2,693 research outputs found
Episode 21: Matt Eicheldinger: Educator Turned Author
No full textMatt Eicheldinger, B.A. \u2709, M.A. \u2712 is an educator who used stories from his life to motivate his middle school students. When he found that not only were these stories effective, when written down, they inspired even the most reluctant of readers. This put Matt down a path of becoming a published author. In 2021, he launched a Kickstarter campaign to self-publish Matt Sprouts and The Curse of Ten Broken Toes. When the book became a hit, he was able to sign with an agent who quickly sold Matt Sprouts to a publisher. Matt shares how he became interested in being an educator, how he navigated the process of becoming a published author, and his future plans for more books
Vibrio cholerae (WT w/plasmid)
No full textWT Vibrio carrying pMMb67 plasmid without GFP-MreB<strong>Tilt Series Date:</strong> 2010-12-12</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> TRH7000</p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (0.0°, 0.0°), step: °, constant angular increment, dosage: 0.0 eV/Ų, defocus: 0.0 μm, magnification: 0x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Sample Preparation:</strong> No induction of GFP-MreB</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2010-12-12-25</p>10dec12c_at1_028_part.rec, Reconstruction (Pixel Size 0.0 nm), 838.9 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2010-12-12-25/3dimage_43379/10dec12c_at1_028_part.rec"
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Escherichia coli
No full textRaw data files of Escherichia coli<strong>Tilt Series Date:</strong> 2015-03-20</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> MG1655 sacculi</p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 110.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-03-20-12</p>032015_mg1655_a220006.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-03-20-12/rawdata/032015_mg1655_a220006.mrc"
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032015_mg1655_a220006_full.rec, Reconstruction (Pixel Size 1.92 nm), 445.4 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-03-20-12/3dimage_53292/032015_mg1655_a220006_full.rec"
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Vibrio cholerae (GFP-MreB)
No full textStrain TRH7000 from Maria Sandkvist<strong>Tilt Series Date:</strong> 2010-10-10</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> TRH7000</p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (0.0°, 0.0°), step: °, constant angular increment, dosage: 0.0 eV/Ų, defocus: 0.0 μm, magnification: 0x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> raptor</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2010-10-10-1</p>10oct10a_at1_004.ali, Tilt Series (Pixel Size 0.0 nm), 253.8 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2010-10-10-1/rawdata/10oct10a_at1_004.ali"
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10oct10a_at1_004.rec, Reconstruction (Pixel Size 0.0 nm), 262.1 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2010-10-10-1/3dimage_43307/10oct10a_at1_004.rec"
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Escherichia coli
No full textRaw data files of Escherichia coli<strong>Tilt Series Date:</strong> 2015-03-20</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> MG1655 sacculi</p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 110.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-03-20-13</p>032015_mg1655_a22_1.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-03-20-13/rawdata/032015_mg1655_a22_1.mrc"
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032015_mg1655_a22_1_full.rec, Reconstruction (Pixel Size 1.92 nm), 445.4 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-03-20-13/3dimage_53291/032015_mg1655_a22_1_full.rec"
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Escherichia coli
No full textRaw data files of Escherichia coli<strong>Tilt Series Date:</strong> 2015-03-21</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> MG1655 sacculi</p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x. </p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> Raptor</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-03-21-7</p>032115_purified_actomyosin0007.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-03-21-7/rawdata/032115_purified_actomyosin0007.mrc"
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032115_purified_actomyosin0007_part121_22.rec, Reconstruction (Pixel Size 1.92 nm), 445.4 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-03-21-7/3dimage_53298/032115_purified_actomyosin0007_part121_22.rec"
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E. coli Sacculi +A22 (MG1655)
No full textRaw data files of E. coli Sacculi +A22 (MG1655)<strong>Tilt Series Date:</strong> 2015-04-10</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> MG1655 sacculi</p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Sample Preparation:</strong> Cells were treated for one hour with 10ug/ml A22 until they were rounded and lemon-shaped prior to sacculus purification.</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-04-10-32</p>041015_mg1655_a22_atlas3Tile01.mrc, Tilt Series (Pixel Size 0.48 nm), 171.0 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-04-10-32/rawdata/041015_mg1655_a22_atlas3Tile01.mrc"
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E. coli Sacculi (MG1655)
No full textRaw data files of E. coli Sacculi (MG1655)<strong>Tilt Series Date:</strong> 2015-04-10</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Escherichia coli</p>
<strong>Strain:</strong> MG1655 sacculi</p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> raptor</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-04-10-5</p>041015_mg1655_atlas1_tomo0001.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-04-10-5/rawdata/041015_mg1655_atlas1_tomo0001.mrc"
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041015_mg1655_atlas1_tomo0001_part121_6.rec, Reconstruction (Pixel Size 1.92 nm), 445.4 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2015-04-10-5/3dimage_53923/041015_mg1655_atlas1_tomo0001_part121_6.rec"
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B subtilis bMD204
No full textmreB-40aa-sfGFP sfGFP-11aa-mbl::spec ΔponA::cat"dual-tagged" strain, where mreB is C-terminally tagged and includes a 40 aa linker, while mbl is N-terminally tagged and uses an 11 aa linker.<strong>Tilt Series Date:</strong> 2013-11-15</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Bacillus subtilis</p>
<strong>Strain:</strong> bMD204</p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> UCSFTomo</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Collaborators and Roles:</strong> Ethan Garner and Mike Dion</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-11-15-10</p>111510_bs2040010.mrc, Tilt Series (Pixel Size 0.48 nm), 1.7 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2013-11-15-10/rawdata/111510_bs2040010.mrc"
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Vibrio cholerae (WT w/plasmid)
No full textWT Vibrio carrying pMMb67 plasmid without GFP-MreB<strong>Tilt Series Date:</strong> 2010-12-12</p>
<strong>Data Taken By:</strong> Matt Swulius</p>
<strong>Species / Specimen:</strong> Vibrio cholerae</p>
<strong>Strain:</strong> TRH7000</p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (0.0°, 0.0°), step: °, constant angular increment, dosage: 0.0 eV/Ų, defocus: 0.0 μm, magnification: 0x. </p>
<strong>Microscope:</strong> Caltech Polara</p>
<strong>Acquisition Software:</strong> Undefined</p>
<strong>Upload Method:</strong> webload</p>
<strong>Processing Software Used:</strong> raptor</p>
<strong>Sample Preparation:</strong> No induction of GFP-MreB</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2010-12-12-27</p>10dec12c_at1_030_part.rec, Reconstruction (Pixel Size 0.0 nm), 838.9 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2010-12-12-27/3dimage_43381/10dec12c_at1_030_part.rec"
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