15 research outputs found
Analysis of the effect of endophytic bacteria on the carbonylation of tobacco (nicotiana tabacum) cell proteins
No full textNors yra daug informacijos apie endofitinius mikroorganizmus, žinios apie augalų ir endofitų molekulinės sąveikos mechanizmus ir procesus vykstančius pradiniame augalo audinių kolonizacijos etape yra nepakankamai išsamios. Todėl tyrimo tikslas in vitro modelinėje sistemoje ištirti ankstyvosios kolonizacijos etape vykstančius procesus ir endofitinių bakterijų poveikį augalui.
Darbe aprašomi tyrimai buvo atliekami naudojant paprastojo tabako Nicotiana tabacum L. ląstelių kultūrą ir šešis skirtingus endofitinių bakterijų kamienus. Tyrimo metu buvo ištirtas endofitinių bakterijų poveikis paprastojo tabako Nicotiana tabacum L. ląstelių kultūros gebėjimui kaupti aktyviuosius deguonies junginius. Šis tyrimas buvo atliktas naudojant dichlorofloresceino diacetato fluorescencijos metodą, o viena . Gauti rezultatai rodo, jog viena iš tirtų bakterijų statistiškai mažino ADJ kaupimą. Dėl teigiamo poveikio aktyvių deguonies junginių kaupimui, tolimesniems tyrimams buvo pasirinkta Bacillus Simplex NT21. bakterija.
Sekančio tyrimo metu buvo atliktas skirtingų fluorescuojančių dažų stabilumo vertinimas skirtingose temperatūrose ir pasirinkti stabiliausi ir labiausiai tinkami dažai. Atlikus dažų stabilumo vertinimą, buvo pasirinkta dvikryptės elektroforezės metodui naudoti CF647DI dažus.
Pasirinkus tinkamiausią endofitinės bakterijos kamieną ir fluorescuojančių dažų tipą, buvo atliktas šių bakterijų inokuliacijos poveikio tabako ląstelių baltymų kaupimui ir oksidacinei modifikacijai tyrimas, naudojant dvikryptės elektroforezės metodą. Gauti rezultatai rodo, jog ištirtas endofitinės bakterijos kamieno aktyvinamas ADJ kaupimas tabako ląstelėse sukelia streso atsako, branduolio rūgščių sintezės ir metabolizmo procesuose dalyvaujančių baltymų karbonilinimo pokyčius.The studies described in this paper were carried out using a cell culture of Nicotiana tabacum L. and six different strains of endophytic bacteria. Knowledge of the molecular mechanisms of plant-endophyte interactions and the processes involved in the initial stages of colonisation of plant tissues is incomplete and lacking. Therefore, the aim of this study was to investigate in an in vitro model system the processes involved in the early colonisation phase and the effects of endophytic bacteria on the plant.
The study investigated the effect of endophytic bacteria on the cell culture of N. tabacum L. cells abilities to accumulate reactive oxygen species. This study was carried out using the dichloroflorescein diacetate fluorescence method. The results show that one of the six bacteria tested showed a statistically significant increase in the accumulation of ROS and one other strain showed an inhibition.
In the following experiment, the stability of different fluorescent dyes at different temperatures was evaluated and the most stable and suitable dyes were selected. After the stability assessment, the CF647DI dye was chosen for the bidirectional electrophoresis method.
After selecting the most suitable endophytic bacterial strain and type of fluorescent dye, the effect of inoculation of these bacteria on the accumulation and oxidative modification of tobacco cell proteins was investigated by means of bidirectional electrophoresis. The results show that this endophytic bacterial strain activated by the accumulation of reactive oxygen species in tobacco cells induces changes in the stress response, the synthesis of nuclear acids, and in the carbonylation of proteins involved in metabolic processes.Gamtos mokslų fakultetas / Faculty of Natural SciencesVytauto Didžiojo universitetas / Vytautas Magnus UniversityBiologijos katedra / Department of Biolog
X - ray sensitive organic materials with carbazol and difenilamine functional groups investigation of electrical properties.
