493 research outputs found
Concomitant inactivation of <i>perRA</i> and <i>perRB</i>.
No full text(A) Schematic representation of the double perRAperRB mutant construction. PerRA (LIMLP_10155) was inactivated by allelic exchange in the transposon perRB mutant. The kanamycin (Km) and spectinomycin (Spc) resistance cassettes inactivating perRB and perRA, respectively, are indicated. (B) Production of PerRA in the WT, in the single perRA and perRB mutants and in the double perRAperRB mutant strains. L. interrogans strains were cultivated in EMJH medium at 30°C until the logarithmic phase and lyzed by sonication in 25 mM Tris pH 7.5, 100 mM KCl, 2 mM EDTA, 5 mM DTT, with a protease inhibitors cocktail (cOmplete Mini EDTA-free, Roche). 10 μg of total lyzates were resolved on a 15% SDS-PAGE and transferred on nitrocellulose membrane. PerRA was detected by immunoblot using a 1/2000 antibody dilution as described previously (Kebouchi et al., J Biol Chem. 2018;293(2):497–509. doi:10.1074/jbc.M117.804443). (C) Growth of stationary phase-adapted WT and perRAperRB mutant strains. L. interrogans WT (black circles) and perRAperRB mutant (pink squares) were cultivated in EMJH medium at 30°C until late stationary phase (7 days after the entry in the stationary phase) and used to inoculate EMJH medium. Bacteria were then cultivated at 30°C and growth was assessed by absorbance at 420 nm. Data are means and standard errors of three independent biological experiments. (TIF)</p
Distribuzione di contaminanti nei sedimenti marini superficiali dell'area di Capo Vaticano (Calabria, Italia)
No full textOptimization of drugs delivery routes through location routing problem (Lrp): A case study
No full textThe location-routing problem (LRP) plays a critical role in logistics system optimization where companies need to set up a proper design of operation strategies to fulfil the demand for product delivery. This paper analysed an efficient products distribution of a delivery drugs Italian company through the development of LRP analysis. Thus, due to a reduction of company sales volume, a delivery routes re-planning was investigated moving from a logistics plan that involved the use of the main warehouse with replenishment at intermediate one to a new configuration with only the main warehouse. The analysis is based on the identification of the ideal location of the main warehouse by optimizing the delivery routes of goods to meet customers’ demands. Optimal solutions are found according to the integration of the facility location and the vehicle-routing problems (VRP) aimed at minimizing the total logistic system cost
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