1,721,552 research outputs found
SUPERSEDED: Primary data for manuscript on a homologue of lymphostatin in Chlamydia pecorum
No full text## ## This item has been replaced by the one which can be found at https://hdl.handle.net/10283/9002 ## Pathogens frequently produce proteins to evade or inhibit host immune responses. One such protein is lymphostatin from attaching and effacing Escherichia coli (also known as lymphocyte inhibitory factor A; LifA), which influences intestinal colonization and inhibits mitogen- and antigen-activated proliferation of T lymphocytes and pro-inflammatory cytokine synthesis. Here, we report the cloning, purification and characterization of a LifA homologue from Chlamydia pecorum. The predicted 382 KDa protein (CPE2_0552) exhibited 36 % identity and 55 % similarity over 3171 amino acids to lymphostatin from enteropathogenic E. coli strain E2348/69. CPE2_0552 shares glycosyltransferase and cysteine protease motifs required for lymphostatin activity, including similarity in the tertiary structure of these domains predicted by AlphaFold 3. Purified CPE2_0552 exhibited a surface envelope similar to that of lymphostatin when analyzed by electron microscopy. CPE2_0552 inhibited concanavalin A-stimulated proliferation of bovine T cells in a concentration-dependent manner, with an inhibitory dose 50 (ID50) of 990 pg/mL. This was 70-fold higher than the ID50 of E. coli E2348/69 lymphostatin tested in parallel on T cells from the same donors (14 ± 4 pg/mL), but was similar to another LifA homologue from E. coli O157:H7 (ToxB). Moreover, CPE2_0552 inhibited the secretion of interferon gamma (IFN?), a key cytokine that influences the outcome of Chlamydia infections. At the concentrations at which CPE2_0552 inhibited T lymphocyte proliferation and IFN? secretion, negligible cytotoxicity was observed after 72 h of stimulation. Our study indicates that E. coli lymphostatin belongs to a wider family of lymphocyte-inhibitory molecules that exist in distantly related bacterial pathogens
FIGURES 1‒3 in A revision of the genus Lepidobrya Womersley (Collembola: Entomobryidae) based on morphology and sequence data of the genotype
No full textFIGURES 1‒3. Colour pattern of two individuals in Lepidobrya mawsoni. Scale bars: 1 mm.Published as part of Zhang, Feng, Greenslade, Penelope & Stevens, Mark I., 2017, A revision of the genus Lepidobrya Womersley (Collembola: Entomobryidae) based on morphology and sequence data of the genotype, pp. 523-536 in Zootaxa 4221 (5) on page 525, DOI: 10.11646/zootaxa.4221.5.2, http://zenodo.org/record/25349
A taxonomic revision of the tube-web spiders of the genus Ariadna (Araneae: Segestriidae) in Tasmania
No full textMarsh, Jessica R., Stevens, Mark I., Framenau, Volker W. (2022): A taxonomic revision of the tube-web spiders of the genus Ariadna (Araneae: Segestriidae) in Tasmania. Zootaxa 5105 (2): 151-201, DOI: 10.11646/zootaxa.5105.2.
Evaluation of the CytoRich(R) technique for cervical smears
No full textStevens, Mark W. ; Nespolon, Walter W. ; Milne, Amanda J. ; Rowland, Rober
Gasteruptiinae
No full textSubfamily Gasteruptiinae Ashmead, 1900 Gasteruptioninae Ashmead, 1900: 7–8. Gasteruptiinae Schulz, 1906: 133 [emendation].Published as part of Parslow, Ben A., Stevens, Mark I. & Schwarz, Michael P., 2018, First record of Gasteruption Latreille (Hymenoptera: Evanioidea: Gasteruptiidae) from Fiji with the description of a new species, pp. 111-116 in Zootaxa 4407 (1) on page 112, DOI: 10.11646/zootaxa.4407.1.7, http://zenodo.org/record/121667
Friesea Dalla Torre 1895
No full textGenus Friesea Dalla Torre, 1895 Friesea Dalla Torre, 1895: 14. Polyacanthella Schäffer, 1897: 15. TYPE SPECIES. — Friesea mirabilis (Tullberg, 1871).Published as part of D'Haese, Cyrille A., Stevens, Mark I. & Weiner, Wanda M., 2017, Friesea najtae n. sp. (Collembola, Neanuridae, Frieseinae) from southern Western Australia, pp. 21-29 in Zoosystema 39 (1) on page 22, DOI: 10.5252/z2017n1a3, http://zenodo.org/record/457846
FIG. 2 in Friesea najtae n. sp. (Collembola, Neanuridae, Frieseinae) from southern Western Australia
No full textFIG. 2.— Live specimen of Friesea najtae n. sp photographed in its natural habitat. Photograph: Cyrille A. D'Haese.Published as part of D'Haese, Cyrille A., Stevens, Mark I. & Weiner, Wanda M., 2017, Friesea najtae n. sp. (Collembola, Neanuridae, Frieseinae) from southern Western Australia, pp. 21-29 in Zoosystema 39 (1) on page 23, DOI: 10.5252/z2017n1a3, http://zenodo.org/record/457846
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
FIGURES 4‒9 in A revision of the genus Lepidobrya Womersley (Collembola: Entomobryidae) based on morphology and sequence data of the genotype
No full textFIGURES 4‒9. Scales/chaetae in the posterior row along tergal margin in Lepidobrya mawsoni, left side except Fig. 9. 4, Th. II; 5, Th. III; 6, Abd. I; 7, Abd. II; 8, Abd. III; 9, Abd. IV. Scale bars: 4‒8, 50 µm; 9, 120 µm.Published as part of Zhang, Feng, Greenslade, Penelope & Stevens, Mark I., 2017, A revision of the genus Lepidobrya Womersley (Collembola: Entomobryidae) based on morphology and sequence data of the genotype, pp. 523-536 in Zootaxa 4221 (5) on page 526, DOI: 10.11646/zootaxa.4221.5.2, http://zenodo.org/record/25349
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