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    Biofilm as an adaptation strategy to extreme conditions

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    Biofilm is the most successful and widely distributed form of life on earth, it is not simply structured collections of cells attached to surfaces but is a dynamic complex biological system able to respond to environmental changes. The biofilm characteristics make it unique and central to microbial evolution and adaptation. The ability to establish biofilms is a key trait for microorganisms growing in extreme environments like extreme temperature, high radiation, acidic or alkaline pH values, heavy metal pollution, and high salinity. In this article, we report the main features of biofilm and how these characteristics make biofilms a successful survival strategy in extreme conditions. All aspects examined in this article help to explain why biofilms are a successful survival strategy in extreme conditions and why the ability to establish biofilms is a key trait for microorganisms growing in extreme environments

    The outer membrane glycolipids of bacteria from cold environments: Isolation, characterization, and biological activity

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    Lipopolysaccharides (LPSs) are the main components of the external leaflet of the outer membrane of Gram-negative bacteria. Microorganisms that colonize permanently or transiently cold habitats have evolved an array of structural adaptations, some of which involve components of bacterial membranes. These adaptations assure the perfect functionality of the membrane even at freezing or sub-freezing growth temperatures. This review summarizes the state-of-the-art information concerning the structural features of the LPSs produced by cold-adapted bacteria. The LPS structure has recently been elucidated from species mainly belonging to Gammaproteobacteria and Flavobacteriaceae. Although the reported structural heterogeneity may arise from the phylogenetic diversity of the analyzed source strains, some generalized trends can be deduced. For instance, it is clear that only a small portion of LPSs displays the O-chain. In addition, the biological activity of the lipid A portion from several cold-adapted strains is reported

    Cell-wall associated polysaccharide from the psychrotolerant bacterium Psychrobacter arcticus 273-4: isolation, purification and structural elucidation

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    In this paper, the structure of the capsular polysaccharide isolated from the psychrotolerant bacterium Psychrobacter arcticus 273-4 is reported. The polymer was purified by gel filtration chromatography and the structure was elucidated by means of one- and two-dimensional NMR spectroscopy, in combination with chemical analyses. The polysaccharide consists of a trisaccharidic repeating unit containing two residues of glucose and a residue of a N,N-diacetyl-pseudaminic acid
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