 55% of the bacterial population was estimated to be attached to organic aggregates in some cases. Based on thymidine incorporation and a conventional conversion factor, bacterial generation times as short as one day were estimated at temperatures below zero. Changes in substrate availability governed by the successional stages of the planktonic ecosystem seem to be more important as controlling factors for bacterial growth than the low temperatures of the Barents Sea
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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