1,720,961 research outputs found
THE UNFOLDED PROTEIN RESPONSE: A LINK BETWEEN ENDOMETRIOID OVARIAN CARCINOMA AND ENDOMETRIOSIS
Full text linkLo scopo del seguente lavoro è di analizzare il profilo di attivazione dei geni legati al pathway dell’Unfolded Protein Response (UPR) nel carcinoma endometrioide dell’ovaio e di valutare il suo possibile coinvolgimento nella trasformazione neoplastica dell’endometriosi.
Lo studio è stato eseguito utilizzando diversi campioni istologici: carcinoma endometrioide dell’ovaio, tessuto ovarico sano, cisti endometriosica, endometrio eutopico provenienti da pazienti con endometriosi e da pazienti con endometrio sano. Da tutti i campioni è stato estratto l’RNA e sintetizzato il cDNA utilizzando la trascrizione inversa. Il cDNA è stato utilizzato per i saggi di espressione quantitativa dei geni, tramite Real Time PCR, con analisi dei geni appartenenti al pathway dell’UPR. I campioni sono stati divisi in tre gruppi: patienti con endometriosi (n=6), pazienti sane (n=6) e pazienti con carcinoma endometrioide dell’ovaio (n=6).
L’analisi statistica effettuata è il t-test, con analisi delle differenze statistiche tra i dati provenienti da pazienti sane (CTRL) e pazienti affette da endometriosi (Ectopic e Eutopic) e pazienti affette da carcinoma endometrioide dell’ovaio (CA).
Abbiamo in primo luogo analizzato la differente espressione del pathway dell’UPR nel carcinoma endometrioide dell’ovaio, comparandolo al tessuto ovarico sano e abbiamo dimostrato un’alterata espressione dei geni dell’UPR nelle pazienti tumorali. In secondo luogo, abbiamo analizzato l’espressione genica dell’UPR nel carcionma endometrioide ovarico, comparandola all’endometrio sano di pazienti sane e di pazienti affette da endometriosi.
Il nostro studio mostra una graduale riduzione dell’espressione del gene XBP1 nell’endometriosi, caratterizzata da intensa infiammazione e nel carcinoma endometrioide dell’ovaio, valorizzando l’ipotesi che XBP1 possa rappresentare un marker di trasformazione neoplastica.
In conclusione XBP1 ha un’alta espressione nell’endometrio sano, un tessuto costitutivamente secretivo, e poi gradualmente riduce la sua espressione nell’endometriosi e, in maniera più accentuata, nel carcinoma ovarico.
Comprendere questi meccanismi potrebbe rappresentare uno step importante per una migliore definizione della patogenesi tumorale e per lo sviluppo in futuro di terapie geniche mirate.The present study aims to analyze the activation profile of Unfolded Protein Response (UPR) related genes in endometriod ovarian carcinoma and to assess its possible involvement in the neoplastic transformation from endometriosis.
The study was performed using different histological samples: endometrioid carcinoma of the ovary, healthy ovary, endometriosis cysts, eutopic endometrium from patients with endometriosis and healthy endometrium. From all the samples RNA was extracted and cDNA synthesis was performed by reverse transcription. cDNA was used for quantitative gene expression assays, made by Real Time PCR, analyzing genes belonging to the UPR pathway. Samples were divided into three groups: patients with endometriosis (n = 6), healthy patients (n = 6) patients with ovarian endometrioid carcinoma (n = 6).
Statistical analysis performed was a t - test, testing the statistical differences, between data means from healthy patients (CTRL) and groups of patients with endometriosis (Ectopic and Eutopic) and patient with endometrioid carcinoma of the ovary (CA).
We started analyzing the different expression of UPR pathway in endometrioid ovarian carcinoma compared to healthy ovary and we demonstrated an altered UPR gene expression in patients affected by endometrioid ovarian carcinoma, compared to healthy ovary. As a second step, we decided to analyze the UPR pathway genetic expression in the endometrioid ovarian carcinoma compared to the endometrium of healthy patients and of endometriosis patients. Our study shows a gradual reduction of XBP1 expression in endometriosis, characterized by intense inflammation, and endometrioid ovarian carcinoma, thus strengthening the hypothesis of XBP1 as a marker of neoplastic transformation. Conclusively XBP1s has a high basic expression in healthy endometrium, being a secretive tissue, then gradually decreases in endometriosis and to a higher degree, in ovarian carcinoma.
Understanding these mechanisms could represent an important step, for a better definition of cancer pathogenesis, and also in the future, for the development of customized therapies
Laparoscopic treatment of sclerosing stromal tumor of the ovary in a woman with Gorlin-Goltz Syndrome: a case report and review of the literature
No full textGorlin-Goltz syndrome is a rare hereditary multisystemic disease. Multiple basal cell carcinomas, odontogenic keratocysts and skeletal abnormalities are the main clinical manifestations of the syndrome, but several organs can be involved. Moreover this condition is associated with the development of various benign and malignant tumors, even in the genital tract. This report describes a rare association between Gorlin-Goltz syndrome and the sclerosing stromal tumor of the ovary. Since the ultrasounds and magnetic resonance pattern of this tumor can be similar to those of a malignant neoplasm, a prompt surgical intervention and histological confirmation of diagnosis is mandatory. However this is a benign lesion and thus can be approached with a laparoscopic fertility sparing surgery. Gynecologists should be aware of this possible association, in order to provide an appropriate counseling to these women, and to perform a fertility sparing laparoscopic approach, wherever possible
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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