1,721,011 research outputs found
A fast impedance-based antimicrobial susceptibility test
No full textThere is an urgent need to develop simple and fast antimicrobial susceptibility tests (ASTs) that allow informed prescribing of antibiotics. Here, we describe a label-free AST that can deliver results within an hour, using an actively dividing culture as starting material. The bacteria are incubated in the presence of an antibiotic for 30 min, and then approximately 10
5 cells are analysed one-by-one with microfluidic impedance cytometry for 2–3 min. The measured electrical characteristics reflect the phenotypic response of the bacteria to the mode of action of a particular antibiotic, in a 30-minute incubation window. The results are consistent with those obtained by classical broth microdilution assays for a range of antibiotics and bacterial species.
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Sample pre-concentration on a digital microfluidic platform for rapid AMR detection in urine
No full textThere is a growing need for rapid diagnostic methods to support stewardship of antibiotics. We describe an analytical platform for sample concentration to detect antimicrobial resistance (AMR) genes directly from human urine for the diagnosis of Urinary Tract Infections (UTIs) that are resistant to antibiotics. A sample-processing unit concentrates plasmid DNA directly from urine using magnetic beads, followed by isothermal amplification of target genes. The sample pre-concentration unit interfaces with a digital microfluidic platform (DMF) and scales the sample volume by 500-fold, pre-concentrating DNA from 1mL into a 2L droplet for downstream processing. Tests with a clinical strain of Klebsiella pneumoniae (NCTC 13443), spiked into human urine demonstrated a limit of detection of 104 cfu/mL and a “sample to answer” in approximately 30 minutes
Dataset for Sample pre-concentration on a digital microfluidic platform for rapid AMR detection in urine
No full textAssigned DOI: 10.5258/SOTON/D0731
Data set for figures in:
Kalsi, S. et al (2018). Sample pre-concentration on a digital microfluidic platform for rapid AMR detection in urine. Lab on a Chip., DOI: https://doi.org/10.1039/C8LC01249K</span
Dataset for: A fast impedance-based antimicrobial susceptibility test
No full textDataset for A fast impedance-based antimicrobial susceptibility test, Nature Communications, Daniel C. Spencer, Teagan Paton, Timothy J.J. Inglis, J. Mark Sutton and Hywel Morgan
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dataset for Ultra-fast electronic detection of antimicrobial resistance genes using isothermal amplification and thin film transistor sensors
No full textDataset supports:
Hu, C. et al (2017). Ultra-fast electronic detection of antimicrobial resistance genes using isothermal amplification and thin film transistor sensors. Biosensors and Bioelectronics, 96, 281-287. </span
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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