680 research outputs found
F.R. TOMLINSON: MENS EN WETENSKAPLIKE
No full textThe 1996 F.R. Tomlinson Memorial Lecture was delivered on 23rd May 1996 in Bloemfontein. The author, Prof D.J.G. Smith, chose the subject : F.R. Tomlinson: Man and ScientistTeaching/Communication/Extension/Profession,
F.R. Falconer's House
No full textPhotograph - A man in the backyard of F.R. Falconer's house, Athabasca, Alberta. Across the lane is the nurse's residence, later owned by Mrs. Dan Bilid
Detection Of An Untyped Strain Of Bovine Respiratory Syncytial Virus In A Dairy Herd
No full textBovine respiratory syncytial virus (BRSV) causes important lower respiratory tract illness in calves. According to F and G proteins genetic sequences, three BRSV subgroups have been reported and characterized in several countries, showing differences in its distribution. In Brazil, the virus is widely disseminated throughout the herds and the few characterized isolates revealed the solely occurrence of the subgroup B. This study describes the detection and characterization of an untyped BRSV strain from a twenty-days-old calf from a herd without clinical respiratory disease. Nasal swabs were analyzed by RT-nested PCR for the F and G proteins genes. One sample has amplified the F protein gene. Sequencing and subsequent phylogenetic reconstruction were accomplished, revealing that the strain could not be grouped with any other BRSV subgroups reported. This result may suggest that the BRSV is in constantly evolution, even in Brazil, where the vaccination is not a common practice. More detailed studies about BRSV characterization are necessary to know the virus subgroups distribution among the Brazilian herds to recommend appropriated immunoprophylaxis.35525392550Affonso, I.B., Gatti, S.P., Alexandrino, B., Oliveira, M.C., Medeiros, A.S.R., Buzinaro, M.G., Samara, S.I., Detection of antibodies against bovine respiratory syncytial virus (BRSV) in dairy cattle with different prevalences of bovine herpesvirus type 1 (BHV-1) in São Paulo State, Brazil (2011) Semina: Ciências Agrárias, 32 (1), pp. 295-300. , LondrinaAlmeida, R.S., Domingues, H.G., Spilki, F.R., Larsen, L.E., Hagglund, S., Belák, S., Arns, C.W., Circulation of bovine respiratory syncytial virus in Brazil (2006) Veterinary Record, 158 (18), pp. 632-634. , LondonAlmeida, R.S., Spilki, F.R., Roehe, P.M., Arns, C.W., Detection of Brazilian bovine respiratory syncytial virus strain by a reverse transcriptase-nestedpolymerase chain reaction in experimentally infected calves (2005) Veterinary Microbiology, 105 (2), pp. 131-135. , AmsterdamArns, C.W., Campalans, J., Costa, S.C.B., Domingues, H.G., D'Arce, R.C.F., Almeida, R.S., Characterization of bovine respiratory syncytial virus isolated in Brazil (2003) Brazilian Journal of Medical and Biological Research, 36 (2), pp. 213-218. , Ribeirão PretoBaker, J.C., Frey, M., Bovine respiratory syncytial virus (1985) Veterinary Clinics of North America: Food Animal Practice, 1 (2), pp. 259-272. , PhiladelphiaBidokhti, M.R.M., Travén, M., Ohlson, A., Zarnegar, B., Baule, C., Belák, S., Alenius, S., Liu, L., Phylogenetic analysis of bovine respiratory syncytial viruses from recent outbreaks in feedlot and dairy cattle herds (2012) Archives of Virology, 157 (4), pp. 601-607. , New YorkBunt, A.A., Milne, R.G., Sayaya, T., Verbeek, M., Vetten, H.J., Walsh, J.A., Paramyxoviridae (2005) Virus Taxonomy, Eigth Report of the International Committee on Taxonomy of Viruses, pp. 655-671. , In: FAUQUET, C. M.MAYO, M. A.MANILOFF, J.DESSELBERGER, U.BALL, L. A. (Ed.). London: Elsevier: Academic PressCampalans, J., Arns, C.W., Serological evidence of bovine respiratory syncytial virus in Brazil (1997) Virus Reviews and Research, 2 (1-2), pp. 50-56. , Belo HorizonteDomingues, H.G., Spilki, F.R., Arns, C.W., Detecção molecular e análise filogenética de vírus respiratório sincicial bovino (BRSV) em swabs e tecido pulmonar de bovinos adultos (2011) Pesquisa Veterinária Brasileira, 31 (11), pp. 961-966. , Rio de JaneiroFurze, J.M., Roberts, S.R., Wertz, G.W., Taylor, G., Antigenically distinct G glycoproteins of