305,307 research outputs found
Porcine odorant-binding protein: structural stability and ligand affinities measured by Fourier-transform infrared spectroscopy and fluorescence spectroscopy
No full textInfrared spectra show that the binding of the odorants 2-isobuthyl-3-mrthoxypyrazine (PYR) and 3,7-dimethyl-1-octanol (DMO) stabilises the tertiary structure of porcine OBP-I against thermal denaturation. The fluorescence emission spectrum of the single tryptophan shows a lambda(max) at 337 nm, indicating that the residue is not directly exposed to the solvent. Tryptophan does not appear to be involved in the odorant binding process and it is not accessible to the fluorescence quenchers NaI, CsCl and acrylamide. The binding of the fluorescent dye 1-aminoanthracene (1-AMA), a strong ligand, does not modify the tryptophan fluorescence spectrum. In contrast, the lambda(max) of 1-AMA bound to OBP-I is shifted from 537 to 481 nm, with a lambda(max) intensity increase by a factor of 80. Bound 1-AMA is displaced by odorant molecules in competitive binding assays and can be employed in simple and rapid binding assay, avoiding the use of radioactive ligands. The Scatchard plot shows that 1-AMA binds to OBP-I with a dissociation constant of 1.3 mu M and an equimolar stoichiometry. (C) 1999 Elsevier Science B.V. All rights reserved. RI Tanfani, Fabio/F-1441-201
Thymoquinone, a potential therapeutic agent of Nigella sativa, binds to site I of human serum albumin.
No full textThymoquinone, a potential therapeutic agent of Nigella sativa, binds to site I of human serum albumin
G. Lupidi a, 1, A. Scire b, 1, E. Camaioni c, K.H. Khalife a, G. De Sanctis a, F. Tanfani b, E. Damiani b, ,
a Dipartimento di Biologia M.C.A., Università degli Studi di Camerino, Camerino, Italy
b Dipartimento di Biochimica, Biologia e Genetica, Università Politecnica delle Marche, Ancona, Italy
c Dipartimento di Chimica e Tecnologia del Farmaco, Università degli Studi di Perugia, Perugia, Italy
Abstract
Thymoquinone (TQ) is the main constituent of Nigella sativa essential oil which shows promising in vitro and in vivo antineoplastic growth inhibition against various tumor cell lines. Because of the increasing interest to test it in pre-clinical and clinical researches for assessing its health benefits, we here evaluate the interactions between TQ and human serum albumin (HSA), a possible carrier of this drug in vivo. Binding to HSA was studied using different spectroscopic techniques. Fourier transform infrared (FT-IR) and circular dichroism (CD) spectroscopies suggest that the association between TQ and HSA does not affect the secondary structure of HSA. Using fluorescence spectroscopy, one mole of TQ was found to bind one mole of HSA with a binding constant of 2.39 ± 0.2 104 M−1. At 25 °C (pH 7.4), van’t Hoff’s enthalpy and entropy that accompany the binding were found to be −10.24 kJ/mol−1 and 45 J/mol−1 K−1 respectively. The thermodynamic analysis of the TQ-HSA complex formation shows that the binding process is enthalpy driven and spontaneous, and that hydrophobic interactions are the predominant intermolecular forces stabilizing the complex. Furthermore, displacement experiments using warfarin and ibuprofen indicate that TQ could bind to site I of HSA, which is also in agreement with the results of the molecular modeling study
A simulation model to evaluate the impact of prioritisation system on surgical waiting list management
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Enrico Tanfani - recto
No full textBotanico: Tanfani, Enrico (1848-1892).
Assistente al museo botanico di Firenze e professore di scienze naturali nel reale liceo militare.
Titolo ed estremi cronologici riportati manoscritti sul recto, dove compaiono anche le note: 1899; prest. Eug. Baroni. da Racc. [Raccolta] Caruel.
Montata su cartoncino 117 x 66 mm.
1 fotografia : aristotipo ; 86 x 57 mm.
Vai alla scheda bibliografica: https://galileodiscovery.unipd.it/discovery/fulldisplay?context=L&vid=39UPD_INST:VU1&search_scope=MyInst_and_CI&tab=Everything&docid=alma99001524713020604
Assessing migration health care implications: a systematic review of literature - Collana Percorsi di Scienze Economiche e Sociali
No full textAssessing migration health care implications: a systematic review of literature - Collana Percorsi di Scienze Economiche e Sociali
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