1,721,004 research outputs found
Core-shell functional nanospheres for oligonucleotide delivery. III. Stealth nanospheres
No full textPolymthyl methacrylate-based stealth and functional nanospheres, specifically designed for the reversible adsorption of oligonucleotides (ODN), were prepared by emulsion polymerization of methyl methacrylate in the presence of an ionic comonomer, namely a quaternary ammonium salt of 2-(dimethylamino)ethyl methacrylate, and a nonionic comonomer, namely a poly(ethylene glycol) methacrylate. The nanosphere size is substantially affected by the amount of both the nonionic and ionic comonomers. By appropriately adjusting the concentrations of the ionic and nonionic comonomers, the quaternary ammonium group and PEG chain surface densities can be finely tuned. Accordingly, a great variety of core-shell-type nanospheres, able to bind ODN and to induce dysopsonic effect, can be obtained
PKA DETERMINATION OF THE DEOXYDINUCLEOTIDE MONOPHOSPHATE CA AND OF THE CORRESPONDING HEXANUCLEOTIDE CACACA BY INVERSE-DETECTED C-13 NMR
No full textThe pK(a) values of adenine N1 and cytosine N3 in the non-self complementary title compounds have been measured by C-13 NMR, using an inverse probe. in the deoxydinucleotide adenine N1 has pK(a) = 2.52, markedly smaller than that of cytosine N3 for which pK(a) = 4.42. In the hexanucleotide the pK(a) values of adenine N1 and cytosine N3 are much closer, being 3.97 and 4.67, respectively
Nanoparticles for delivery of a pharmacologically active agent (United Kingdom Patent application)
No full textField of Invention.
The present invention relates to core-shell nanoparticles, processes for preparing them, and their use as carriers able to reversibly bind and deliver pharmacologically active substances, in particular nucleic acids, including natural and modified (deoxy)ribonucleotides (DNA, RNA), oligo(deoxy)nucleotides (ODNs) and proteins into cells
Highly efficient cellular uptake of c-myb antisense oligonucleotides through specifically designed polymeric nanospheres
No full textFunctional polymeric nano/microparticles for protein and DNA vaccine delivery.
No full textThe use of particulate polymeric carriers holds great promise for the development of effective and affordable DNA and protein subunit vaccines. Rational development of such vaccine formulations requires a detailed understanding of their physico-chemical properties, cell-free and in vitro behaviour, in addition to particle uptake and processing mechanisms to antigen presenting cells capable of stimulating safe and effective immune responses. We here provide an overview on functional polymeric nano- and micro-particles designed for surface adsorption of proteins and DNA antigens currently under investigation for the formulation of new vaccines, including comments on their preparation method, antigen delivery strategy, cell-free and in vitro behaviour. In addition, we focus on their influence in activating antigen-specific humoral and/or cellular immune responses and on their potential for the development of new vaccines
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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