170,483 research outputs found
Field study results on the probability and risk of a horizontal gene transfer from transgenic herbicide-resistant oilseed rape pollen to gut bacteria of bees
No full textBees are specifically subjected to intimate contacts with transgenic plants due to their feeding activities on pollen. In this study, the probability and ecological risk of a gene transfer from pollen to gut bacteria of bees was investigated with larvae of Apis mellifera (honeybee), Bombus terrestris (bumblebee), and Osmia bicornis (red mason bee), all collected at a flowering transgenic oilseed rape field. The plants were genetically engineered with the pat-gene, conferring resistance against glufosinate (syn. phosphinothricin), a glutamine-synthetase inhibitor in plants and microorganisms. Ninety-six bacterial strains were isolated and characterized by 16S rRNA gene sequencing, revealing that Firmicutes represented 58% of the isolates, Actinobacteria 31%, and Proteobacteria 11%, respectively. Of all isolates, 40% were resistant to 1 mM glufosinate, and 11% even to 10 mM. Resistant phenotypes were found in all phylogenetic groups. None of the resistant phenotypes carried the recombinant pat-gene in its genome. The threshold of detecting gene transfer in this field study was relatively insensitive due to the high background of natural glufosinate resistance. However, the broad occurrence of glufosinate-resistant bacteria from different phylogenetic groups suggests that rare events of horizontal gene transfer will not add significantly to natural bacterial glufosinate resistance
Fortlaufende Beobachtung elementarer Vokabularentwicklung unterstützt sprechender Kinder: Ein Beobachtungsbogen zur Diagnostik und Förderung
No full textTebbe M, Zurbriggen C. Fortlaufende Beobachtung elementarer Vokabularentwicklung unterstützt sprechender Kinder: Ein Beobachtungsbogen zur Diagnostik und Förderung. Zeitschrift für Heilpädagogik. 2022;73(5):200-215
Comparison of compost and vermicompost bacterial diversity by SSCP analysis and gene probe hybridization.
No full textBacterial diversity of compost and vermicompost by means of a cultivation-independent community analysis and gene probe hybridization
No full textBacterial Diversity of Compost and Vermicompost: A Cultivation-Independent Community Analysis.
No full textDiversità batterica di compost e vermicompost: un’analisi di comunità coltivazione-dipendente.
No full textOlefin Metathesis in Cyclic Ether Formation. Direct Conversion of Olefinic Esters to Cyclic Enol Ethers with Tebbe-Type Reagents
No full textOlefin Metathesis in Cyclic Ether Formation.
Direct Conversion of Olefinic Esters to Cyclic Enol
Ethers with Tebbe-Type Reagent
Olefin Metathesis in Cyclic Ether Formation. Direct Conversion of Olefinic Esters to Cyclic Enol Ethers with Tebbe-Type Reagents
No full textOlefin Metathesis in Cyclic Ether Formation.
Direct Conversion of Olefinic Esters to Cyclic Enol
Ethers with Tebbe-Type Reagent
Allergens and molecular diagnostics of shellfish allergy
No full textIn recent years, there has been a steady growth in the production and consumption of shellfish due to its important role in human nutrition and health. This increased consumption has led to an increase in adverse health problems among consumers including immunoglobulin E (IgE)-mediated allergic reactions. Approximately 2 % of the world population is affected by shellfish allergy, which includes the crustacean and mollusk groups. The allergenic proteins present in the shellfish group have variable primary structures and often present a challenge in allergen detection and diagnosis. The distinction of crustacean from mollusk is important from clinical point of view, as molecular cross-reactivity, particularly between crustaceans, seems to be determined by the close relationship to insects and mites. Currently, at least seven different shellfish allergens have been identified, mostly from crustaceans; however, only three recombinant allergens are available for IgE-based routine diagnostic, including tropomyosin, arginine kinase, and sarcoplasmic Ca++-binding protein. Other allergens include myosin light chain, troponin C, triose-phosphate isomerase, and actin.
This chapter describes current information on shellfish allergy, the allergenic proteins involved, and diagnostic approaches
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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