178,191 research outputs found
Mesopore-assisted Profiling of Human Synovial Fluid
No full textMesopore-assisted Profiling of Human Synovial Fluid
Mariaimmacolata Preianòa, Stella Frascàa, Domenico Scopellitia, Antonio Coriglianob, Luca Gallellia, Olimpio Galassob, Giorgio Gasparinib, Rocco Savinoa and Rosa Terraccianoa*
a) Dept. of Health Sciences, University of Catanzaro Magna Græcia, Catanzaro, Italy;
b) Dept. of Medical and Surgical Sciences, University of Catanzaro Magna Græcia, Catanzaro, Italy
Progress in the field of nanostructured materials has provided innovative devices, particularly those based on mesoporous silica (MPS), which have proven to be successful for protein fractionation. Our group has explored a new application of MPS by developing a strategy based on MPS in combination with MALDI-TOF MS for profiling low molecular weight human bodily fluids peptides [1]. Based on a molecular cut-off mechanism, we used MPS as sponges to “capture” peptides present in human bodily fluids. By the mean of controllable pore size and surfaces properties, MPS were used to harvest peptides from human body fluids, excluding large size and high abundant proteins from adsorptive process [2]. We are now revisiting our strategy based on MPS for rapidly profiling synovial fluid (SF) peptidome. SF is an highly viscous fluid with lubricant properties which contains a large number of proteins originating from synovial tissue, cartilage, and serum [3]. SF also contains large sized carbohydrate polymers of hyaluronic acid, which seriously hinder the MALDI-MS detection of peptides in unprocessed samples. In particular, here we describe the protocol which we have developed for SF peptidome profiling by differently functionalized MPS particles for SF peptidome enrichment. The assessment of SF pre-processing with or without hyaluronidase before MPS-MALDI-TOF MS analysis will also be treated with special focus on peptide recovery and analytical reproducibility. Finally, a comparison with different commercially available SPE kits and MSP performances will be shown.
* Corresponding author: Rosa Terracciano, Prof.
University Magna Graecia Catanzaro, Europa Avenue, 88100 Catanzaro, Italy.
Tel.: +39 0961 369 4085;
fax: +39 0961 369 4090.
E-mail address: [email protected] (R. Terracciano)
References:
[1] Savino R, Terracciano R. Mesopore-assisted profiling strategies in clinical proteomics for drug/target discovery. Drug Discov Today. 2012;17(3-4):143-52.
[2] Terracciano R, Pasqua L, Casadonte F, Frascà S, Preianò M, Falcone D, Savino R. Derivatized mesoporous silica beads for MALDI-TOF MS profiling of human plasma and urine. Bioconjug Chem. 2009;20(5):913-23.
[3] Hu S, Loo JA, Wong DT. Human body fluid proteome analysis. Proteomics. 2006;6(23):6326-53
Supplementary Material For "Pattern-Reversal Visual Evoked Potential on Smart Glasses"
No full textThis dataset contains post-processed Pattern Reversal Visual Evoked Potential (PR-VEP) signals from 20 subjects, 12 males and 8 females between 24-28 years old. These signals are acquired with two different kinds of instruments: a new prototype based on Smart Glasses and a commercial device for PR-VEP.For a detailed description of this dataset see accompanying publication "Pattern-Reversal Visual Evoked Potential on Smart Glasses" by Terracciano R., et al. IEEE Journal of Biomedical and Health Informatics 2019, 10.1109/JBHI.2019.2899774
Update on sutureless thyroidectomy
No full textSummary: Modified transverse coloplasty pouch: new reconstruction techniques after total mesorectal excision, our experience. r. ruGGIero, G. DocImo, a. BoSco, m. lanzavolPe, G. terraccIano, a. GuBItoSI, l. DocImo Introduction. The Harmonic scalpel (“Focus“ and the new version “Focus+”) is one of the first devices for surgical simultaneous cutting and tissue coagulation which allows to obtain dissection and hemostasis by direct application of ultrasound and allows minimally invasive surgical procedures with minimal lateral thermal spread and, thus, minimal adjacent tissue destruction. The aim of the study is to complete the previous study that we made in 2014, based on the TT performed between January 2008 and December 2013, with new data about TT performed in our Surgical Division between January
2014 and December2016 and compare the outcome using the Ultrasonic scalpel versus the device Ligasure in term of safety, operative time, overall drainage volume, complications, hospital stay. materials and methods. 250 patients were randomized into two groups: Group A where Ultracision were used and Group B where the Ligasure device was used. Results. The results of the group A and of the Group B 2014-2016 have been compared with the results of the previous study and we found that they are similar, but asymptomatic hypocalcaemia increased in the patients of the new study. conclusions. We found that the use of Ultrasonic scalpel and Ligasure is effective both in the hemostasis of all vessels and in dissection of tissues and confirm the results of our first study without significant difference in the rate of post-operative morbidity with these two different energy based devices used
HMGA1 and HMGA2 protein expression correlates with advanced tumour grade and lymph node metastasis in pancreatic adenocarcinoma.