No full textAuthor: Edgaras Balandis Title of bachelor project: X - ray sensitive organic materials with carbazol and difenilamine functional groups investigation of electrical properties. Organic layers of, K – 31, K – 235, K – 179, K – 144, K – 221 were formed on ITO and aluminium by two – way heating element. Two – way heating method was selected in order to avoid cooling of material from one side of the sample. All samples were made using 5 grams of each material. Melting points of materials observed were between 240 ºC and 270 ºC. Thickness of sample layers were between 8,8 µm and 54,4 µm. For concurrent measuring tasks similar thickness samples have been used. The volt-ampere characteristics of all materials illuminated by X - rays were measured. Received photocurrent values were compared with each other, calculating the ratio of dark current and photocurrent. Measured induced currents dependence of X – ray flux in rage from 0.1 mA to 1 mA. The results are compared with a linearly distribution. Calculated intrinsic electrical conductivity from 2.52 S/m x 10–13 to 7.04 S/m x 10–13. Measured temporal dependence of X – ray induced currents with all materials. Temporal dependence were measured in intervals of 100 s. Received values mapped in chart and calculated rise time from 1.35 s to 6.14 s and fall time from 0.33 s to 1.07 s. All organic materials used in this work were sensitive to X – ray. Material structural differences in the field of aniline affect samples photocurrent result
Redox enzymes involved in arabidopsis thaliana (L.) heynh seedling response to cold plasma treatment of seeds
No full texthttp://www.openreadings.eu/wp-content/uploads/2021/03/Abstract_book_2021S.pdfSuccessful seed germination and seedling establishment are critical steps in agricultural production and in the maintenance of natural ecosystems. Seed treatment with cold plasma (CP), is an emerging eco-agricultural technology that has potential application for enhancement of plant growth and adaptation. In addition to sterilization effect, treatment of seeds with CP has been shown to have effect on a broad spectrum of plant development and physiological processes. Although the body of information on the biological effects of the CP is growing, the complex biological mechanisms involved in the plant response remains vague. The objective of this study was to assess role of reactive oxygen species (ROS) producing and redox balance regulating enzymes in response of the model plant Arabidopsis thaliana (L.) Heynh. to the seed treatment with atmospheric pressure dielectric barrier discharge plasma source. Seeds of A. thaliana Columbia ecotype wild-type (Col-0) and gene knockout mutant lines of ROS producing enzymes NADPH oxidase AtRbohB and AtRbohF and enzymes involved in maintenance of the cellular redox balance, AtAPX1 and AtCAT2, were used in the study. Seeds were treated for 1.5 or 3.0 min at 3 mm distance from the CP source at 60-80% air humidity and stored for 7 days at 25 oC after the treatment. The significant changes in root length was observed for the 2 day old seedlings of Col-0, AtRbohB, AtRbohF and AtCAT2 germinated from the seeds treated for 3 min. AtRbohF showed an enhanced (~6 % compared to control) root growth response similar to that of the wild-type genotype, meanwhile the length of roots was reduced for the AtRbohB and AtCAT2 mutants (20 % and 10 %, respectively). In contrast to the effect observed for the roots, 3 min CP treatment had stimulating effect on hypocotyl development of the AtAPX1 mutant (~7 %). Meanwhile hypocotyls of the AtRbohF and AtCAT2 were shorter compared to control[...]Gamtos mokslų fakultetas / Faculty of Natural SciencesVytauto Didžiojo universitetas / Vytautas Magnus Universit
Acting in socialist realist theatre: theoretical – Ideological contexts and the analysis of Lithuanian theatre
No full textDarbe nagrinėjami socialistinio realizmo doktrinos principai vaidyboje ir jos pedagogikoje ir siekiama identifikuoti šiuolaikinės vaidybos problemų sąsajas su sovietinės okupacijos metu vykusiais pokyčiais ir procesais Lietuvos teatre. Darbe bandoma rekonstruoti socialistinio realizmo vaidybos teorija, kuri buvo sukurta remiantis Konstantino Stanislavskio vaidybos sistemos pagrindu. Sovietmečio vaidybos estetika ir pedagogika darbe analizuojama per politinės doktrinos tvirtinimo ir siekio skleisti ideologiją prizmę suvokiant socrealizmą ne tik kaip represinę ar kontrolės funkciją vykdančią jėgą, bet ir kaip galios principus diktuojančią ir naujos raiškos formų atsiradimo terpę.
