303 research outputs found
Mine Drainage
No full textStephen Michell's 1881 work covers the full range of engines and steam-pumps available for draining mines in the nineteenth century. An expert on contemporary mining technology, Michell co-authored the essays 'The Best Mining Machinery' and 'The Cornish System of Mine Drainage' prior to writing this comprehensive survey. Mine Drainage represents the first attempt to gather in one book information previously located in various journals (and therefore difficult to find), and documentation about engines by their (possibly biased) manufacturers. The book also contains almost 140 illustrations of the diverse pumps and engines discussed. After a short introduction, the material is organised into two main sections, focusing on horizontal and vertical engines. Within those categories it discusses rotary and non-rotary engines, and simple and compound steam-pumps. The book will interest historians of technology, science, engineering, and mining in the Victorian period.</jats:p
Our glorious dead [music] : memorial song to British Empire /
No full textCover title.; "This beautiful song should be sung on all memorial occasions it is dedicated to our heroes who fell in the great war".; Also available online http://nla.gov.au/nla.mus-an5528003; N, M 160818
The Critics : Rabbitt Like Pretty Automatons
No full textThe first article is a duplicate of an item forming part of hy-dm-news-reviews-michell-1977-001The article containing information about Brian Finch and Kenny Henson has been underlined in red pen.Item located in file hy-dm-finch-henson-1947-2007-001. Not all items in folder uploaded.The first article pertains to a concert featuring Rabbitt/ 5000 Volts and Brian Finch with Kenny Henson at the Film Trust Arena whereby the author reflects on all the musicians, points out the faults and provides praise where warranted. The second article refers to the same concert which took place at the Arena the previous evening. Apart from mentioning some commendable points of the evening, the author discusses the shortfalls attached to the Rabbitt performance and points out the similarities between the Henson and finch performance compared to their previous appearances
A strain tensor method for three-dimensional optimal Michell structures
No full textThesis: M. Eng., Massachusetts Institute of Technology, Department of Civil and Environmental Engineering, 2016.This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.Cataloged from student-submitted PDF version of thesis.Includes bibliographical references (pages 94-95).In the design of discrete structures such as trusses and frames, important quantitative goals such as minimal weight or minimal compliance often dominate. Many numerical techniques exist to address these needs. However, an analytical approach exists to meet similar goals, which was initiated by A.G.M. Michell (1904) and has been mostly used for two-dimensional structures so far. This thesis develops a method to extend the existing mainly two-dimensional approach to apply to three-dimensional structures. It will be referred as the Michell strain tensor method (MSTM). First, the proof that MSTM is consistent with the existing theory in two dimensions is provided. Second, two-dimensional known solutions will be replicated based on MSTM. Finally, MSTM will be used to solve new three- dimensional cases.by Benjamin Jacot.M. Eng
Cookbooks: the Sandy Michell collection: an exhibition of material from the Monash University Rare Books Collection 22 March - 31 Mat 2011
No full textThe exhibition was held in the Rare Books Exhibition space, Sir Louis Matheson Library, Monash University from 22 March - 31 May 2011 Opening address given by Rita Erlich, former editor, The Age Good Food Guide. This exhibition celebrates the gift of valuable seventeenth to nineteenth century French and English cookbooks made by Alexandra (Sandy) Michell, beginning in 1988. The collection has been further developed and expanded to include a fine collection of early Australian cookbooks, and a selection of twentieth century material
Characterisation of lipoprotieins of Clostridium difficile and their role in virulence
No full textAntibiotic-associated diarrhoea (AAD) and colitis, with the causative agent being the Gram-positive anaerobe, Clostridium difficile, are some of the most important hospital-acquired infections and significant burdens to healthcare services worldwide. Treatment of the infection is often ineffective and currently no vaccine is available against C. difficile infection (CDI). Research to identify novel virulence factors potentially leads to the development of new therapeutic and prophylactic drugs. As lipoproteins have been shown to play key roles in the virulence of several pathogens, the aim of this project was to investigate whether lipoproteins are involved in the virulence of C. difficile.
Lipoproteins are anchored to the extracellular side of the cytoplasmic membrane in Gram-positive bacteria. Two enzymes are involved in the biosynthesis of lipoproteins: lipoprotein diacylglycerol transferase (Lgt) attaches lipoproteins to the membrane, and lipoprotein signal peptidase (Lsp) cleaves the signal peptide from the amino-terminus of lipoproteins. In order to study lipoprotein processing in C. difficile, lgt and lsp mutants of the C. difficie 630Δerm strain were generated using the ClosTron system. Antibody reactivity of 14 C. difficile lipoproteins was also investigated. It was shown in this study that lgt mutation caused changes in the lipoproteome of C. difficile. Therefore, inactivation of the lgt gene allowed investigation of the global contribution of lipoproteins to bacterial processes. The physiology and virulence of the lgt mutant was studied in vitro and in vivo. Surprisingly, many of the assayed phenotypes were not significantly affected by disruption of the lgt gene. Nevertheless, the ability of the lgt mutant to adhere to Caco-2 cells was markedly reduced. In addition, the phenotype of the lgt mutant observed in mice suggests that the faecal shedding of C. difficile is affected by Lgt inactivation. In further studies, the CD0873 lipoprotein as a potential adhesin of C. difficile was identified by in silico approach. Contribution of the CD0873 lipoprotein to the adherence of C. difficle was investigated by several different assays and the results strongly suggest that the CD0873 lipoprotein is directly involved in adhesio
Investigating Pathogenesis and Virulence of the Human Pathogen, Vibrio vulnificus.
