219 research outputs found
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Soltaanɪṇ zindiiṇa kisa
No full textIn this monologue Sultan Khan (a 54-year old male driver) recollects his childhood memories when he was studying in class 8th. He narrates how he once fled from home to Sargodha and then to Lahore with a friend, and about the subsequent events. The other speakers in this recording are Muhammad Rafiq (a 28-year old male) and Muhammad Mushtaq (a 25-year old male)
Recommended from our members
Soltaanɪṇ zindiiṇa kisa
No full textIn this monologue Sultan Khan (a 54-year old male driver) recollects his childhood memories when he was studying in class 8th. He narrates how he once fled from home to Sargodha and then to Lahore with a friend, and about the subsequent events. The other speakers in this recording are Muhammad Rafiq (a 28-year old male) and Muhammad Mushtaq (a 25-year old male)
Myeloperoxidase and elastase are only expressed by neutrophils in normal and in inflammed liver
No full textMyeloperoxidase (MPO) is involved in acute and chronic inflammatory diseases. The source of MPO in acute liver diseases is still a matter of debate. Therefore, we analysed MPO-gene expression on sections from normal and acutely damaged [carbon tetrachloride-(CCl4) or whole liver gamma-Irradiation] rat liver by immunohistochemistry, real time PCR and Western blot analysis of total RNA and protein. Also total RNA and protein from isolated Kupffer cells, hepatic stellate cells, Hepatocytes, endothelial cells and neutrophil granulocytes (NG) was analysed by real time PCR and Western blot, respectively. Sections of acutely injured human liver were prepared for MPO and CD68 immunofluorescence double staining. In normal rat liver MPO was detected immunohistochemically and by immunofluorescence double staining only in single NG. No MPO was detected in isolated parenchymal and non-parenchymal cell populations of the normal rat liver. In acutely damaged rat liver mRNA of MPO increased 2.8-fold at 24 h after administration of CCl4 and 3.3-fold at 3 h after gamma-Irradiation and MPO was detected by immunofluorescence double staining only in elastase (NE) positive NGs but not in macrophages (ED1 or CD68 positive cells). Our results demonstrate that, increased expression of MPO in damaged rat and human liver is due to recruited elastase positive NGs
Radiation-induced damage of the rat intestine after external beam irradiation of the liver
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Ferritin L and ferritin H are differentially located within hepatic and extra hepatic organs under physiological and acute phase conditions
No full textFerritin L (FTL) and Ferritin H (FTH) subunits are responsible for intercellular iron storage. We previously reported increasing amounts of liver cytoplasmic and nuclear iron content during acute phase response (APR). Aim of the present study is to demonstrate intracellular localization of ferritin subunits in liver compared with extra hepatic organs of rat under physiological and acute phase conditions. Rats were administered turpentine-oil (TO) intramuscularly to induce a sterile abscess (acute-phase-model) and sacrificed at different time points. Immunohistochemistry was performed utilizing horse-reddish-peroxidise conjugated secondary antibody on 4 mu m thick section. Liver cytoplasmic and nuclear protein were used for Western blot analysis. By means of immunohistology, FTL was detected in cytoplasm while a strong nuclear positivity for FTH was evident in the liver. Similarly, in heart, spleen and brain FTL was detected mainly in the cytoplasm while FTH demonstrated intense nuclear and a weak cytoplasmic expression. Western blot analysis of cytoplasmic and nuclear fractions from liver, heart, spleen and brain further confirmed mainly cytoplasmic expression of FTL in contrast to the nuclear and cytoplasmic expression of FTH. The data presented demonstrate the differential localization of FTL and FTH within hepatic and extra hepatic organs being FTL predominantly in the cytoplasm while FTH predominantly in nucleus
Radiation-induced damage of the rat intestine after external beam irradiation of the liver
No full textFerritin L and ferritin H are differentially located within hepatic and extra hepatic organs under physiological and acute phase conditions
No full textFerritin L (FTL) and Ferritin H (FTH) subunits are responsible for intercellular iron storage. We previously reported increasing amounts of liver cytoplasmic and nuclear iron content during acute phase response (APR). Aim of the present study is to demonstrate intracellular localization of ferritin subunits in liver compared with extra hepatic organs of rat under physiological and acute phase conditions. Rats were administered turpentine-oil (TO) intramuscularly to induce a sterile abscess (acute-phase-model) and sacrificed at different time points. Immunohistochemistry was performed utilizing horse-reddish-peroxidise conjugated secondary antibody on 4 mu m thick section. Liver cytoplasmic and nuclear protein were used for Western blot analysis. By means of immunohistology, FTL was detected in cytoplasm while a strong nuclear positivity for FTH was evident in the liver. Similarly, in heart, spleen and brain FTL was detected mainly in the cytoplasm while FTH demonstrated intense nuclear and a weak cytoplasmic expression. Western blot analysis of cytoplasmic and nuclear fractions from liver, heart, spleen and brain further confirmed mainly cytoplasmic expression of FTL in contrast to the nuclear and cytoplasmic expression of FTH. The data presented demonstrate the differential localization of FTL and FTH within hepatic and extra hepatic organs being FTL predominantly in the cytoplasm while FTH predominantly in nucleus
Documenting the Burushaski language: Issues in data collection, transmission, preservation, and revitalization
No full textAs globalization increases so does the loss of world languages. Two most common reasons for language endangerment are: (1) there are few living native speakers, and (2) many children do not learn the language of their parents because other languages are considered more helpful or powerful. While the first scenario leads to rapid extinction, the second scenario, quite common in South Asia and the developing world, leads to slow language attrition and eventual death. The two types of endangered languages are under pressure to differing degrees and in different ways in relation to language maintenance. Many linguists across the world are seeking to document and preserve the so-called “endangered” languages. Taking advantage of the latest state-of-the-art technologies, documentary linguists are making efforts towards documenting and recording oral and written linguistic traditions, translating and annotating documentation materials, compiling grammatical descriptions and dictionaries, and preparing pedagogical materials for language teaching/learning for the ethnic population which claims ownership of the language. The aim of this paper is to discuss the various steps, methods, and challenges in the documentation and revitalization of Burushaski – a linguistic isolate spoken by about 90,000 people in Gilgit, Pakistan (Ethnologue 2005), and (by approximately 300 speakers) in Srinagar, India (first author 2006). Burushaski is primarily orally-preserved and literacy in the native language is practically zero. Its survival is greatly threatened by multilingualism and language shift. Documentation efforts have been conducted by western scholars, but because their publications are mostly in foreign languages (Berger 1974-1998, Tiffou 1999), they are inaccessible to Burushos who are (mainly) literate in Urdu – the prestigious language. Recently, some Pakistani scholars have produced pedagogical materials for the promotion of Burushaski but, because of limited resources and lack of training, such efforts are yet to produce substantial results. Awaiting official standardization, local rivalries have also resulted in a number of competing writing systems. Burushaski is spoken in a socio-culturally conservative and a politically volatile region and documentation efforts by non-natives encounter many obstacles. Presenting a progress report of an ongoing project (starting 2003), this paper examines the various issues in documenting Burushaski, such as: initial encounters in encouraging community participation; social, cultural, political, and financial obstacles in data collection; effective use of information technologies without disrupting community traditions; and different challenges in revitalization efforts. To meet the various project goals, we propose steps in developing an integrated framework sensitive to the issues unique to this particular situation
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