1,411 research outputs found
Effect of pre-<i>in vitro</i> maturation with cAMP modulators on the acquisition of oocyte developmental competence in cattle
No full textThe administration of follicle-stimulating hormone (FSH) prior to oocyte retrieval improves oocyte developmental competence. During bovine embryo production in vitro, however, oocytes are typically derived from FSH-unprimed animals. In the current study, we examined the effect of pre-in vitro maturation (IVM) with cAMP modulators, also known as the second messengers of FSH, on the developmental competence of oocytes derived from small antral follicles (2–4 mm) of FSH-unprimed animals. Pre-IVM with N6,2ʹ-O-dibutyryladenosine 3′,5′-cyclicmonophosphate (dbcAMP) and 3-isobutyl-1-methylxanthine (IBMX) for 2 h improved the blastocyst formation in oocytes stimulated by FSH or amphiregulin (AREG). Furthermore, pre-IVM enhanced the expression of the FSH-or AREG-stimulated extracellular matrix-related genes HAS2, TNFAIP6, and PTGS2, and epidermal growth factor (EGF)-like peptide-related genes AREG and EREG. Additionally, pre-IVM with dbcAMP and IBMX enhanced the expression of EGFR, and also increased and prolonged cumulus cell-oocyte gap junctional communication. The improved oocyte development observed using the pre-IVM protocol was ablated by an EGF receptor phosphorylation inhibitor. These results indicate that pre-IVM with cAMP modulators could contribute to the acquisition of developmental competence by bovine oocytes from small antral follicles through the modulation of EGF receptor signaling and oocyte-cumulus/cumulus-cumulus gap junctional communication. © 2018 by the Society for Reproduction and Development
DEVELOPMENT OF PRE-IMPLANTATION EMBRYO ASSESSMENT TECHNIQUES TO CREATE OPTIMAL CULTURE ENVIRONMENTS
No full text近畿大学Kindai University博士（工学）主査：山縣 一夫 教授
学内授与番号：生第66号Tatsuma Yao, Rie Suzuki, Natsuki Furuta, Yuka Suzuki, Kyoko Kabe, Mikiko Tokoro, Atsushi Sugawara, Akira Yajima, Tomohiro Nagasawa, Satoko Matoba, Kazuo Yamagata, Satoshi Sugimura
"Live-cell imaging of nuclear–chromosomal dynamics in bovine in vitro fertilised embryos. " Scientific Reports 2018, 8 (1), 7460
Tatsuma Yao, Akane Ueda, Atchalalt Khurchabilig, Daisuke Mashiko, Mikiko Tokoro, Hiroki Nagai, Tei Sho, Satoko Matoba, Kazuo Yamagata, Satoshi Sugimura "Micronucleus formation during early cleavage division is a potential hallmark of preimplantation embryonic loss in cattle." Biochemical and Biophysical Research Communications, 617(2022), Pages 25-32
https://doi.org/10.1016/j.bbrc.2022.05.075 掲載doctoral thesi
Promotion of EGF receptor signaling improves the quality of low developmental competence oocytes
No full textAvailable online 14 May 2015Abstract not availableSatoshi Sugimura, Lesley J. Ritter, Ryan D. Rose, Jeremy G. Thompson, Johan Smitz, David G. Mottershead, Robert B. Gilchris
A successful model of regional healthcare information exchange in Japan: Case Study in Kagawa Prefecture
No full textIn this study, we focused on analysis of healthcare data exchange over the network. For the advance of broadband capability development, many governments expect online medical information exchange between medical institutions. Japanese government also has tried to deploy ICT in the healthcare field. In Japan, many healthcare ICT projects started, but almost of all the projects face many issues and failed to continue. This situation caused us to clarify the success factor of healthcare information exchange network. For inspecting the success factors, we analyzed information access of healthcare systems in Kagawa prefecture of Japan. Kagawa prefecture is one of the most advance areas for healthcare information technology. We analyzed four medical ICT projects in Kagawa prefecture: K-MIX, Critical Pathway for Diabetes, E-prescription, and PHR. In addition, we inspected characteristics of exchanged data in the network, and stakeholder involved in these projects. This analysis lets us find various types of healthcare ICT projects. Characteristic of data processed in the projects caused differences of characteristic of the projects. On the other hand, multiple systems process same data, though the project does not share the data itself. Considering various types of medical information exchanges projects, we propose classification and standard format of exchanged data according to their characteristic are critical for efficient business deployment. --e-Health,regional healthcare information exchange,EHR
Analysis for science librarians of the 2015 Nobel Prize in Physiology or Medicine: the life and work of William C. Campbell, Satoshi Ōmura, and Youyou Tu
No full textParasites affect humans worldwide with varying degrees of seriousness. Some of the most impactful parasitic infections affect millions of people, many of whom are already impoverished and struggling. The discoveries of the 2015 Nobel Laureates in Physiology of Medicine have changed the way some of these serious parasitic infections are treated, saving and improving the lives of countless people. These Laureates are William C. Campbell, Satoshi Ōmura, and Youyou Tu
Oocyte induction of EGF responsiveness in somatic cells is associated with the acquisition of porcine oocyte developmental competence