Šio darbo objektas yra teoriniai tekstai, pedagogika ir kūrybinės praktikos Lietuvos teatre.
Šio darbo tikslas yra išanalizuoti socialistinio realizmo vaidybos teorinę ir ideologinę sampratas bei jų reikšmes ir atspindžius vaidybos pedagoginėse praktikose ir teatro spektaklių vaidmenyse.
Kad šis tikslas būtų pasiektas, keliami šie uždaviniai:
1. Tirti šaltinius, šaltinių grupes ir juos kritiškai vertinti;
2. Tirti šiuolaikinių autorių studijų rezultatus, nagrinėjančius socialistinį realizmą;
3. Tirti ir aprašyti socialistinio realizmo poveikį teatrinėms institucijoms;
4. Tirti ir aprašyti socialistinio realizmo poveikį kitoms meno sritims;
5. Tirti ir aprašyti socialistinio realizmo vaidybos teorijos gaires;
6. Tirti ir aprašyti socialistinio realizmo vaidybos pedagogikos metodologijos gaires;
7. Išanalizuoti socrealistinės vaidybos atvejus praktikoje.
Pirmoje darbo dalyje apžvelgiama ir analizuojama socialistinio realizmo samprata, teorinės prielaidos ir raida. Antroje - gilinamasi į socialistinį realizmą Lietuvos dramaturgijoje ir teatre. Trečioje darbo dalyje tiriama socialistinio realizmo vaidybos teorija ir pedagogika. Ketvirtoje- bandoma rekonstruoti ir atskleisti socrealistinės vaidybos bruožus nagrinėjant konkrečius praktikos atvejus Lietuvos teatre.The thesis analyses social realism doctrine principles in acting and acting pedagogy and seeks to identify modern acting problem interfaces with the soviet ocupation timeline changes and processes in Lithuanian theatre. The study tries to reconstruct the acting theory of socialist realism, which was created based on the basis of Konstantin Stanislavsky's acting system. The soviet ocupation timeline acting esthetic and pedagogy are analysed in the study through the prism of the political doctrine fastening and the goal to spread ideology, understanding social realism not only as a represive or control type function executing power, but also as a power principle dictating and the emergence of a new form of expression medium .
The object of this thesis is theoretical texts, pedagogy and artistic practices in Lithuanian theatre.
The goal of this thesis is to analyse the theoretical and ideological acting concepts in social realism, furthermore their meanings and reflections in the pedagogy practices of acting and in the roles of theatre plays.
For this goal to be achieved, these objectives are set:
1. Study sources, source groups and critically rate them;
2. Research modern authors' study results of their analysis of social realism ;
3. Study and describe the effect of social realism in theatrical institutions;
4. Study and describe the effect of social realism to other fields of art;
5. Study and describe the guidelines of the social realism acting theory;
6. Study and describe the guidelines of the social realism acting pedagogy methodology;
7. Analyse social realism acting cases in practice.
It the first part of tthis thesis author reviews and analyses the concept, theoretical assumptions and development of social realism. The second part focuses on social realism in Lithuanian dramaturgy and theatre. The third part studies the acting theory and pedagogy in social realism. The fourth part tries to reconstruct and reveal features of social realism by studying specific cases in practice in Lithuanian theatre.Vytauto Didžiojo universitetas / Vytautas Magnus UniversityMenų fakultetas / Faculty of ArtsTeatrologijos katedra / Department of Theater Studie
Evolution of the legal concept of working time.
