937 research outputs found
The use of high-affinity polyhistidine binders as masking probes for the selection of an NDM-1 specific aptamer
No full textThe emergence of carbapenemase-producing multi-drug resistant Enterobacteriaceae poses a dramatic, world-wide health risk. Limited treatment options and a lack of easy-to-use methods for the detection of infections with multi-drug resistant bacteria leave the health-care system with a fast-growing challenge. Aptamers are single stranded DNA or RNA molecules that bind to their targets with high affinity and specificity and can therefore serve as outstanding detection probes. However, an effective aptamer selection process is often hampered by non-specific binding. When selections are carried out against recombinant proteins, purification tags (e.g. polyhistidine) serve as attractive side targets, which may impede protein target binding. In this study, aptamer selection was carried out against N-terminally hexa-histidine tagged New Delhi metallo-ß-lactamase 1. After 14 selection rounds binding to polyhistidine was detected rather than to New Delhi metallo-ß-lactamase 1. Hence, the selection strategy was changed. As one aptamer candidate showed remarkable binding affinity to polyhistidine, it was used as a masking probe and selection was restarted from selection round 10. Finally, after three consecutive selection rounds, an aptamer with specific binding properties to New Delhi metallo-ß-lactamase 1 was identified. This aptamer may serve as a much-needed detection probe for New Delhi metallo-ß-lactamase 1 expressing Enterobacteriaceae
Babel, or the local distortion of the Greek language
No full textTitle: Βαβυλωνία ἤ ἡ κατά τόπους διαφθορά τῆς ἑλληνικῆς γλώσσας. Κωµωδία (Babel, or the local distortion of the Greek language: A comedy) Originally published: Ναύπλιο (Nafplio), Τυπογραφεῖsο Κωνσταντίνου Τόµπρα ἐκ Κυδωνιῶν καὶ Κωνσταντίνου Ἰωαννίδη ἐκ Σµύρνης, 1836 Language: Greek The excerpt used is from D. C. Vyzantios, Βαβυλωνία, introduction by Spyros Evangelatos (Athens: Εστία, 1993), pp.1–3. About the author Dimitrios Vyzantios (pseudonym of Dimitrios K. Hatziaslanis) [1790, Constantin..
Dimitrios Tsamis Karatasos : a symbol of Greek, Serbian and Bulgarian friendship
No full textTwo Serbian texts extol the contribution of Dimitrios Tsamis Karatasos to the Balkan joint effort to throw off the Ottoman yoke. These texts are analysed by the author within the historical framework of their period, so that the man’s personality and work may be accurately evaluated from a fresh viewpoint. More specifically, the author conducts a research on the tombstone of Dimitrios Tsamis Karatasos, which he discovered himself at Naoussa, and the octet engraved on it, which is also published here. The work is illustrated by seven plates, of which four have not been published previously
The Remains of authoritarianism : bureaucracy and civil society in post-authoritarian Greece
No full textDimitrios A. Sotiropoulos. 30 cm. He presented this paper at a seminar held at the Center on October 21, 1994. - T.p. Includes bibliographical references (p. 31-35
Erratum: Korkovelos, A., et al. The role of open access data in geospatial electrification planning and the achievement of SDG7. An OnSSET-based case study for Malawi. <i>Energies</i> 2019, 12(7), 1395
No full textThe authors wish to make a change in author names (adding new author—Dimitrios Mentis) to this paper
The Exemplary Life of Dimitrios Vikelas (1835-1908)
No full textThis paper describes the "satisfying curve" of Dimitrios Vikelas' life journey, starting from Syros in 1835, moving via Constantinople, Odessa, and Syros again, to London, Paris and finally Athens. It explores Vikelas' multiple aspects, as merchant, writer, traveller, lecturer and essayist, Olympic founder, educationalist, book collector and philanthropist, all of which were united in the public-spirited man of letters (logios). It sets Vikelas in the context of the Greek commercial diaspora, the world of the London expatriate Greek community, and the dynamic society of late nineteenth-century Athens, beginning in the 1870s to act as a magnet to Greek expatriates. The author stresses two qualities of Vikelas: his belief in the idea of a progressive Greek state marked by advances in education, culture, tourism and standards of public life; and the self-awareness and experience which inform his autobiographical writings, not only his memoir My Life but also his last such work, The War of 1897
