1,721,486 research outputs found
Fattori angiogenetici nelle malattie cardiovascolari: realtà, problemi e futuri sviluppi. Angiogenetic growth factors in cardiovascular medicine: reality, pitfalls and future developments.
No full text
Prognostic factors in acute promyelocytic leukemia: strategies to define high-risk patients
No full textAll trans retinoic acid (ATRA) has revolutionized the therapy of acute promyelocytic leukemia (APL). Treatment of this leukemia with ATRA in combination with chemotherapy has resulted in complete remission rates >90 % and long-term remission rates above 80 %. Furthermore, the combination of ATRA and arsenic trioxide (ATO) was shown to be safe and effective in frontline treatment and, for patients with low and intermediate risk disease, possibly superior to the standard ATRA and anthracycline-based regimen. However, in spite of this tremendous progress, APL still remains associated with a high incidence of early death due to the frequent occurrence of an abrupt bleeding diathesis. This hemorrhagic syndrome more frequently develops in high-risk APL patients, currently defined as those exhibiting >10 × 10(9)/L WBC at presentation. In addition to high WBC count, other molecular and immunophenotypic features have been associated with high-risk APL. Among them, the expression in APL blasts of the stem/progenitor cell antigen CD34, the neural adhesion molecule (CD56), and the T cell antigen CD2 help to identify a subset of patients at higher risk of relapse and often the expression of these markers is associated with high WBC count. At the molecular level, the short PML/RARA isoform and FLT3-internal tandem duplication (ITD) mutations have been associated with increased relapse risk. These observations indicate that extended immunophenotypic and molecular characterization of APL at diagnosis including evaluation of CD2, CD56, and CD34 antigens and of FLT3 mutations may help to better design risk-adapted treatment in this disease
Supramaximal exercise mobilizes haematopoietic progenitors and reticulocytes in athletes
No full textAm J Physiol Regul Integr Comp Physiol. 2005 Nov;289(5):R1496-503. Epub 2005 Jul 14.
Supramaximal exercise mobilizes hematopoietic progenitors and reticulocytes in athletes.
Morici G, Zangla D, Santoro A, Pelosi E, Petrucci E, Gioia M, Bonanno A, Profita M, Bellia V, Testa U, Bonsignore MR.
SourceDepartment of Experimental Medicine, University of Palermo, Italy.
Abstract
Marathon runners show increased circulating CD34+ cell counts and postexercise release of interleukin-6 (IL-6), granulocyte-colony stimulating factor (G-CSF) and flt3-ligand (Bonsignore MR, Morici G, Santoro A, Pegano M, Cascio L, Bonnano A, Abate P, Mirabella F, Profita M, Insalaco G, Gioia M, Vignola AM, Majolino I, Testa U, and Hogg JC. J Appl Physiol 93: 1691-1697, 2002). In the present study we hypothesized that supramaximal ("all-out") exercise may acutely affect circulating progenitors and reticulocytes and investigated possible mechanisms involved. Progenitor release was measured by flow cytometry (n = 20) and clonogenic assays (n = 6) in 20 young competitive rowers (13 M, 7 F, age +/- SD: 17.1 +/- 2.1 yr, peak O2 consumption: 56.5 +/- 11.4 ml.min(-1).kg(-1)) at rest and shortly after 1,000 m "all-out." Release of reticulocytes, cortisol, muscle enzymes, neutrophil elastase, and several cytokines/growth factors was measured. Supramaximal exercise doubled circulating CD34+ cells (rest: 7.6 +/- 3.0, all-out: 16.3 +/- 9.1 cells/mul, P < 0.001), and increased immature reticulocyte fractions; AC133+ cells doubled, suggesting release of angiogenetic precursors. Erythrocyte burst forming units and colony forming units for granulocytes-monocytes and all blood series increased postexercise by 3.4-, 5.5-, and 4.8-fold, respectively (P < 0.01 for all). All-out rowing acutely increased plasma cortisol, neutrophil elastase, flt3-ligand, hepatocyte growth factor, VEGF, and transforming growth factor-beta1, and decreased erythropoietin; K-ligand, stromal-derived factor-1, IL-6, and G-CSF were unchanged. Therefore, all-out exercise is a physiological stimulus for progenitor release in athletes. Release of reticulocytes and proangiogenetic cells and mediators suggests tissue hypoxia as possibly involved in progenitor mobilization.
