3 research outputs found
Evaluation of hydrogel formulated with seaweed extract (Ulva lactuca) for incised wound healing in white rats (Rattus norvegicus)
No full textSkin, as the largest organ in the human and animal body, serves as the main protection against various external factors such as sharp objects, extreme temperatures, chemicals, or physical trauma. This study aims to evaluate the effectiveness of seaweed (Ulva lactuca) extract-based hydrogel in accelerating incision wound healing in Rattus norvegicus rats. This experimental study used a post-test only controlled group design with six treatment groups: Ulva lactuca hydrogel 5%, 10%, 15%, positive control (Bioplasenton), negative control (wound without treatment), and rat group without treatment. Data were analyzed using ANOVA test. The results showed that Ulva lactuca hydrogel 5% gave the best results in accelerating wound healing. The wound diameter in the 5% group decreased significantly, from 15.3 cm on day 0 to 10.9 cm on day 3, 4.7 cm on day 6, and 2.36 cm on day 14. Histopathology results showed increased epithelialization, decreased inflammation, and increased collagen deposition in the 5% group compared with the control group. Quantitatively, the 5% hydrogel group achieved wound healing up to 30% faster than the positive control and 50% faster than the negative control. The 5% Ulva lactuca hydrogel proved to be most effective in accelerating wound healing by modulating inflammation and promoting tissue regeneration. This concentration provides an optimal balance between efficacy and safety, making it a potential alternative for wound therapy
UJI TOKSISITAS AKUT EKSTRAK KUNYIT PUTIH (CURCUMA ZEDOARIA) TERHADAP GAMBARAN HISTOPATOLOGI PANKREAS
No full textKunyit putih (Curcuma zedoaria) banyak digunakan oleh masyarakat luas karena dipercayai sebagai tanaman herbal yang bermanfaat dalam mengobati bermacam penyakit. Curcumin yang merupakan salah satu kandungan kunyit putih diyakini mempunyai manfaat sebagai antioxidan, anti inflamasi, anti mikroba, anti kanker, anti aterosklerosis dan anti diabetes. Secara umum, kepercayaan masyarakat tehadap penggunaan kunyit putih sebagai pengobatan herbal memiliki efek samping yang minimal jika dibandingkan dengan obat modern, namun penelitian mengenai efek samping dari penggunaan kunyit putih belum banyak diteliti. Tujuan dari penelitian ini adalah agar mengetahui uji toksisitas akut ekstrak kunyit putih (Curcuma zedoaria) terhadap gambaran histopatologi pankreas. Penelitian ini merupakan penelitian eksperimental yang menggunakan design Post Test Only Control Group Design dengan 30 ekor Rattus norvegicus jenis kelamin jantan dengan berat berkisar antara 150-300 gram. Tikus putih dibagi menjadi kontrol negatif aquades dan kontrol positif NaCMC 1%, dan 4 kelompok perlakuan ekstrak kunyit putih dengan dosis 250 mg/KgBB, 500 mg/KgBB, 750 mg/KgBB, dan 2000 mg/KgBB. Hasil yang didapat bahwa ekstrak kunyit putih (Curcuma zedoaria) tidak menunjukkan efek toksisitas akut pada pancreas tikus putih berupa tidak adanya nekrosis, edema dan sel radang pada gambaran histologi pankreas.
Isolation and enzyme bioprospection of bacteria associated to Bruguiera cylindrica, a mangrove plant of North Sumatra, Indonesia
No full textMangrove-associated bacteria are of industrial interest due to their diverse and versatile enzyme properties. This study investigates the culturable bacteria from a wide range of habitat in a Bruguiera cylindrica mangrove ecosystem in North Sumatra. Screening of extracellular hydrolytic enzymes showed multiple potential traits in amylase, cellulase, chitinase, phosphatase, protease, and urease production by bacterial isolates. Molecular identification based on 16S rDNA region of a potential strain, Vibrio alginolyticus Jme3−20 is then reported as a newly proteolytic agent. The strain also showed a stable growth under salinity (NaCl) stress with considerable phosphate solubilization activities. Protease activity was enhanced by optimizing the 0.5 % (w/v) sucrose and soy peptone in the fermentation medium. SDS-PAGE and zymogram analysis showed the presence of a 35-kDa MW protease. Hence, our study revealed important insights into the bacterial diversity and activity in mangrove ecosystems, evidencing the importance of microbial exploration in this ecosystem.Peer reviewe
