187,042 research outputs found
MSC-derived exosomes inhibit proliferation and migration of SVEC cells <i>in vitro</i>.
No full text<p>(A) 2.0×10<sup>3</sup> SVEC cells were incubated with the conditioned media from 4T1 cells that were treated with various concentrations of MSC-derived exosomes or carrier control (PBS). Cell proliferation rates were determined by an EZ-Cytox cell viability assay kit. (B) SVEC cells transwell migration assay was performed in the presence of the conditioned media from 4T1 cells that were treated with various concentrations of MSC-derived exosomes or carrier control (PBS) in the lower chambers. Serum-starved SVEC cells were added to the upper chamber and incubated for 24 h to allow cell migration through the membrane. The membranes were stained with crystal violet and cell migration was analyzed by Image J. (C) SVEC cells were scratched and incubated with the conditioned media from 4T1 cells stimulated with MSC-derived exosomes (100 μg/ml) or vehicle control (PBS) for 24 h. In order to neutralize VEGF derived from 4T1 cells, anti-VEGF antibodies (20 μg/ml) were added to the conditioned media. Photographs were taken immediately and 24 h after wounding (data not shown) and analyzed by Studio Lite, version 1.0. (D) SVEC cells were serum-starved for 24 h and 2×10<sup>4</sup> SVEC cells were seeded in a Matrigel-coated well. The cells were treated with conditioned media collected from 4T1 cells stimulated with MSC-derived exosomes (100 μg/ml) or carrier control (PBS) for 24 h and viewed under a microscope. The values are presented as the mean ± SD; n = 3 for each group. Significant differences were evaluated using an unpaired two-tailed Student's t-test. *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001 compared with control.</p
Manipulated wettability of a superhydrophobic quartz crystal microbalance through electrowetting
No full textThe liquid phase response of quartz crystal microbalances (QCM) with a thin coating (~9 µm) of epoxy resin with and without a carbon nanoparticles top layer is reported. The nanoparticles convert the epoxy surface to a superhydrophobic one with a high static contact angle (~151º-155º) and low contact angle hysteresis (~1º-3.7º) where droplets of water are in the suspended Cassie-Baxter state. The frequency decrease of the fully immersed QCM with the superhydrophobic surface is less than with only epoxy layer, thus indicating a decoupling of the QCM response. A wettability transition to a liquid penetrating into the surface roughness state (for droplets a high contact angle hysteresis Wenzel state) was triggered using a molarity of ethanol droplet test (MED) and electrowetting; the MED approach caused some surface damage. The electrowetting induced transition caused a frequency decrease of 739 Hz at a critical voltage of ~100 V compared to the QCM in air. This critical voltage correlates to a contact angle decrease of 26º and a high contact angle hysteresis state in droplet experiments. These experiments provide a proof-of-concept that QCMs can be used to sense wetting state transitions and not only mass attachments or changes in viscosity-density products of liquids
Hydrogen production through water splitting at low temperature over Fe3O4 pellet: Effects of electric power, magnetic field, and temperature
No full textThis paper aims to propose an innovative breakthrough methodology for hydrogen production through water splitting over Fe3O4 pellet at low temperature (T = 250 °C; 290 °C; 310 °C). In order to achieve this goal, the effects of magnetic field (B = 0 mT; 25.4 mT; 35.1 mT; 48.3 mT) and of electric power (P = 5 W; 12 W; 20 W) on reactive medium performance were investigated. Results show that production of hydrogen was mainly influenced by electric power applied and magnetic field, since the higher the magnetic field and the electric power the higher the production of hydrogen, while the temperature showed a secondary effect; however, feasible production of hydrogen was achieved at a temperature close to 300 °C
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Andrew Curran : Sublime disorder. Physical monstrosity in Diderot's universe. (SVEC 2001 : 01)
No full textBarroux Gilles. Andrew Curran : Sublime disorder. Physical monstrosity in Diderot's universe. (SVEC 2001 : 01). In: Dix-huitième Siècle, n°34, 2002. Christianisme et Lumières, sous la direction de Sylviane Albertan-Coppola et Antony McKenna. p. 668
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Shear stress diminishes ACE expression and signaling in Human Safenous Vein Endothelial Cells (SVEC).
No full text<p>(A) ACE protein expression downregulation, (B) ACE phosphorylation on Ser<sup>1270</sup>, and (C) p-JNK in response to laminar shear stress. SVEC were exposed to 18 h of laminar shear stress (15 dyne/cm<sup>2</sup>; SS 18 h). Each bar represents mean ± SEM of 5 separate experiments. *p<0.05 vs static control (CTRL).</p
Analytical Model of Enhanced H2 Production from Water Vapor in Bulk Iron Oxide Pellets Using Lorentz Forces
No full textThe analytical model of the effect of Lorentz forces on enhancing, by 2 orders of magnitude, hydrogen production from water molecules in bulk iron oxide pellets is studied in this paper. This model is verified by experimental data. Dropping water molecules on the surface of an iron oxide pellet results in releasing oxygen ions, due to the existing dangling bonds at relatively elevated temperatures. These ions can be directed from the surface inward, toward the bulk of the iron oxide, due to Lorentz forces perpendicular to the surface of the magnetic pellet, thus permitting new oxygen ions to be released from water molecules. These Lorentz forces are generated by the interaction between an in-plane magnetic induction of proper orientation and an in-plane applied electric field. The oxygen ion mobilization is enabled due to the substantial amount of oxygen vacancies in the bulk of the iron oxide and is being analytically modeled as a drift-diffusion mechanism, dominated by a Lorentz force-driven drift. This process remains active as long as the paths of oxygen ion mobilization toward the inner part of the iron oxide pellet are active. The same effect should also occur in other nonstoichiometric metal oxides, provided that proper Lorentz forces are applied on the corresponding oxygen ion carriers
- …