No full textV. vulnificus is a Gram negative opportunistic pathogen that is ubiquitous in the marine environment. Of the three main biotypes, biotype 1 is most commonly associated with human infection and is the causative agent of septicaemia, gastroenteritis and wound infection. In the United States V. vulnificus is the leading cause of seafood related deaths and is commonly associated with ingestion of raw or undercooked oysters. However, despite the abundant prevalence of this bacterium in the environment, the number of severe human infections is low. This has led to the hypothesis that not all strains of this pathogen are equal in virulence, with some strains better adapted to causing human disease than others.
Therefore the current study tested a panel of 10 V. vulnificus strains in several phenotypic experiments that assayed the strains for known virulence factors, with the aim of identifying a marker for strains hazardous to human health. However, not one assay correlated with either virulence potential of the strains, as determined by an in vivo mouse model of virulence, or source of isolation.
As the study hypothesised that the varying virulence potentials displayed by the strains may be due to genetic differences, whole genome sequencing (WGS) was performed. Bioinformatic comparison of the strains demonstrated many genetic differences between the strains. However, in unison with the WGS comparison, WGS gene annotation was also performed. This identified the presence of two previously undescribed type 6 secretion systems (T6SS). Therefore the current study continued investigation into the T6SSs.
The two T6SSs identified were termed T6SS1 and T6SS2. T6SS2 was found in all sequenced isolates, whereas T6SS1 was only present in a sub-set of strains. As T6SS1 shared synteny with the previously described T6SS in V. cholerae, T6SS1 was chosen for further investigation. During this study T6SS1 was shown to be functional and displayed thermoregulation. Further investigation into T6SS1 by construction and characterising of a T6SS1 mutant, demonstrated that T6SS1 contained anti-prokaryotic properties
Phenotypic Characterisation of Clostridium Difficile Strains Defective in Lipoprotein Biosynthesis
No full textClostridium difficile
is regarded as the primary etiological agent of antibiotic
-
associated diarrhoea, posing a significant challenge
to healthcare facilities. The
changing nature of
C. difficile
infection
is causing an increase in
associated
disease
occurrence
outside of the healthcare setting and a
gradual move away
from the historical
association with antimicrobial treatment. Adhesio
n of spores
and vegetative cells to host gut epithelium is thought to be a key aspect of
C.
difficile
virulence; disruption of this process may significantly reduce the impact
of an infection and the likelihood of infection spread. Lipoproteins are involve
d
in adhesion of
C. difficile
to host tissues
and may have roles in other key
aspects of virulence.
Lipoproteins
undergo a specific biosynthesis process
within the bacterial cell involving addition of an acyl
-
glyceryl moiety by
lipoprotein glyceryl transfe
rase (Lgt) followed by signal peptide cleavage by
lipoprotein signal peptidase (LspA); disruption of
this
process may cause
attenuation of virulence and
a
reduction in adhesion
to host tissue
.
C. difficile
has been shown to encode two functional and homolo
gous lipoprotein signal
peptidases: LspA and LspA2.
The novel antimicrobials globomycin and
myxovirescin directly target lipoprotein signal peptidases and therefore may
have potential for use in treatment of
C. difficile
infection.
Evaluation of their
effi
cacy against LspA and LspA2 can be determined by protection assays using
Escherichia coli
strains expressing LspA or LspA2 from
C. difficile
.
In this study, both LspA and LspA2 from
C. difficile
are shown to contain the
consensus sequences, domains and
in
silico
predicted tertiary structure
expected of lipoprotein signal peptidases. Characteris
ation of
C. difficile
strains
with silencing mutations in either
lspA
or
lspA2
,
in
comparison to a wild type
,
reveals that the absence of either lipoprotein signal pe
ptidase causes an
increased survivability in hydrogen peroxide and may affect protein
localis
ation
within the bacterium.
Finally, successful
cloning of
C. difficile lspA
and
lspA2
and subsequent expression of LspA and LspA2 via auto
-
induction in
E. coli
is
reported, paving the way for further investigation into the effect of globomycin or
myxovirescin treatment
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Technology acceptance and care self-management: consideration in context of chronic care management
No full textWith an aging global population, the number of people living with a chronic illness is expected to increase significantly by 2050. If left unmanaged, chronic care leads to serious health complications, resulting in poor patient quality of life and a costly time bomb for care providers. If effectively managed, patients with chronic care tend to live a richer and more healthy life, resulting in a less costly total care solution. This chapter considers literature from the areas of technology acceptance and care self-management, which aims to alleviate symptoms and/or reason for non-acceptance of care, and thus minimise the risk of long-term complications, which in turn reduces the chance of spiralling health expenditure. By bringing together these areas, the chapter highlights areas where self-management is failing so that changes can be made in care in advance of health deterioration
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