51 research outputs found
Molecular Pharmaceutical Farming in Transgenic Plants
Full text linkPlants are a tremendous source for the discovery of new products of medicinal value for drug development. Today several distinct chemicals derived from plants are important drugs currently used in one or more countries in the world. Many of the drugs sold today are simple synthetic modifications or copies of the naturally obtained substances. The evolving commercial importance of secondary metabolites has in recent years resulted in a great interest in secondary metabolism, particularly in the possibility of altering the production of bioactive plant metabolites by means of Plant Biotechnology or Green Gene technology and tissue or cells culture technology. Plant Biotechnology is possible tool for both studying and producing plant secondary metabolites which is called Molecular farming. Different strategies, using Plant Molecular farming system, have been extensively studied to improve the production of plant chemicals. The development of genetically transformed plant tissue cultures and mainly of roots transformed by Agrobacterium rhizogenes (hairy roots), is a key step in the use of in vitro cultures for the production of secondary metabolites.
Hairy roots are able to grow fast without phytohormones, and to produce the metabolites of inserted genes. The conditions of transformation (nature and age of the explants, bacterial strain, bacterial density, and the protocol of infection) deeply influence the frequency of the transformation events as well as the growth and productivity of the hairy roots. Then optimization of the culture parameters (medium constituents, elicitation by biotic or abiotic stress) may enhance the capability of the hairy roots to grow fast and to produce valuable compounds.
The focus of the present paper is the review of Hairy roots production of Plant Transformation and tissue culture technology for the production of some important plant pharmaceuticals. Also will explain the recent results in our research of Transgenic Plant with good hairy roots formation and high Catechin in Theobroma cacao and in Eucaria Gambir. So far, There is no report concerning gene transfer by Agrobacterium rhizogenes and Hairy roots induction in both plants.
Keywords : Molecular Farming,, Review Hairy Roots A. rhizogenese, Secondary Metabolite Catechin, T.cacao, Eucaria gambir
Bioteknologi Tepat Guna : Virgin Coconut Oil(VCO) Untuk Pembangunan Industri Pedesaan dan meningkatkam Kesehatan Masyarakat
No full text38 hal,;ill,;21 c
Antimicrobial Properties and Lactase Activities from Selected Probiotic Lactobacillus brevis Associated With Green Cacao Fermentation in West Sumatra, Indonesia
No full textDETERMINATION OF CALCIUM (Ca) AND MAGNESIUM (Mg) CONTENT IN CACAO (Theobroma cacao Linn) FERMENTATION AND NON FERMENTATION BY SPECTROPHOTOMETRY
Full text link ABSTRACT Cacao beans contain many kinds of mineral, magnesium (Mg), calcium (Ca), Zinc (Zn), Phosphor (P) and etc. This study investigated magnesium (Mg) and calcium (Ca) in fermentation and non fermentation cacao beans by atomic absorption spectrophotometry. Mg and Ca, content in non fermentation cacao beans of green and red variety are 453 µg/g, 466 µg/g, and 491 µg/g, 445 µg/g. Mg and Ca, contents in fermentation cacao beans of green and red variety are, 596 µg/g, 528 µg/g, and 554 µg/g, 505 µg/g. Fermentation make magnesium (Mg) and calcium (Ca) content increase significantly. Keywords : Theobroma cacao Linn, fermentation, spectrophotometry.</jats:p
BIOTEKNOLOGI DASAR DAN BAKTERI ASAM LAKTAT ANTIMIKROBIAL
Full text linkBuku ini juga merupakan output dari proyek penelitian hibah PascaSarjana 2 tahun berturut turut dengan kontrak tahap I No: 394/SP2H/PL/Dit.Litabmas/IV/2011 dan Kontrak tahap II No: 004/UN.16/PL/MT-HB-PC/2012, dilanjutkan dengan hibah kompetensi multiyears yang berakhir tahun 2016. Ucapan terima kasih juga untuk mahasiswa S2 bioteknologi, Dani Prasetiyo yang juga telah membantu editing. Kami berharap buku ini dapat bermanfaaat bagi mahasiswa sebagai sumber bahan bacaan dan bahan ajar kuliah Bioteknologi dan Teknik Penelitian Probiotik. Penulis berharap agar dimasa mendatang dapat ditemukan diversity dari Probiotik lokal Sumatra Barat dan Probiotik potensial baru dari Fermentasi Pangan Lokal sebagai Mikroba baik untuk menjaga kesehatan total terutama usus manusia dan hewan ternak serta applikasi Bakteriosin sebagai Food Preservatif Alami. 
TOPIK PENEMUAN BAKTERIOSIN BARU SEBAGAI ANTIMIKROBA DARI BAKTERI ASAM LAKTAT FERMENTASI DADIAH SUMATRA BARAT
No full text- …
