1,720,965 research outputs found
Spezifische monoklonale Antikörper gegen humanes Cystantin B für die Aufklärung seiner physiologischen und pathophysiologischen Bedeutung
Full text linkCystatin B ist ein endogener Proteinaseinhibitor der Typ 1-Cystatine. CSTB hemmt in vitro verschiedene Cysteinproteinasen der Papainfamilie durch nicht-kovalente, reversible Bindung. Mutationen im CSTB-Gen, die zu einer verringerten CSTB-Expression führen, sind verantwortlich für die progressive myoklonische Epilepsie (EPM1). Weitere Funktionen von CSTB, z.B. in der Tumorbiologie, werden diskutiert, Funktionen die wesentlich für die Ausprägung der Charakteristika maligner Tumoren sind und die sich nicht allein auf seine Rolle als Inhibitor zu beschränken scheinen. Für weiterführende Untersuchungen zur Funktionsaufklärung von CSTB auf Proteinebene wurden im Rahmen der vorliegenden Arbeit 6 hochspezifische monoklonale Antikörper gegen CSTB generiert. Sie wurden nach einer modifizierten Variante der Methode von Köhler und Milstein entwickelt. Im ersten Teil der Arbeit wurden die monoklonalen Antikörper gegen CSTB charakterisiert (Epitope mapping, Bindungskonstanten). Im zweiten Teil wurde mit Hilfe der gereinigten Antikörper ein quantitativer Sandwich-ELISA etabliert, welcher für die Bestimmung von CSTB im Plasma von Karzinom-Patienten genutzt werden soll. Als Nachweis für die Eignung des entwickelten Sandwich-ELISA zur CSTB-Bestimmung im Plasma wurde eine Pilotstudie mit 45 Proben von gesunden Probanden und 32 Proben von HCC-Patienten durchgeführt.von Gerit Thei
Etablierung und Evaluierung der in vivo Isolation von zirkulierenden Tumorzellen für die individualisierte Diagnostik des Prostatakarzinoms
Full text linkDie Therapieoptionen für metastasiertes Prostatakarzinom werden zielgerichteter, erfordern aber wiederholte Tumorbeurteilungen. Die konventionelle Nadelbiopsie ist invasiv und nicht immer möglich. Zirkulierende Tumorzellen (CTCs) könnten als blutbasierte Biomarker eine Alternative bieten. CTCs spiegeln Tumorsubklone wider, jedoch ist ihre geringe Konzentration im Blut eine Herausforderung. Bisherige Isolationstechnologien sind durch das geringe Blutvolumen limitiert. Diese Habilitation untersucht den CellCollector (CC), eine in vivo CTC-Isolationstechnologie. Der CC, ein mit EpCAM-Antikörpern beschichteter Draht, verbleibt 30 min in der Cubitalvene und ermöglicht die Untersuchung von bis zu 600 ml Blut. Ex vivo und in vivo Studien zeigen, dass der CC CTCs mit höherer Sensitivität als das CellSearch-System isoliert, insbesondere bei metastasiertem kastrationsresistentem Prostatakarzinom. Der CC unterstützt die Überwachung und personalisierte Therapie durch sensitive CTC-Isolation.Therapeutic options for metastatic prostate cancer are becoming more targeted but require repeated tumor assessments. Conventional needle biopsy is invasive and not always feasible. Circulating tumor cells (CTCs) could offer an alternative as blood-based biomarkers. CTCs reflect tumor subclones; however, their low concentration in the blood is a challenge. Existing isolation technologies are limited by the small blood volume that can be processed. This habilitation examines the CellCollector (CC), an in vivo CTC isolation technology. The CC, a wire coated with EpCAM antibodies, remains in the cubital vein for 30 minutes and allows for the examination of up to 600 ml of blood. Ex vivo and in vivo studies show that the CC isolates CTCs with higher sensitivity than the CellSearch system, especially in metastatic castration-resistant prostate cancer. The CC supports monitoring and personalized therapy through sensitive CTC isolation
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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