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Quantitative relationships between (R<sub>1429</sub>−R<sub>416</sub>−R<sub>1865</sub>)/(R<sub>1429</sub>+R<sub>416</sub>+R<sub>1865</sub>) between LEWT in wheat (A); the 1∶1 relationship between the predicted and observed LEWT on (R<sub>1429</sub>−R<sub>416</sub>−R<sub>1865</sub>)/(R<sub>1429</sub>+R<sub>416</sub>+R<sub>1865</sub>) (B).
No full text<p>Quantitative relationships between (R<sub>1429</sub>−R<sub>416</sub>−R<sub>1865</sub>)/(R<sub>1429</sub>+R<sub>416</sub>+R<sub>1865</sub>) between LEWT in wheat (A); the 1∶1 relationship between the predicted and observed LEWT on (R<sub>1429</sub>−R<sub>416</sub>−R<sub>1865</sub>)/(R<sub>1429</sub>+R<sub>416</sub>+R<sub>1865</sub>) (B).</p
D<sub>5</sub>R and CCK<sub>B</sub>R co-regulation in HEK293-D<sub>5</sub>R-CCK<sub>B</sub>R cells.
No full text<p>(A and B) Effects of gastrin (Gas, 10<sup>−8</sup> mol/L, 3 hours) and CCK<sub>B</sub>R antagonist YF476 (YF, 10<sup>−8</sup> mol/L, 3 hours) on D<sub>5</sub>R protein and mRNA expressions in HEK293-D<sub>5</sub>R-CCK<sub>B</sub>R cells (n = 6 for protein, n = 5 for mRNA, *P<0.05 versus others, one-way factorial ANOVA, Duncan’s test). (C and D) Effects of D<sub>1</sub>R and D<sub>5</sub>R agonist fenoldopam (Fen, 10<sup>−6</sup> mol/L, 3 hours) and D<sub>1</sub>R and D<sub>5</sub>R antagonist Sch23390 (Sch, 10<sup>−6</sup> mol/L, 3 hours) on CCK<sub>B</sub>R protein and mRNA expressions in HEK293-D<sub>5</sub>R-CCK<sub>B</sub>R cells (n = 6 for protein, n = 5 for mRNA, *P<0.05 versus others, one-way factorial ANOVA, Duncan’s test). Immunoblotting results are expressed as relative density units (DU). Immunoblots of D<sub>5</sub>R, CCK<sub>B</sub>R and GAPDH are shown in the inset. mRNA expression was determined by qRT-PCR and corrected for the expression of GAPDH mRNA.</p
Allosteric interaction between A<sub>1</sub>R and A<sub>2A</sub>R in A<sub>1</sub>R-A<sub>2A</sub>R CHO cells.
No full text<p>Competition experiments were performed in membrane preparations from CHO cells expressing A<sub>1</sub>R or A<sub>1</sub>R and A<sub>2A</sub>R with 12 nM [<sup>3</sup>H]R-PIA <i>versus</i> increasing concentrations of the A<sub>2A</sub>R agonist CGS-21680 as indicated in Methods. Data represent means ± S.E.M. of a representative experiment performed with triplicates.</p
D<sub>5</sub>R and CCK<sub>B</sub>R interaction in mouse kidney.
