1,720,993 research outputs found
Biotechnical methods for the production of L-amino acids
No full textLeuchtenberger W, Kircher M, Thierbach G. Biotechnical methods for the production of L-amino acids. Swiss Biotech. 1986;4(1):20-22
TRANSFORMATION OF SPHEROPLASTS AND PROTOPLASTS OF CORYNEBACTERIUM-GLUTAMICUM
No full textTHIERBACH G, SCHWARZER A, Pühler A. TRANSFORMATION OF SPHEROPLASTS AND PROTOPLASTS OF CORYNEBACTERIUM-GLUTAMICUM. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 1988;29(4):356-362
Versatile low-copy-number plasmids for temperature-inducible overexpression of bacterial genes in Escherichia coli
No full textGötting C, Thierbach G, Pühler A, Kalinowski J. Versatile low-copy-number plasmids for temperature-inducible overexpression of bacterial genes in Escherichia coli. BIOTECHNIQUES. 1998;24(3):362-+
Cloning of a DNA fragment from Corynebacterium glutamicum conferring aminoethyl cysteine resistance and feedback resistance to aspartokinase.
No full textThierbach G, Kalinowski J, Bachmann B, Pühler A. Cloning of a DNA fragment from Corynebacterium glutamicum conferring aminoethyl cysteine resistance and feedback resistance to aspartokinase. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 1990;32(4):443-448
Phosphoenolpyruvate carboxylase in Corynebacterium glutamicum is dispensable for growth and lysine production.
No full textPeters-Wendisch P, Eikmanns BJ, Thierbach G, Bachmann B, Sahm H. Phosphoenolpyruvate carboxylase in Corynebacterium glutamicum is dispensable for growth and lysine production. FEMS Microbiology Letters. 1993;112:269-274
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum
No full textSCHAEFER A, Tauch A, JAEGER W, Kalinowski J, THIERBACH G, Pühler A. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene. 1994;145(1):69-73.Here we describe small mobilizable vectors based on the Escherichia coli plasmids pK18 and pK19. We combined the useful properties of the pK plasmids (e.g., multiple cloning site, lacZ alpha fragment, sequencing with M13 primers) with the broad-host-range transfer machinery of plasmid RP4 and a modified sacB gene from Bacillus subtilis. The new pK derivatives can be transferred by RP4-mediated conjugation into a wide range of Gram(-) and Gram(+) bacteria, and should facilitate gene disruption and allelic exchange by homologous recombination. As an application example, the generation of a defined deletion of the hom-thrB genes in the chromosome of the Gram(+) bacterium Corynebacterium glutamicum glutamicum is presented
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Plasmids in Corynebacterium glutamicum and their molecular classification by comparative genomics
No full textTauch A, Pühler A, Kalinowski J, Thierbach G. Plasmids in Corynebacterium glutamicum and their molecular classification by comparative genomics. JOURNAL OF BIOTECHNOLOGY. 2003;104(1-3):27-40.Endogenous plasmids and selectable resistance markers are a fundamental prerequisite for the development of efficient recombinant DNA techniques in industrial microorganisms. In this article, we therefore summarize the current knowledge about endogenous plasmids in amino acid-producing Corynebacterium glutamicum isolates. Screening studies identified a total of 24 different plasmids ranging in size from 2.4 to 95 kb. Although most of the C glutamicum plasmids were cryptic, four plasmids carried resistance determinants against the antibiotics chloramphenicol, tetracycline, streptomycin-spectinomycin, and sulfonamides. Considerable information is now available on the molecular genetic organization of 12 completely sequenced plasmid genomes from C. glutamicum. The deduced mechanism of plasmid DNA replication and the degree of amino acid sequence similarity among replication initiator proteins was the basis for performing a classification of the plasmids into four distinct C. glutamicum plasmid families. (C) 2003 Elsevier B.V. All rights reserved
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