Turbulence structure and flow resistance in oscillatory boundary layer flows
No full textSubmission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2022-12-01The student, Dimitrios Fytanidis, accepted the attached license on 2020-12-01 at 15:14.The student, Dimitrios Fytanidis, submitted this Dissertation for approval on 2020-12-01 at 15:22.This Dissertation was approved for publication on 2020-12-03 at 08:17.DSpace SAF Submission Ingestion Package generated from Vireo submission #16014 on 2021-03-04 at 16:32:49Made available in DSpace on 2021-03-05T21:45:43Z (GMT). No. of bitstreams: 15
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Previous issue date: 2020-12-03Embargo set by: Seth Robbins for item 117320
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Reason: Author requested closed access (OA after 2yrs) in Vireo ETD systemEmbargo set by: Seth Robbins for item 117320
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Reason: Author requested closed access (OA after 2yrs) in Vireo ETD systemAuthor requested closed access (OA after 2yrs) in Vireo ETD systemLimite
Correction: The effect of COVID rehabilitation for ongoing symptoms Post HOSPitalisation with COVID-19 (PHOSP-R): protocol for a randomised parallel group controlled trial on behalf of the PHOSP consortium (Trials, (2023), 24, 1, (61), 10.1186/s13063-023-07093-7)
No full text\ua9 2023, The Author(s).Following publication of the original article [1], it was noticed that the author name Dimitrios Megaritis was incorrectly written as Dimitrios Magaritis. The author group has been updated above and the original article has been corrected
Sachbericht zum Verwendungsnachweis
No full textDas (Wieder-) Auftreten viraler Infektionskrankheiten stellt eine ernsthafte Bedrohung für die globale öffentliche Gesundheit dar. Immer wieder verzeichnet die WHO durch Viren verursachte Epidemien oder Pandemien, beispielsweise SARS (2002-2003), MERS (2012) und COVID-19 (seit 2019). Virusinfektionen sind schwierig zu behandeln, weisen mitunter schwere Verläufe auf, die sogar zum Tod des Patienten führen können, und bedeuten somit auch eine enorme finanzielle Belastung für den Gesundheitssektor. Es besteht daher der dringende Bedarf, pathogene Viren frühzeitig zu erkennen und zu identifizieren, sowie ihr (Wieder-) Auftreten in der Umwelt und Bevölkerung zu überwachen.
Aktuell basiert die Identifizierung pathogener Viren auf der Analyse ihres Erbgutes, ihrer Morphologie und den spezifischen Krankheitssymptomen der Patienten. Die Untersuchung des genetischen Materials erfolgt dabei mittels Polymerase-Kettenreaktion (PCR) für DNA-Viren bzw. Reverse Transkriptase-PCR (RT-PCR) für RNA-Viren. Eine PCR ist jedoch zeitaufwendig, erfordert eine umfangreiche Probenvorbereitung und hochqualifiziertes Laborpersonal. Zudem werden eine ausgeklügelte Infrastruktur sowie teure Laborgeräte benötigt, die besonders Entwicklungsländer vor finanzielle Hürden stellen.
In diesem Verbundprojekt wurde die Erarbeitung eines kostengünstigen, portablen Point-of-Care-Diagnostiksystems zur elektrochemischen Detektion pathogener RNA-Viren (zunächst am Beispiel von SARS-CoV-2) angestrebt.
Die Aufgabe der nal von minden GmbH bestand in der Erstellung der Spezifikation und der Ausarbeitung des lab on a chip-Systems in Zusammenarbeit mit den Partnern sowie der Validierung des Systems durch Laborversuche. Es konnte ein lab on a chip-Konzept erstellt werden und die grundsätzliche Funktionalität der technischen und analytischen Komponenten bestätigt werden. Es wurden Studienprotokolle zur analytischen Performance, zur Gebrauchstauglichkeit, In-Use Stabilität und Robustheit des Testprotokolls sowie zur Untersuchung von nativem Probenmaterial angefertigt. Die Untersuchungen wurden vorbereitet, konnten aber nicht final durchgeführt werden, da die Herstellung von robusten Labormustern für den Biosensor-Chip sowie für die Herstellung einer integrierten Auswerteeinheit nicht gelungen ist
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