No full text<p>(A) CCK<sub>B</sub>R protein expression in PMFs is increased in D<sub>5</sub>R gene knockout mice (D<sub>5</sub>R<sup>-/-</sup>), relative to wild-type littermates (D<sub>5</sub>R<sup>+/+</sup>). (B) D<sub>5</sub>R protein expression in PMFs is increased in CCK<sub>B</sub>R gene knockout mice (CCK<sub>B</sub>R<sup>-/-</sup>) relative to wild-type littermates (CCK<sub>B</sub>R<sup>+/+</sup>). (C) Effect of the D<sub>1</sub>R and D<sub>5</sub>R agonist fenoldopam (Fen, 1mg/kg/day, one week) on the renal membrane protein expression of CCK<sub>B</sub>R in BALB/c mice fed high salt (HS) diet. NS = normal salt. (D) Effect of gastrin (Gas, 10g/kg/day, one week) on the renal membrane protein expression of D<sub>5</sub>R in BALB/c mice on high salt (HS) diet. NS = normal salt. *P<0.05, n = 3–5 per group, Student’s t test. All immunoblotting results are expressed as relative density units (DU). Sample loading amount was quantified by bicinchoninic acid assay. Immunoblots of D<sub>5</sub>R and CCK<sub>B</sub>R are shown in the inset.</p
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Binding of the A<sub>2A</sub>R antagonists KW-6002 and SCH-442416 to A<sub>1</sub>R-A<sub>2A</sub>R and A<sub>2A</sub>R-D<sub>2</sub>R CHO cells.
No full text<p>Competition experiments of [<sup>3</sup>H]ZM-241385 (2 nM) <i>versus</i> increasing concentrations of KW-6002 (a and c) or SCH-442416 (b and d) were performed as indicated in Methods in membrane preparations from CHO cells expressing A<sub>1</sub>R and A<sub>2A</sub>R (a and b) or A<sub>2A</sub>R and D<sub>2</sub>R (c and d). Data are means ± S.E.M. of a representative experiment performed with triplicates.</p
Pharmacological parameters for SCH-442416 binding to A<sub>2A</sub>R, A<sub>1</sub>R-A<sub>2A</sub>R and A<sub>2A</sub>R-D<sub>2</sub>R CHO cells.
No full text<p>Competition experiments of [<sup>3</sup>H]ZM-241385 (2 nM) binding <i>versus</i> increasing concentrations of SCH-442416 were performed as indicated in Methods in membrane preparations from CHO cells expressing A<sub>2A</sub>R or A<sub>1</sub>R and A<sub>2A</sub>R or A<sub>2A</sub>R and D<sub>2</sub>R. Results were fitted assuming that receptors (also when heteromerizing) form homodimers, and cooperativity (D<sub>CB</sub> ≠ 0, fitting to eq. 2; <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016088#s2" target="_blank">Materials and Methods</a>) or non-cooperativity (D<sub>CB</sub> = 0, fitting to eq. 3; <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016088#s2" target="_blank">Materials and Methods</a>) of SCH-442416 binding was statistically tested (F test). K<sub>DB1</sub> and K<sub>DB2</sub> are, respectively, the equilibrium dissociation constants of the first and second binding of B (SCH-442416) to the dimer. D<sub>CB</sub> is the “dimer cooperativity” index for the binding of the ligand B, and B<sub>50</sub> is the concentration providing half saturation for B. Data are mean ± S.E.M. values of three experiments.</p><p>**: p<0.01, respectively compared to the K<sub>DB2</sub> and B<sub>50</sub> values in A<sub>2</sub>R and A<sub>1</sub>R-A<sub>2A</sub>R cells; Kruskal-Wallis, followed by Dunn's test.</p
D<sub>5</sub>R and CCK<sub>B</sub>R physical interaction in HK-2 and HEK293-D<sub>5</sub>R-CCK<sub>B</sub>R cells.
No full text<p>Co-immunoprecipitation of D<sub>5</sub>R and CCK<sub>B</sub>R in HK-2 cells (A) and HEK293-D<sub>5</sub>R-CCK<sub>B</sub>R cells (B). Whole cell lysates were subjected to immunoprecipitation (IP) with mouse anti-D<sub>5</sub>R antibody, mouse anti-CCK<sub>B</sub>R antibody, or non-immune mouse serum (negative control). Immunoprecipitated complexes were analyzed by immunoblotting (western blot, WB), using rabbit anti-D<sub>5</sub>R antibody or rabbit anti-CCK<sub>B</sub>R antibody. PC: positive control; NC: negative control. These experiments were repeated three times with similar results.</p
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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