171,170 research outputs found
Das büßende Gretchen in der Kirche / Stich von C. W. Mieling te's Hage nach Ary Scheffer
No full textDAS BÜSSENDE GRETCHEN IN DER KIRCHE / STICH VON C. W. MIELING TE'S HAGE NACH ARY SCHEFFER
Das büßende Gretchen in der Kirche / Stich von C. W. Mieling te's Hage nach Ary Scheffer (1)
Das büßende Gretchen in der Kirche (1
Metabolite profiles of the flour of 40 wheat varieties grown at three location.
No full textA total of 18 old (year of release 1962-1999) and 22 modern (2005-2014) winter wheat
varieties were used for this study (Suppl. Fig. 1). All varieties, which will be designated in the
following as genotypes, have been cultivated in the season 2015/16 in 5m2 plots in Germany
at o=3 locations. Two locations were located in Baden-Wuerttemberg, namely Hohenheim
(HOH) and Ihinger Hof (IHO), as well as at Gatersleben (GAL) in Saxony-Anhalt. The
experimental design was an incomplete block design with one replication per location. The metabolite analyses of our study were based on the wheat flour samples collected for
the 40 wheat varieties from all three locations. Each of the 120 samples was analyzed one
time via gas chromatography-mass spectrometry (GC-MS) using an adapted protocol from
Lisec et al. (2006). Metabolites were extracted from 45-55 mg dry flour samples with 750 μl
of a 1:2.5:1 H2 O:methanol:chloroform (v:v:v) mixture pre-cooled to -20 ◦ C, then mixed on a
rotator for 10 min and centrifuged at 20,000 g for 2 min (both at 4 ◦C). A total of 50 μl of
the supernatant were dried completely in a vacuum concentrator and derivatized in two steps
via an MPS-Dual-head autosampler (Gerstel): (1) with 10 μl methoxyamine hydrochloride
(Acros organics; freshly prepared at 20 mg/ml in pure pyridine (Sigma-Aldrich)) and shaking
◦at 37 C for 90 min, (2) adding 90 μl N-Methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA;
◦Macherey-Nagel) and shaking at 37 C for 30 min. After incubation for 2 hours at room
temperature, 1 μl of derivatized compounds was injected at a flow of 1 ml/min with an
automatic liner exchange system in conjunction with a cold injection system (Gerstel) in
◦ ◦ ◦splitless mode (ramping from 50 C to 250 C at 12 C/s) into the GC. Chromatography
was performed using a 7890B GC system (Agilent Technologies) with a 30 m long, 0.25
mm internal diameter, HP-5MS column with 5% phenyl methyl siloxane film (Agilent
19091S-433). The oven temperature was held constant at 70◦ C for 2 min and then ramped at
12.5◦C/min to 320◦ C at which it was held constant for 5 min; resulting in a total run time of
27 minutes
Trials and tribulations of trials in Obstetrics, evaluation of the randomised controlled trials of C-STICH, PREPS and C-STICH2
No full textIntroduction:
An evaluation of trials in obstetrics.
PREPS - Vaginal Preparation with chlorhexidine at Caesarean Section (CS) to Reduce Endometritis and Prevent Sepsis: feasibility RCT.
C-STICH- Cerclage Suture Type for an Insufficient Cervix and its effect on Health outcomes.
C-STICH2- RCT of emergency cervical cerclage vs expectant management in women with bulging membranes.
Methods:
PREPS –there were uncertainties regarding the ability to recruit (screen, consent and randomise) women undergoing emergency CS. Process were developed to overcome these challenges and tested within a feasibility RCT.
C-STICH – an evaluation of the challenges of recruiting women undergoing a rare surgical procedure and managing a lower than anticipated control group event rate.
C-STICH2 – to access the feasibility of recruitment when prognosis poor. Accessed via an RCT with internal pilot and embedded qualitative evaluation.
Results:
PREPS demonstrated it is feasible to perform an RCT of vaginal cleansing at CS to reduce SSI, using bespoke verbal consent and telephone randomisation.
C-STICH demonstrated that by establishing a large clinical network you can recruit women undergoing a rare procedure.
C-STICH 2 demonstrated the importance of including a mixed methods evaluation in trials when recruitment is challenging.
Conclusion:
New processes allow consent, randomisation and follow up in difficult trials
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
The role of space, time and sociability in predicting social encounters
No full textSpace, time and the social realm are intrinsically linked. While an array of studies have tried to untangle these factors and their influence on human behaviour, hardly any have taken their effects into account at the same time. To disentangle these factors, we try to predict future encounters between students and assess how important social, spatial and temporal features are for prediction. We phrase our problem of predicting future encounters as a link-prediction problem and utilise set of Random Forest predictors for the prediction task. We use data collected by the Copenhagen network study; a study unique in scope and scale and tracks 847 students via mobile phones over the course of a whole academic year. We find that network and social features hold the highest discriminatory power for predicting future encounters
Mitomycin C in highly myopic eyes - Author reply
No full textOphthalmology. 2005 Feb;112(2):208-18; discussion 219.
Mitomycin C modulation of corneal wound healing after photorefractive keratectomy in highly myopic eyes.
Gambato C, Ghirlando A, Moretto E, Busato F, Midena E.
SourceRefractive Surgery Service and Antimetabolite Therapy Research Unit, Department of Ophthalmology, University of Padova, Padova, Italy.
Abstract
PURPOSE: To evaluate the role of topical mitomycin C in corneal wound healing (CWH) after photorefractive keratectomy (PRK) in highly myopic eyes.
DESIGN: Prospective, double-masked, randomized clinical trial.
PARTICIPANTS: Seventy-two eyes of 36 patients affected by high (>7 diopters) myopia.