No full textPiscuoglio S, Zlobec I, Pallante P, Sepe R, Esposito F, Zimmermann A, Diamantis I, Terracciano L, Fusco A & Karamitopoulou E (2012) Histopathology 60, 397-404 HMGA1 and HMGA2 protein expression correlates with advanced tumour grade and lymph node metastasis in pancreatic adenocarcinoma Aims: Pancreatic ductal adenocarcinoma follows a multistep model of progression through precursor lesions called pancreatic intraepithelial neoplasia (PanIN). The high mobility group A1 (HMGA1) and high mobility group A2 (HMGA2) proteins are architectural transcription factors that have been implicated in the pathogenesis and progression of malignant tumours, including pancreatic cancer. The aim of this study was to explore the role of HMGA1 and HMGA2 in pancreatic carcinogenesis. Methods and results: HMGA1 and HMGA2 expression was examined in 210 ductal pancreatic adenocarcinomas from resection specimens, combined on a tissue microarray also including 40 examples of PanIN and 40 normal controls. The results were correlated with the clinicopathological parameters of the tumours and the outcome of the patients. The percentage of tumour cells showing HMGA1 and HMGA2 nuclear immunoreactivity correlated positively with increasing malignancy grade and lymph node metastasis. Moreover, HMGA1 and HMGA2 expression was significantly higher in invasive carcinomas than in PanINs. No, or very low, expression was found in normal pancreatic tissue. Conclusions: Our results suggest that HMGA1 and HMGA2 are implicated in pancreatic carcinogenesis and may play a role in tumour progression towards a more malignant phenotype
3D SURVEY AND MATERIAL CHARACTERIZATION OF THE ARCHAEOLOGICAL COMPLEX OF THE STADIUM OF ANTONINO PIO IN POZZUOLI
No full textThe presented work is an expansion of practice exercise carried out for a diploma thesis of the School of Specialization of Architectural Heritage and Landscape of the University of Naples Federico II, in agreement with the Archaeological Park of Phlegraean Fields, and illustrates the results of the 3D survey and material characterization campaign of the archaeological complex of the stadium of Antonino Pio in Pozzuoli. It is located on the western border of the Roman city of Puteoli and was built by Emperor Antonino Pio to host Eusebia, the quinquennial ludi instituted in Hadrian’s memory. After the 6th century, the area was gradually abandoned until it was buried by volcanic ash in 1538. Since the start of the 21st century, archaeological excavations were performed, together with the first series of safety works on the structures and excavation fronts.
This research work fits in the phase of analysis and material characterization, which is preliminary to the elaboration of a restoration project. It consisted of the 3D survey of the structure with laser scanning and photo modeling techniques through aerial drone shots and was aimed at the individuation of the construction typologies and ongoing decay phenomena.
The continuous reference between the results of the survey and those from the comparative analysis of historical and archival documentation has allowed the reconstruction of its architectural history and the sequence of its recent restorations, hence defining an integrated and implementable picture, which is necessary for the realization of suitable strategies of conservation and fruition
- …
