5,626 research outputs found
Surface expression of major peanut alergen Ara h 2 mimotopes in lactic acid bacteria Lactococcus lactis
No full textAlergije so motnje v delovanju imunskega sistema, s katerimi se dandanes spopada vse več ljudi. Obstajajo sicer različni načini zdravljenja teh bolezni, a so pogosto zahtevni, imajo resne neželene učinke, so neprijetni za pacienta in niso nujno uspešni. Vse to so razlogi, zaradi katerih se raziskovalci usmerjajo v iskanje novih možnih terapij. Velik potencial izkazujejo kratki peptidi-mimotopi, ki lahko posnemajo dejanske alergene in se vežejo na protitelesa IgE pri bolniku, a ne povzročijo alergijske reakcije ter tako sčasoma vodijo v umiritev alergijskega odziva. Odločili smo se jih raziskati v tej magistrski nalogi. Izbrali smo mimotope, ki posnemajo glavni alergen arašidov, saj je prav alergija za arašide ena najpogostejših. Za nosilce smo uporabili mlečnokislinske bakterije – sev Lactococcus lactis, ki veljajo za koristne in so dobro raziskane. Cilj magistrske naloge je bil pripraviti transformirane mlečnokislinske bakterije s sposobnostjo površinskega izražanja mimotopov, ki posnemajo alergen arašidov in se vežejo na protitelesa IgE. Bakterije Lactococcus lactis smo transformirali s plazmidi, ki so vsebovali zapise za izbrane peptide-mimotope, s postopkom elektroporacije. Predhodno smo njihove zapise pomnožili v bakterijah Escherichia coli. Uspešnost transformacije smo preverjali s potrjevanjem pravilnega nukleotidnega zaporedja za izbrane peptide v bakterijah, uspešnost izražanja in funkcionalnost peptidov pa s pomočjo encimsko imunoadsorpcijskega testa (ELISA) ter z metodo prenosa po westernu. S celotnim eksperimentalnim delom smo dosegli postavljeni cilj – proces transformacije je bil uspešen, saj so bakterije na svoji površini izražale želene peptide. To smo dokazali s potrjenimi ustreznimi sekvencami vstavljenega genskega zapisa. S prenosom po westernu smo dokazali prisotnost peptidov na površini L. lactis. Ko smo uporabili humani serum pacientov z alergijo, pa smo s prenosom po westernu potrdili, da so peptidi res mimotopi. Mimotopi imajo velik potencial in upamo, da bodo nadaljnje raziskave prinesle nova, boljša zdravila za alergijske bolezni.Allergies represent a dysfunction in the activity of the immune system and are affecting increasingly more people nowadays. There are different ways of treating these diseases, but these ways are often demanding, have serious side effects, are unpleasant for the patient and not necessarily successful. Due to these reasons, researchers are trying to find new therapy choices. Short chain peptides show a great potential – these are mimotopes, which can mimic allergens and bind with the antibodies IgE of a patient, but do not cause an allergic reaction and in time can appease an allergic reaction. For this master thesis we have chosen mimotopes that mimic the main allergen of peanuts, since peanut allergy is one of the most common allergies. We have used lactic acid bacteria as carriers – strain Lactococcus lactis, which are considered as useful bacteria and are extensively researched. The aim of this master thesis was to prepare transformed lactic acid bacteria that have the ability of surface expression of mimotopes that mimic the allergen of peanuts and bind with the antibodies IgE. Bacteria Lactococcus lactis have been transformed with plasmids, which carried the genes for the chosen peptides – mimotopes - by the process of electroporation. Prior to that process, we have replicated their genes in bacteria Escherichia coli. The success of the transformation was checked/tested with the affirmation of the right sequence of nucleotides for the chosen peptides in the bacteria, while the success of the expression and functionality of peptides was tested with the enzyme-linked immunosorbent assay (ELISA) and the western blot. In the experimental part, we have achieved the set goal – the process of transformation was successful, since the bacteria expressed the peptides on their surface. That was proven with the right sequences of the entered genetic code, while western blot proved the presence of peptides on the surface of L.lactis. In addition, by using the human serum of the patients with allergies and western blot we have proven the fact that peptides are indeed mimotopes. We can then conclude that peptides have a great potential and hope that further research will bring new and improved medicine for allergies
A Floating Question Mark: An Interview with Sara Hawys Roberts, Author of Withdrawn Traces: Searching For The Truth About Richey Manic
No full textAn interview with Sara Hawys Roberts, co-author of 'Withdrawn Traces: Searching For The Truth About Richey Manic' about the researching and writing of this much-anticipated book about the missing Manic Street Preacher.</p
A Floating Question Mark: An Interview with Sara Hawys Roberts, Author of Withdrawn Traces: Searching For The Truth About Richey Manic
No full textAn interview with Sara Hawys Roberts, co-author of 'Withdrawn Traces: Searching For The Truth About Richey Manic' about the researching and writing of this much-anticipated book about the missing Manic Street Preacher.</p
Sara Gossett Crigler Collection - Accession 614
No full textThe Sara Gossett Crigler Collection consists of a microfiche copy of her book titled, Education For Girls And Women In Upper South Carolina Prior to 1890 with Related Miscellaneous Articles: A Compilation by Mrs. Henry Towles Crigler (Sara Gossett Crigler), self-published in Greenville, SC on April 15, 1956. This book also includes many anecdotes and reminiscences of Sara’ family including a section devoted to the slaves owned and later freed after the Civil War by her family. The book is dedicated by the author, Sara Gossett Crigler (1886-1966), to her mother Sallie Brown Gossett (1859-1942) and her aunt Mary Brown Mahon (1861-1948) who were both graduates of Williamston Female College in 1877 and 1879 respectively. The 170 page volume would be useful to anyone doing research on the education of women in South Carolina during the 19th century. The original copy is housed at the South Carolina Historical Society as SCHS 509 and was dedicated and signed by the author, “For the Charleston Library Society” on July 10, 1964.
