1,730,200 research outputs found
Jung Tae Kim
No full text학위논문(박사)--아주대학교 의학전문대학원 :의학과,2020. 2I. Introduction 1
A. Introduction 1
1. The relation between Glucose, Lipid metabolism and Cancer cell 1
2. The role of miRNA in glucose and lipid metabolism 1
3. The role of miRNA in Cancer Cell 2
4. miRNA-181a-5p and Non Small Cell Lung Cancer 2
5. Hypothesis 3
II. Subjects 4
A. Materials and Methods 4
1. Cell culture and growth conditions 4
2. Glucose-uptake assay 4
3. Lactate dehydrogenase assay 4
4. Mitochondrial ATP synthase Inhibition assay 4
5. Measurement of extracellular acidification rate (ECAR) after treatment of miRNA-181a-5p 5
6. Lipid Staining 5
7. Invasion and migration assays 5
8. TaqMan miRNA expression assay 6
9. miRNA mimic and siRNA transfection 6
10. qRT-PCR 6
11. Western blot 6
12. Approach to search the potential target genes of miRNA-181a-5p 7
13. Luciferase reporter assays 7
14. Statistical analyses 7
B. Results 8
1. Function of miRNA-181a-5p in aerobic glycolysis 8
2. miRNA-181a-5p reduces total lipid in lung cancer cell 9
3. miRNA-181a-5p inhibits lung cancer cell migration and invasion in vitro 9
4. SIRT1 and ACSL4 transcriptions are repressed by binding of miRNA-181a-5p to the 10
5. Quantitative analysis of miRNA-181a-5p, SIRT1, and ACSL 4 expression in lung cancer cell lines 10
6. miRNA-181a-5p regulates SIRT1 and ACSL4 expression at mRNA and protein levels 11
C. Discussion 11
III. Conclusion 14DoctoralmicroRNAs play an important role in cancer metabolisms. miRNA-181a-5p has decreased expression, particularly in non small cell lung cancer tissues and mesenchymal like lung cancer cells. Epithelial-mesenchymal transition mechanisms and cancer metabolism are regulated by the same signaling pathway. Functional analysis was performed to identify the functional role of miRNA-181a-5p in cancer metabolism and to find the target gene. To define the role of miRNA-181a-5p in cancer metabolism, we performed LDH assay, glucose uptake assay, Oil Red O staining, and mitochondrial ATP synthase inhibitor assay. The target gene of miRNA-181a-5p was determined by luciferase reporter assay, qRT-PCR, and western blot analyses. The Functional effect of miRNA-181a-5p on cancer metastasis was measured by migration and invasion assays. Experimental results showed that overexpression of miRNA-181a-5p reduced aerobic glycolysis and adipocytes and decreased cancer cell invasion and migration. Expression of SIRT1 and ACSL4 was reduced by the binding of miRNA-181a-5p to the 3’-UTR. These results suggest that miRNA-181a-5p might participate in the reprogramming of cancer metabolism
Yun Tae Kim
No full text학위논문(석사)----아주대학교 일반대학원 :의학과,2007. 8ABSTRACT i
TABLE OF CONTENTS iii
LIST OF FIGURES v
LIST OF TABLES vi
Ⅰ. INTRODUCTION 1
Ⅱ. MATERIALS AND METHODS 3
A. MATERIALS 3
1. Patients 3
2. Immunohistochemistry 3
3. Cell and Cell culture 4
B. METHODS 4
1. Reverse Transcriptase-Polymerase Chain Reaction 4
2. Western blotting 4
3. Effect of EGF on Proliferation 5
4. Effect of EGF on colony dispersion 5
5. Effect of EGF on wound healing 5
6. Effect of EGF on Invasion 6
7. RT-PCR of MMP-2 and MMP-9 6
8. Zymography of MMP-2 and MMP-9 6
9. Statistical Methods 7
Ⅲ. RESULTS 8
A. Expression of EGFR in Human Hypopharyngeal Caner Tissue 8
B. Western blot in hypopharyngeal cancer tissue and RT-PCR and Western
blot in Human Hypopharyngeal Cancer Cells 11
C. Effect of EGF on proliferation of FaDu cells 13
D. Effect of EGF on colony dispersion 14
E. Effect of EGF on Wound Healing assay 15
F. Effect of EGFR on Cell Invasion 16
G. RT-PCR of MMP-2 and MMP-9 17
H. Zymography of MMP-2 and MMP-9 18
Ⅳ. DISCUSSION 19
Ⅴ. CONCLUSION 23
REFERENCES 24
국문요약 32MasterBackground and Objectives: The EGFR is a member of the erbB family of receptor tyrosine kinase proteins, which also includes HER2/neu (erbB2), HER3 (erbB3), and HER4 (erbB4). High levels of EGFR expression are correlated with poor prognosis and resistance to radiation therapy in a variety of cancers, mostly in head and neck squamous cell carcinoma (HNSCC). So we examined the expression of EGFR in hypopharyngeal cancer and the effect of EGF in progression of hypopharyngeal SCC in the hypopharyngeal SCC cell line (FaDu).