No full textEvolution of the Legal Concept of Working Time Working time is a crucial aspect of the employment relationship, under which the parties to the employment relationship create exclusive rights and obligations to each other. Accordingly, the regulation of working time ensures a legal balance between the parties to the employment relationship and guidelines on the basis of which the employer and the employee can seek mutual benefits and, accordingly, perform their duties correctly. In order to understand the origins of the current regulation of working time, the work retrospectively looks at the former regulation of working time, as well as evaluates the current concept of working time. The written work examines the 19th century legislation governing working time. By evaluating and comparing them, the aim is to determine the legal status, working time norms and concept of working time in the English Empire, the German / Prussian Empire, and the French Empire. Assessing the 19th century legislation, the author examines the 20th century. norms of working time law arising in the context of international law. The written work examines the globally ratified International Labor Organization conventions, as well as regional European legislation, evaluates the concepts of working time presented in them, and monitors their compliance with the 19th century legislation. The work also pays a lot of attention to the concept of working time presented in Lithuanian national law and the evolution of working time in different legal acts of labor law. After analyzing the laws regulating working time of the Republic of Lithuania and currently regulating it, the author compares the compliance of national law with international legal acts in terms of working time
Kriminālās izlūkošanas pasākumu ierobežojumi likuma izpildes direktīvā un Lietuvas kriminālās izlūkošanas likumā
No full textIncreasing use of technologies in the last decades has created an unprecedented opportunity to systematically collect and use a wide variety of data (including personal data) for different purposes. Information and data collected and processed with the help of new technologies is used not only for the purposes of natural and legal persons but also for various other purposes. Intelligence services that ensure prevention of crime must perform their functions to ensure safety of public. When doing so, they use various means and methods of information collection, which help them to reach their goals. However, the means applied undermine and intensively restrict a person’s right to private life. Given that two legal interests compete during the application of criminal intelligence measures, i.e. the individual’s right to privacy and ensuring of public security, the Author seeks to analyse their points of contact – restrictions of application of criminal intelligence measures, which in theory are designed to ensure the person’s right to private life. In this article, the Author analyses the restrictions on the application of criminal intelligence measures, which are present in international, Lithuanian legislation and compares them with relevant requirements set forth in the practice of European Union Court of Justice.Pieaugošā tehnoloģiju izmantošana pēdējās desmitgadēs ir radījusi nepieredzētu iespēju sistemātiski ievākt un izmantot ļoti dažādus datus (ieskaitot personas datus) dažādiem mērķiem. Informācija un dati, kas ievākti un apstrādāti ar jauno tehnoloģiju palīdzību, tiek izmantoti ne tikai fizisko un juridisko personu vajadzībām, bet arī dažādiem citiem mērķiem. Izlūkošanas dienestiem, kas nodrošina noziedzības novēršanu, jāveic savas funkcijas, lai nodrošinātu sabiedrības drošību. To darot, viņi izmanto dažādus informācijas vākšanas līdzekļus un metodes, kas viņiem palīdz sasniegt savus mērķus. Tomēr izmantotie līdzekļi nereti grauj un intensīvi ierobežo personu tiesības uz privāto dzīvi. Tā kā kriminālizlūkošanas pasākumu piemērošanā sacenšas divas likumīgas intereses – personas tiesības uz privātumu un sabiedrības drošības nodrošināšana –, autore cenšas analizēt to saskares punktu – kriminālizlūkošanas pasākumu piemērošanas – ierobežojumus, kas teorētiski ir izstrādāti, lai nodrošinātu personas tiesības uz privāto dzīvi. Šajā rakstā autore ir izvēlējusies analizēt kriminālizlūkošanas pasākumu piemērošanas ierobežojumus: (1) obligāta iepriekšēja kontrole (sankcija) noteiktam kriminālizlūkošanas pasākumam, ko veic tiesa vai neatkarīga administratīva vienība; (2) kriminālizlūkošanas pasākumu ilguma ierobežošana; (3) kriminālizlūkošanas pasākumu samērīgums. Rakstā secināts, ka, kaut arī šie kriminālās izlūkošanas piemērošanas ierobežojumi likuma izpildes direktīvā parasti nepastāv, tie ir ietverti Lietuvas Republikas likumā par kriminālo izlūkošanu. Tomēr ar tiem var viegli manipulēt un tie nenodrošina tiesības uz privātās dzīves efektīvu aizsardzību
Analysis of usage of genetic and tabu search algorithms in shop scheduling.