*Please see attached Table of Contentshttps://digitalcommons.winthrop.edu/manuscriptcollection_findingaids/1527/thumbnail.jp
Materia-autore = Author-Matter
No full textThe etymology of the word author refers to an act of creation, an act of augmentation, from the Latin verb augere. Author instantiates creation, the expansion of the pre-existing. In 1967 Roland Barthes declared the death of the author in his famous essay to state once more that the crisis is that of the author as a single subjectivity and as a term that condenses prestige, undermined by the de-subjectivation strategies of automatism, fortuity and fragmentation of the historical avant-gardes, as well as by the machinic act and by the reproducibility of the second avant-gardes.
Fifty years after Barthes’ paradigmatic formula, this lack of authorship appears to be a successful brand. The ten- sions between the anomie of matter, the law that establishes authorship and the economy that makes the work pos- sible, invoke discordant perspectives. Artists make the self-destruction of their work the real work, and appeal is made for the demolition of architectures, whether by a recognised author or not, in order to re-design, or better still, re-claim the territory. Artificial intelligence consolidates its logics and its design by progressively shedding human ingenuity. The space of criticism becomes, finally, increasingly ephemeral. However, there is an acceptation of criti- cism that is, rather than an individual ‘signature’, an exploration and explanation of how design makes theory.
The binomial author-matter seeks to mark these tensions and contradictions: the featured term author is main- tained to underline the persistence of that prestigious subjectivity, at the very moment when the rhetoric of “mat- ter as an author” promises other forms of authorship
Sara Winthrop Smith letter to Frances Casement, August 14, 1887
No full textLetter written to Frances Casement from Sara Winthrop Smith of Cincinnati, Ohio, August 14, 1887. Winthrop expresses the challenges of generating support for the suffrage movement among the conservative residents of her city, and encourages the creation of clear materials that make the argument for women's suffrage to be more widely distributed.
This item comes from the Frances Jennings Casement Papers, a manuscript collection comprised of letters and association records related to the founding and leadership of the Ohio Woman Suffrage Association. Casement (1840-1928) was born in Painesville, Ohio, and graduated from Painesville Academy and Willoughby Female Seminary. Her father, Charles Casement, supported abolition and women's suffrage and encouraged Frances to be active in social causes. Frances Casement established the Painesville Equal Rights Association in 1883, and shortly after became involved in the Ohio Woman Suffrage Association, serving as its president from 1885 to 1888
Sara Brown
No full textSara E. Brown is the Executive Director of Chhange, the Center for Holocaust, Human Rights & Genocide Education. Brown holds the first Ph.D. in comparative genocide studies from Clark University\u27s Strassler Center for Holocaust and Genocide Studies. She has presented at an array of professional conferences around the world, published pieces in academic journals, news outlets, and edited volumes, and currently serves on the Advisory Board for the International Association of Genocide Scholars.
Brown has worked and conducted research in Rwanda since 2004, served as a project coordinator in refugee camps in Tanzania, worked in refugee resettlement in Texas, and researched conflict globalization and conflict in Israel. Prior to coming to Chhange, she developed and managed post-secondary education programming for USC Shoah Foundation. She has designed and taught courses on human rights, mass violence, and history at San Diego State University, Mt San Jacinto Community College, and Worcester State University. Brown served as an academic consultant for Aegis Trust in Rwanda and as the scholarly advisor on the Genocide against the Tutsi in Rwanda for Chhange\u27s museum-quality exhibit, Journeys Beyond Genocide: The Human Experience.
She is the author of Gender and the Genocide in Rwanda: Women as Perpetrators and Rescuers and the co-editor of the forthcoming Routledge Handbook on Religion and Genocide.https://commons.erau.edu/genocide-bios/1059/thumbnail.jp
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