7,886 research outputs found
TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation
Full text linkThe interaction between TACC3 (transforming acidic coiled coil protein 3) and the microtubule polymerase ch-TOG (colonic, hepatic tumor overexpressed gene) is evolutionarily conserved. Loading of TACC3–ch-TOG onto spindle microtubules requires the phosphorylation of TACC3 by Aurora-A kinase and the subsequent interaction of TACC3 with clathrin to form a microtubule binding surface. Whether there is a pool of TACC3–ch-TOG that is independent of clathrin in human cells, and what is the function of this pool, are open questions. Here, we report that TACC3 is recruited to the plus-ends of microtubules by its association with ch-TOG and that this pool is independent of phosphorylation and binding to clathrin. The plus-end binding of TACC3–ch-TOG persists in interphase and we propose that one cellular function of TACC3–ch-TOG is to modulate cell migration. We also describe the distinct subcellular pools of TACC3, ch-TOG and clathrin. TACC3 is often described as a centrosomal protein, but we show that there is no significant population of TACC3 at centrosomes. The delineation of distinct protein pools reveals a simplified view of how these proteins are organized and controlled by post-translational modification
Activation of multifarious transcription of an adhesion protein gene ap65-1 by a novel Myb2 protein in the protozoan parasite Trichomonas vaginalis
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Application of genetic algorithms to parameter estimation of bioprocesses
No full textThe paper explains the application of a genetic algorithm (GA) to the problem of estimating parameters for a kinetic model of a biologically reacting system; It is demonstrated that the GA is a powerful tool for quantifying the kinetic parameters Using kinetic data. As the operation of the GA does not depend on the form of the model equation, it can be applied to the wide spectrum of kinetic modelling problems without any complex formulation procedure
Intervensi Latihan Tai-Chi pada Pasien Kanker yang Mengalami Fatigue
Full text linkThis study aims to analyze the tai-chi exercise intervention as an alternative to reduce fatigue in cancer patients. The method used is a systematic review using the ClinicalKey Nursing, SAGE Journal, ScienceDirect, Scopus, and SpringerLink databases. The results showed that of the 1340 articles found in the initial search, 17 were selected based on their title and abstract. Reports were read, and eight papers were selected according to predetermined criteria. The results showed a positive effect of tai-chi training in reducing fatigue in cancer patients. In conclusion, tai-chi exercise intervention can help relieve symptoms and reduce cancer-related fatigue in cancer patients.
Keywords: Fatigue, Cancer Patients, Tai-ch
Predispersed solvent extraction of negatively complexed copper from water using colloidal liquid aphron containing a quaternary ammonium salt
No full textNegatively complexed copper ion by complexing agent Re EDTA (Ethylenediaminetetraacteic acid) was removed by predispered solvent extraction (PDSE) using colloidal liquid aphrons (CLAs) made out of Trioctylmetylammonium chloride (Aliquat 336) diluted with nonpolar kerosene. PDSE was found to have higher mass transfer rate than conventional solvent extraction under experimental conditions without mechanical mixing. The effect of type of water-soluble surfactants, phase volume ratio (PVR), concentration of anionic Sodium Dodecyl Benzene Sulfonate (SDBS) on PDSE was investigated. In addition, the effect of anionic SDBS on back extraction in PDSE was also studied. Under experimental conditions with enough mechanical mixing, the amount of copper transferred to Aliquat 336 core from the pregnant phase was compared in both PDSE by using anionic SDBS and conventional solvent extraction. It is concluded that PDSE using Aliquat 336 CLA can be used for treatment of negatively complexed copper without the influence of surfactant. To optimize CLAs-based process, stability of CLAs containing a quaternary ammonium salt Aliquat 336 diluted with kerosene in the continuous phase was investigated by measuring the volume released to surface. To destabilize CLAs, H+, OH- were added. Stability of CLAs was estimated by comparing the half-life obtained. Break-up of destabilization follows pseudo-first-order reaction kinetics at low ionic strength. But, pseudo-first-order model cannot be applied to a region of high ionic strength
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