No full textA wide area of scheduling problem is industrial so called shop scheduling. There are three classes of shop scheduling: Job Shop, Open Shop and Flow Shop. General problem specification could be specified as follows: there is set of jobs and set of machines, which should interact with each other in some specific way. Typically these problems are hard to solve in traditional (exact) methods. Metaheuristics algorithms mostly produce only nearby-optima, but in proper time. We implemented several metaheuristics: genetic algorithms (separated by values of their parameters) and several Tabu search algorithms (separated by neighborhood of solution). Some strategies of genetic algorithms are suggested as conclusion of genetic algorithm parameter research. Eight algorithms are examined for random shop scheduling problems in terms of initial solutions and minimum, gained solutions and minimum, processing time and difference between initial and gained solutions. In the end, author concludes, that one kind of genetic parameters (crossover and mutation rates) are especially demanding in sense of algorithm convergence, diversification and intensification aspects, other (number of iterations and population size) should depend on resources, third (elitism) is good “buffers”. Finally, while with its simplest form, Tabu search seems to be less competitive in algorithm effectiveness research, its dynamic modification outperforms all proposed genetic algorithms, but both – tabu search with enlarged diversification and dynamic parameter strategy of genetic algorithm – performs quite well and requires more solid future studies to compete with other advanced metaheuristics
Destructive adaptation: censorship and the ways to resist it in Lithuanian theatre of the Soviet period
No full textStraipsnio tikslas – apžvelgti šiuo metu Lietuvos kultūrinėje spaudoje vyraujančius sovietinės cenzūros ir pasipriešinimo jai vertinimus ir, remiantis šios epochos lietuvišku teatru, pateikti alternatyvų požiūrį. Straipsnyje analizuojamas vertinamasis požiūris, siūlantis nuosaikų ir radikalų cenzūros veikimo, meninių Ezopo kalbos bei potekstės technikų vertinimą, apžvelgiami cenzūros veikimo vidiniai prieštaravimai (vidinė transgresija) ir suvokėjo fantazijos vaidmuo atskleidžiant politines spektaklio potekstes. Straipsnyje pateikiamas postkolonializmo teorijos siūlomas požiūris, jo reikšmė ir galimas pritaikymas aiškinantis meno kūrinio funkcionavimą sovietinės kultūros erdvėje. Reikšminiai žodžiai: Sovietmečio Lietuvos teatras; Cenzūra; Ezopo kalba; Potekstė; Postkolonializmas; Soviet Lithuanian theatre; Censorship; Aesopian language; Subtext; Post-colonialismThe aim of the study was to survey the attitudes towards the Soviet censorship of artistic texts and possibilities to evade it, prevailing today in the Lithuanian cultural discourse, and to introduce a possible alternative point of view referring to the context of Lithuanian theatre history. The author comments on the normative point of view, with two opposite ways of assessing the outcomes of censorship and the techniques of subtext, analyses the inner ambiguities of the functioning of censorship (inherent transgressions) and the role of spectator’s fantasy in the political effects and functioning of the subtext of theatre performances. Also, the attitude proposed by the post-colonial theory and the possibilities to apply it in the research of the functioning of artistic production in Soviet culture are introduced
Legal Issues of Personal Data Transfer in Cyberspace between the European Union and the United States of America
No full textThis study is intended to analyze the problems of personal data transfer in cyber
space, in the interaction of the European Union and the American legal systems - the
conditions of possible legal grounds for the transfer of personal data from the European
Union to the United States of America, taking into account the protection of national security
interests and the practice formed by the Court of Justice of the European Union in
this aspect. In carrying out this research, in the interpretations of the Court of Justice of
the European Union in the Schrems II case, the author identified a key problematic aspect of the right to privacy and protection of personal data related to the implementation of the
third country’s national security interest. According to the interpretations of the Court of
Justice of the European Union, third country’s interest in having access to personal data
obtained from the entities of the European Union, in defense of its national security, is
evaluated according to much stricter criteria than those of the European Union member
states themselves and, in the author’s opinion, is essentially negated. Therefore, the study
analyzes the concept of national security and, taking into account, first of all, the rulings
of the Court of Justice of the European Union in cases Schrems and Schrems II and the
principle of proportionality, as the main legal test for assessing the legality of restrictions
on a person’s right to privacy and protection of personal data, tries to determine, whether
the transfer of personal data between the European Union and the United States can be
considered legal or not. Finally, the study entails proposals for possible changes to legal
regulation in the United States of America to ensure the legality of personal data transfer
between the European Union and the United States of America