Materials and Methods: We performed immunohistochemical stains on 57 specimens of each normal mucosa and hypopharyngeal SCC with EGFR antibody. For Western blot, fresh normal and cancer tissue from the hypopharynx obtained from three patients were used. We performed RT-PCR and Western blot analyses of FaDu cells. Proliferative of the FaDu cells was assayed by counting the number of the cells after treatment by EGF of different concentration of 0, 10, 30 ng/㎖. Dispersion of the cells was observed by measuring the separation and morphologic changes of the cells after colony of FaDu cells was formed in the media and then was treated with EGF of 10ng/㎖ or 30ng/㎖ for 24 hours. Tumor cell invasiveness was assessed by the membrane invasion assay. RT-PCR and zymography were performed to examine the roles of matrix metalloproteinase-2(MMP-2) and MMP-9, as well as the relationship between EGF and MMPs in FaDu invasiveness.
Results: The positive rates of EGFR expression in hypopharyngeal SCC were 63.2%. EGFR staining was significantly correlated with pathologic stage (p<0.05). The increased expresssion of EGFR mRNA(RT-PCR) and protein (Western Blot) were detected in hypopharyngeal cancer tissue. Exogenous EGF significantly enhanced the growth of FaDu in a dose-dependent manner 30ng/㎖ (p<0.05). EGF markedly enhanced the invasiveness of cancer cells in a Transwell invasion chamber in a dose-dependent manner (p < 0.05). MMP expression was detected in the FaDu cells, and exogenous EGF enhanced the expression and its activity in the RT-PCR and zymogram analysis.
Conclusions: These results suggest that EGF may play an important role in the progression of hypopharyngeal cancer
New ESL Coordinator Ki-tae Kim, Ph.D., Joins Staff at U of M Crookston
No full textGeller, Jack; Tollefson, Elizabeth. (2008). New ESL Coordinator Ki-tae Kim, Ph.D., Joins Staff at U of M Crookston. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/221812
Convenio Específico de Donación entre la Facultad de Tecnología y Ciencias Aplicadas y Posco Argentina
No full textSavio, Carlos Humberto. Universidad Nacional de Catamarca. Facultad de Tecnología y Ciencias Aplicadas. Catamarca; Argentina.Sung Tae, Kim. Posco Argentina. Catamarca; Argentina
sj-tif-1-ear-10.1177_01455613231214705 – Supplemental material for Sudden Unilateral Visual Loss Due to an Onodi Cell Mucocele
No full textSupplemental material, sj-tif-1-ear-10.1177_01455613231214705 for Sudden Unilateral Visual Loss Due to an Onodi Cell Mucocele by Yong Jun Yoo, Gyu Tae Kim and Marn Joon Park in Ear, Nose & Throat Journal</p
DS_10.1177_0363546520908805 – Supplemental material for Evaluation of Anterolateral Ligament Healing After Anatomic Anterior Cruciate Ligament Reconstruction
No full textSupplemental material, DS_10.1177_0363546520908805 for Evaluation of Anterolateral Ligament Healing After Anatomic Anterior Cruciate Ligament Reconstruction by Dhong Won Lee, Jin Goo Kim, Hyun Tae Kim and Seung Ik Cho in The American Journal of Sports Medicine</p
Improvement of strength and water absorption of Interlocking Compressed Earth Bricks (ICEB) with addition of Ureolytic Bacteria (UB)
No full textInterlocking Compressed Earth Brick (ICEB) are cement stabilized soil bricks that allow for dry stacked construction. This characteristic resulted to faster the process of building walls and requires less skilled labour as the bricks are laid dry and lock into place. However there is plenty room for improving the interlocking bricks by increase its durability. Many studies have been conducted in order to improve the durability of bricks by using environmentally method. One of the methods is by introducing bacteria into bricks. Bacteria in brick induced calcite precipitation (calcite crystals) to cover the voids continuously. Ureolytic Bacteria (UB) was used in this study as a partial replacement of limestone water with percentage of 1%, 3% and 5%. Enrichment process was done in soil condition to ensure the survivability of UB in ICEB environment. This paper evaluates the effect of UB in improving the strength and water absorption properties of ICEB and microstructure analysis. The results show that addition of 5% UB in ICEB indicated positive results in improving the ICEB properties by 15.25% in strength, 14.72% in initial water absorption and 14.68% reduction in water absorption. Precipitation of calcium carbonate (CaCo3) in form of calcite can be distinguish clearly in microstructure analysis
Korean Folk Tales 16
No full textA very pleasing book. The Golden Axe is Aesopic (though never acknowledged here as such) and nicely elaborated and illustrated for this culture. Dokbo lives in a village tucked away in the mountains. The artist misses, I believe, that Dokbo lost the head of his axe. Yunbo does not hear the whole story and so proceeds falsely with the old man water spirit. Yunbo falls in trying to grasp the spirit--and gets nothing but a cold.Language note: Bilingual: Korean/EnglishMark C.K. Setto
- …