METHODS: In each patient, one eye was randomly assigned to PRK with intraoperative topical 0.02% mitomycin C application, and the fellow eye was treated with a placebo. Postoperatively, mitomycin C-treated eyes received artificial tears (3 times daily, tapered in 3 months), whereas the fellow eye was treated with fluorometholone sodium 2% and artificial tears (3 times daily, tapered in 3 months).
MAIN OUTCOME MEASURES: Uncorrected visual acuity (UCVA) and best-corrected visual acuity (BCVA), contrast sensitivity, manifest refraction, and biomicroscopy. Contrast sensitivity was determined using the Pelli-Robson chart. Corneal confocal microscopy documented CWH.
RESULTS: Mean follow-up was 18 months (range, 12-36). No side effects or toxic effects were documented. At 12-month follow-up examination, UCVAs (logarithm of the minimum angle of resolution) were 0.4+/-0.48 and 0.5+/-0.53 (P = .03) in mitomycin C-treated eyes and corticosteroid-treated eyes, respectively. At 1 year, corneal haze developed in 20% of corticosteroid-treated eyes, versus 0% of mitomycin C-treated eyes. At 12, 24, and 36 months, corneal confocal microscopy showed activated keratocytes and extracellular matrix significantly more evident in untreated eyes (Ps = 0.004, 0.024, and 0.046, respectively).
CONCLUSION: Topical intraoperative application of 0.02% mitomycin C can reduce haze formation in highly myopic eyes undergoing PRK.
Comment in
Ophthalmology. 2006 Feb;113(2):357; author reply 357-8
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Display of Plasmodium adhesive domains on lambda phages.
No full textEfficient transmission of the malaria parasite by the mosquito vector requires successful invasion of the salivary glands, a process involving specific molecular interactions. Although different studies in the last decade highlighted the possible role of a few sporozoite surface proteins the key players of the invasion process have remained elusive so far. With the objective to improve our understanding of sporozoites-salivary gland interaction, we are using an approach that involves the display on the lambda surface of putative adhesive domains from a few selected Plasmodium proteins possibly implicated in salivary gland recognition/invasion. This collection of lambda phages can be employed for the development of affinity selection experiments targeted to the identification of salivary gland receptors. The lambda system allows the display of relatively large domains (up to 400 aminoacids) and, therefore, it is particularly suitable for our purpose. As a first step toward this direction, adhesive domains from selected P. falciparum and P. berghei surface proteins (such as TRAP, CS, MAEBL, etc) have been fused to the C-terminus of the abundant capsid protein gpD. The different domains were PCR-amplified, cloned in a plasmid vector, sequenced and finally ligated into the lambda vector λD4 (kindly provided by Dr. S. Nasi, CNR, Rome). After packaging, different recombinant lambda clones were randomly isolated from this phage collection and analyzed by western blot to verify the expression of the fusion proteins. All lambda clones identified and isolated so far seem to express recombinant proteins of the expected size. Verification of correct display for all selected domains, as well as binding assays to mosquito salivary glands, is in progress
Preparation and calibration of pressure-sensitive and temperature-sensitive paints for fluorescence lifetime imaging applications
No full textThis thesis describes various methods for time-resolved luminescence lifetime determination, and their applications to imaging barometric pressure and temperature. These approaches are superior to luminescence intensity imaging in that they are less influenced by sources of errors like light scattering, inhomogeneous indicator distribution, photobleaching, or background fluorescence because they are intrinsically referenced. The data obtained are precise and unambiguous. Novel sensor materials and methods for time-resolved imaging are described, characterized, and applied.
A novel dual pressure-sensitive paint (PSP) and temperature-sensitive paint (TSP) system for time resolved luminescence imaging is introduced, consisting of a platinum porpholactone and a ruthenium-diimine complex,. The dual paint was applied to an aircraft model for wind tunnel testing. The dual paint has proven to be applicable for aerodynamic research. Temperature can be determined with an accuracy of 0.5 K and the surface pressure topology coincides with theory and with the data from conventional pressure measurements.
Furthermore, a novel kind of an optical dual sensor is introduced, allowing for signal separation due to different luminescence lifetimes without the need of additional spectral separation. This technique expands the possible spectrum of combination of probes applicable to optical dual sensing. The new sensor system consists of a platinum porphyrin and a europium chelate complex, capable of determining oxygen and temperature simultaneously, without the need for different optical filters.
A novel general approach for imaging is presented. It makes use of the fundamental setup of digital cameras and enables to monitor the spectrally resolved luminescence of up to three signals with one single image.
The new materials and methods developed during this dissertation led to the fabrication of the first optical triple sensor systems. Two kinds of sensors are reported. They are capable of simultaneously determining the three important analytes pH, T, and O2. The first system consisted of HPTS as the pH-indicator, a Eu-chelate complex as the temperature probe, and a Pt-porpholactone as the probe for oxygen, all encapsulated in appropriate polymer particles. The three signals are separated using optical filters. In the second triple sensor system, the O2 probe of the first system was replaced by a Pt-porphyrin, enabling for time-resolved separation of the temperature signal from the O2 signal. With these new kinds of sensors, it is possible to monitor the three clinically relevant parameters pH, T, and O2 non-invasively, online, and in surpassing spatial resolution. By omitting the oxygen probe, the first temperature compensated optical pH sensor ever described in literature is obtained
A Multi-Language Comparison of Influences on Author Verification using Character N-Grams
No full textWe create a new multi-language corpus for author verification based on Wikipedia talkpages, and evaluate the influence that differences in topic and time have on character n-gram author profiles. Topic alignment between two texts is found to increase author verification precision, and an authors writing style is found to change over time, but not more significantly after 3 years than after 1 year.Information ArchitectureWISElectrical Engineering, Mathematics and Computer Scienc
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