PMID:16020520[PubMed - indexed for MEDLINE
Primjena Slug testa u formacijama visoke hidrauličke vodljivosti
Full text linkU radu je dan teorijski i praktični prikaz metode slug testa u formacijama visoke hidrauličke vodljivosti. Uvodni dio naglašava važnost vode kao jednog od glavnih resursa kako za sam život ljudi tako i za gospodarstvo koje je u stalnom porastu. Zatim se opisuje voda u tlu koja može zauzimati prostor svih pora u elementu tla (tlo je zasićeno vodom), i djelomičan prostor pora u elementu tla (djelomično zasićeno ili nezasićeno tlo). Objašnjava se pojam vodonosnog sloja i prikazuje se podjela vodonosnih slojeva na otvoreni, poluotvoreni, poluzatvoreni i zatvoreni vodonosni sloj. U poglavlju 3. napravljen je teorijski osvrt o karakterističnim hidrauličkim parametrima vodonosnog sloja (hidraulička vodljivost (K), koeficijent transmisivnosti (T), te koeficijent uskladištenja (S)). Analizira se Darcy-ev zakon i naglašava se razlika između koeficijenta propusnosti (k) i hidrauličke vodljivosti (K). Nakon toga opisuje se pojam slug testa, te su prikazane prednosti slug testa u odnosu na druge terenske metode. U poglavlju 4.3 dan je općeniti pregled postupka analize slug testa, i objašnjeni su koraci od kojih se analiza sastoji. Objašnjenje koraka postupka analize potkrijepljeno je slikama iz MS Excela koje su izrađene na temelju podataka dobivenih na terenu. U nastavku se detaljno opisuje primjer terenske primjene slug testa na lokaciji crpilišta „Varaždin“, gdje je navedena oprema i sam postupak izvođenja slug testa na terenu. Opisana je i izvedba eksploatacijskog zdenca Z-1 i pijezometra PDS-1 u kojem je izvedeno ispitivanje slug testom. Nakon opisivanja terenske primjene slug testa navode se postupci obrade dobivenih podataka na terenu. Obrada podataka izvršena je u programu MS Excel i u programskom paketu AQTESOLV kako bi se mogla vidjeti usporedba podataka tih dvaju softvera. Na kraju je prikazana usporedba rezultata slug testa i probnog crpljenja (probno crpljenje je izvodio Građevinski fakultet, Zagreb), te je utvrđeno kako se odstupanja rezultata nalaze u dozvoljenim granicama od 30-ak %
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Altered growth factor sensitivity in EL2 rat fibroblasts: influence of this biological characteristic on cell growth
No full textExtensive evidence indicate that platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) play a key role in the stimulation of the 3T3 fibroblast replication: in this connection, PDGF and EGF act as a competence and a progression factor, respectively. We have previously demonstrated that EGF alone leads density-arrested EL2 rat fibroblasts to synthesize DNA and proliferate in serum-free cultures. Here, we have analyzed the role of EGF in the control of EL2 cell proliferation. Our data show a dose-related effect of EGF on DNA synthesis and cell growth, with maximal stimulation for both parameters at 20 ng/ml. On the other hand, autocrine production of PDGF or PDGF-like substances by EL2 cells is seemingly excluded by experiments with anti-PDGF serum or medium conditioned by EL2 fibroblasts. EGF binding studies show that EL2 cells possess high affinity EGF receptors, at a density level 3 to 4-fold higher than other fibroblastic lines. In addition, EL2 cells show a normal down-regulation of EGF receptors, following exposure to EGF, but PDGF, fibroblast growth factor (FGF), transforming growth factor beta (TGF beta) and bombesin have not decreased the affinity of EGF receptor for its ligand. Moreover, in EL2 cells, the EGF is able to induce the synthesis of putative intracellular regulatory proteins that govern the PDGF-induced competence in 3T3 cells. Our data indicate that EGF in EL2 cells may act as both a competence and a progression factor, via induction of the mechanisms, regulated in other cell lines by cooperation between different growth factors.(ABSTRACT TRUNCATED AT 250 WORDS
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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