1,721,166 research outputs found
Editorial: Association Between Human Cancers and Small DNA Tumor Viruses
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Molecular approach in human tumor investigation: oncogenes, tumor suppressor genes and DNA tumor polyomaviruses (review).
No full textMolecular analysis are useful for diagnosis, prognosis and follow-up of the patients, as well as for addressing therapeutic choices. Most of the molecular methods are based on the analysis of nucleic acids. The DNA and RNA methodologies of routine applicability include Southern and Northern hybridizations and polymerase chain reaction (PCR) techniques. Southern blot hybridization recognizes major DNA rearrangements, and detection of oncogenic viral sequences present in high copy number, whereas PCR-based methods allow the detection of gross chromosomal modifications, fine gene alterations and low amount of tumor virus footprints. PCR techniques also allow the analysis of the partially degraded nucleic acids from formalin-fixed paraffin-embedded tissues. We present an overview of the use of molecular techniques for the analysis, diagnosis, prognosis and follow-up of neoplastic diseases, using examples from our experience in both leukemias and solid tumors
Observer-Based Control of Position and Tension for an Aerial Robot Tethered to a Moving Platform
No full textIn this letter, we address a challenging version of the problem of controlling tethered aerial vehicles (also known as UAV, MAV, and UAS) by considering the aerial robot linked to a generic and independently moving platform. We solve the exact tracking control problem for both the 3-D position of the robot (either absolute or with respect to the platform) and the tension along the link. To achieve this goal, we prove some fundamental system properties, useful to design a nonlinear controller, such as differential flatness and dynamic feedback linearizability. To close the control loop, a set of minimal and standard sensors is proposed. Then, we show that it is possible to retrieve the full system state from those sensors by means of nonlinear measurements transformations and a bank of low-dimension estimators based on the nonlinear high gain observer. The ability of the proposed observer-controller method is tested by extensive numerical simulations spanning many nonideal conditions
Some theoretical aspects of the mechanism of action of benzhydrazone, an inhibitor of glycosylation in herpes simplex virus
No full textSome hypotheses of the mechanism of action of benzhydrazone, a selective inhibitor of herpes simplex virus glycoprotein biosynthesis, are presented in this paper. Comparative analysis of different models indicates that the drug may interfere with herpes simplex virus DNA. Thus, benzhydrazone seems to act like a bifunctional alkylating agent
Restriction Endonuclease Patterns of Herpes Simplex Virus DNA: Application to Diagnosis and Molecular Epidemiology
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
MICROSATELLITE DNA AS A MARKER FOR MAPPING OF LOCI LINKED TO CRANIOFACIAL MALFORMATIONS
No full textCleft Lip with or without cleft palate (CL +/- P) is one of most common congenital orofacial anomaly, with a frequency of 1/700-1/1,000 live births among Caucasians. This embryopathy is due to a failure of nasal processes and palatal shelves fusion. Approximately 20 % of patients have a positive family history of CL +/- P, and thus genetic factors are thought to be important in its etiology. Four different chromosomal regions, 6p23-25, 2p13, 17q21.1 and 1q21, have been claimed to contain a CL +/- P locus. In our study we investigated the possible CL +/- P locus of chromosome 6p23. To this purpose we selected a group of markers, which map on this chromosome region, to analyse a large sample of families. Twentyone families characterized by the presence of at least two affected CL +/- P individuals, arising from north-eastern Italy, were enrolled in this study. DNA was analyzed with five highly informative PCR markers close to the putative CL +/- P locus: 1 VNTR factor 13A, and 4 dinucleotide repeats at loci EDN1, D6S89, D6S109, D6S105. Our results demonstrate that nonsyndromic familiar CL +/- P is heterogeneous; these data are in agreement with the idea of a model of a dominant major gene modified by additional genetic and/or enviromental factors. So it is possible that different chromosomal region are involved in etiology of the disease. Our linked families showed an autosomal dominant inheritance with incomplete penetrance. Finally, CL +/- P locus maps on 6p23 very close to, or at the microsatellite marker D6S89
Mappatura di tre diversi loci sinciziali presenti nel DNA dei virus herpes simplex di tipo1
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