No full textOocytes progressively acquire the competence to support embryo development as oogenesis proceeds with ovarian folliculogenesis. The objectives of this study were to investigate oocyte-secreted factor (OSF) participation in the development of somatic cell epidermal growth factor (EGF) responsiveness associated with oocyte developmental competence. A well-established porcine model was employed using oocytes from small (4 mm) antral follicles, representing low vs moderate developmental competence, respectively. Cumulus-oocyte complexes (COCs) were treated in vitro with inducers of oocyte maturation, and cumulus cell functions and oocyte developmental competence were assessed. COCs from small follicles responded to FSH but, unlike COCs from larger follicles, were incapable of responding to EGF family growth factors known to mediate oocyte maturation in vivo, exhibiting perturbed cumulus expansion and expression of associated transcripts (HAS2 and TNFAIP6). Low and moderate competence COCs expressed equivalent levels of EGF receptor (EGFR) mRNA; however, the former had less total EGFR protein leading to failed activation of phospho-EGFR and phospho-ERK1/2, despite equivalent total ERK1/2 protein levels. Native OSFs from moderate, but not from low, competence oocytes established EGF responsiveness in low competence COCs. Four candidate recombinant OSFs failed to mimic the actions of native OSFs in regulating cumulus expansion. Treatment with OSFs and EGF enhanced oocyte competence but only of the low competence COCs. These data suggest that developmental acquisition by the oocyte of capacity to regulate EGF responsiveness in the oocyte's somatic cells is a major milestone in the oocyte's developmental program and contributes to coordinated oocyte and somatic cell development.Lesley J. Ritter, Satoshi Sugimura, and Robert B. Gilchris
Oocyte maturation and quality: role of cyclic nucleotides
No full textAdvance Publication first posted online on 15 July 2016 - Accepted manuscriptThe cyclic nucleotides, cAMP and cGMP, are the key molecules controlling mammalian oocyte meiosis. Their roles in oocyte biology have been at the forefront of oocyte research for decades and many of the long standing controversies in relation to the regulation of oocyte meiotic maturation are now resolved. It is now clear that the follicle prevents meiotic resumption through the actions of natriuretic peptides and cGMP inhibiting the hydrolysis of intra-oocyte cAMP and that the preovulatory gonadotrophin surge reverses these processes. The gonadotrophin surge also leads to a transient spike in cAMP in the somatic compartment of the follicle; research over the past 2 decades has conclusively demonstrated that this surge in cAMP is important for the subsequent developmental capacity of the oocyte. This is important, as oocyte in vitro maturation (IVM) systems practiced clinically do not recapitulate this cAMP surge in vitro, possibly accounting for the lower efficiency of IVM compared to clinical IVF. This review focuses in particular on this latter aspect - the role of cAMP/cGMP in the regulation of oocyte quality. We conclude that clinical practice of IVM should reflect this new understanding of the role of cyclic nucleotides, thereby creating a new generation of ART and fertility treatment options.Gilchrist RB, Luciano AM, Richani D, Zeng HT, Wang X, De Vos M, Sugimura S, Smitz J, Richard FJ and Thompson J
Adelencyrtus odonaspidis Fullaway 1913
No full textAdelencyrtus odonaspidis Fullaway, 1913 Comments. The Japanese record is based on BURKS (1958), but this author did not state which material was examined or where the information originated.Published as part of Japoshvili, George, Higashiura, Yoshimitsu & Kamitani, Satoshi, 2016, A review of Japanese Encyrtidae (Hymenoptera), with descriptions of new species, new records and comments on the types described by Japanese authors, pp. 345-401 in Acta Entomologica Musei Nationalis Pragae 56 (1) on page 395, DOI: 10.5281/zenodo.530683
APHERP symposium session III : Higher education graduate employment for uncertain futures
No full textPresented Titles: What Can We Learn from the Past Crisis and Uncertainty? Re-examining the Impact of Global Financial Crisis on Graduate Employment in Japan [Author: Satoshi Araki] A Critical Review of Skills that Higher Education should Prepare Students for the Era of Fourth Industrial Revolution [Authors: Weiyan Xiong; Huiyuan Ye] Evaluating Intention to Migrate to the Greater Bay Cities in Mainland China: A Multi-Group Analysis among Hong Kong Adults [Authors: Joshua Ka-ho Mok; Alex Zhu; Genghua Huang] Unpacking Rising Degree Requirements in the British Labour Market [Authors: Golo Henseke; Alan Felstead; Duncan Gallie; Francis Green
Neuroendocrine differentiation in adenocarcinoma of prostate during combined androgen blockade therapy : a case report
No full text85歳男性.患者は3年半前に排尿困難で受診し, 前立腺生検でpoorly differentiated adenocarcinoma, T3bN0M0と診断された.Combined androgen blockade(CAB)療法で軽快したが, 今回, 再び排尿困難で受診となり, 直腸診で前立腺腫大を認めたため入院となった.検査では貧血と軽度の腎機能障害を認め, 腫瘍マーカーのPSAは感度以下で, CEAとCA19-9は正常値, NSEとPro-GRPが著明に上昇していた.またMRI T2強調像で内部不均一な前立腺, 精嚢の著明な腫大を認め, 更に直腸との境界は不明であったことから浸潤が疑われた.そして前回と全く異なり前立腺生検ではN/C比の高い, 高度異型核を有する未分化な細胞が充実性に存在し, 免疫染色ではPSA染色陰性, NSE, クロモグラニンA, グリメリウス染色陽性と逆の結果を示していた.NE(Neuroendocrine) carcinoma, T4N0M0と診断し, 無治療にて現在も生存中であるProstatic neuroendocrine (NE) carcinoma is a rare disease with a poor prognosis because of its rapid progression and the androgen-independent characteristic, for which no successful therapy is available presently. We report a case of NE differentiated prostate cancer, which was diagnosed as adenocarcinoma initially and progressed with NE differentiation during the combined androgen blockade therapy
- …