BRSV strains share a high degree of genetic homogeneity (1997) Virology, 231 (1), pp. 48-58. , New YorkFurze, J., Wertz, G., Lerch, R., Taylor, G., Antigenic heterogeneity of the attachment protein of bovine respiratory syncytial virus (1994) Journal of General Virology, 75 (2), pp. 363-370. , LondonGonçalves, I.P.D., Simanke, A.T., Jost, H.C., Hötzel, I., Dal Soglio, A., Moojen, V., Detection of bovine respiratory syncytial virus in calves of Rio Grande do Sul, Brazil (1993) Ciência Rural, 23 (3), pp. 389-390. , Santa MariaHall, T.A., BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT (1999) Nucleic Acids Symposium Series, 41, pp. 95-98. , OxfordLarsen, L.E., Bovine respiratory syncytial virus (BRSV): A review (2000) Acta Veterinaria Scandinavica, 41 (1), pp. 1-24. , CopenhagenLarsen, L.E., Tjornehoj, K., Viuff, B., Extensive sequence divergence among bovine respiratory syncytial viruses isolated during recurrent outbreaks in closed herds (2000) Journal of Clinical Microbiology, 38 (11), pp. 4222-4227. , WashingtonNettleton, P.F., Gilray, J.A., Caldow, G., Gidlow, J.R., Durkovic, B., Vilcek, S., Recent isolates of Bovine respiratory syncytial virus from Britain are more closely related to isolates from USA than to earlier British and current mainland European isolates (2003) Journal of Veterinary Medicine Series B-Infectious Diseases and Veterinary Public Health, 50 (4), pp. 196-199. , BerlinProzzi, D., Walravens, K., Langedijk, J.P., Daus, F., Kramps, J.A., Letesson, J.J., Antigenic and molecular analyses of the variability of bovine respiratory syncytial virus G glycoprotein (1997) Journal of General Virology, 78 (2), pp. 359-366. , LondonSchrijver, R.S., Daus, F., Kramps, J.A., Langedijk, J.P.M., Buijs, R., Middel, W.G.J., Taylor, G., Van Oirschot, J.T., Subgrouping of bovine respiratory syncytial virus strains detected in lung tissue (1996) Veterinary Microbiology, 53 (3-4), pp. 253-260. , AmsterdamSchrijver, R.S., Langedijk, J.P.M., Poel, V.D.M.W.H., Middel, W.G.J., Kramps, J.A., Van Oirschot, J.T., Antibody responses against the G and F proteins of bovine respiratory syncytial virus after experimental and natural infections (1996) Clinical and Diagnostic Laboratory Immunology, 3 (5), pp. 500-506. , WashingtonSchrijver, R.S., Langedijk, J.P.M., Middel, W.G.J., Kramps, J.A., Rijsewijk, F.A.M., Van Oirschot, J.T., A bovine respiratory syncytial virus strain with mutations in subgroup-specific antigenic domains of the G protein induces partial heterologous protection in cattle (1998) Veterinary Microbiology, 63 (2-4), pp. 159-175. , AmsterdamSpilki, F.R., Almeida, R.S., Domingues, H.G., D'Arce, R.C.F., Ferreira, H.L., Campalans, J., Costa, S.C.B., Arns, C.W., Phylogenetic relationships of Brazilian bovine respiratory syncyctial virus isolates and molecular homology modeling of attachment glycoprotein (2006) Virus Research, 116 (1-2), pp. 161-168. , AmsterdamSpilki, F.R., Arns, C.W., Vírus respiratório sincicial bovino (2008) Acta Scientiae Veterinariae, 36 (3), pp. 197-214. , Porto AlegreStine, L.C., Hoppe, D.K., Clayton, L.K., Sequence conservation in attachment glycoproteins and antigenic diversity among bovine respiratory syncytial virus isolates (1997) Veterinary Microbiology, 54 (3-4), pp. 201-221. , AmsterdamTamura, K., Dudley, J., Nei, M., Kumar, S., MEGA 4: Molecular evolutionary genetics analysis (MEGA) software version 4.0 (2007) Molecular Biology and Evolution, 24 (8), pp. 1596-1599. , ChicagoTaylor, G., Stott, E.J., Furze, J., Ford, J., Sopp, P., Protective epitopes on the fusion protein of respiratory syncytial virus recognized by murine and bovine monoclonal antibodies (1992) Journal of General Virology, 73 (9), pp. 2217-2223. , LondonTaylor, G., Thomas, L.H., Furze, J.M., Cook, R.S., Wyld, S.G., Lerch, R., Hardy, R., Wertz, G.W., Recombinant vaccinia viruses expressing the F, G or N, but not the M2, protein of bovine respiratory syncytial virus (BRSV) induce resistance to BRSV challenge in the calf and protect against the development of pneumonic lesions (1997) Journal of General Virology, 78 (12), pp. 3195-3206. , LondonThomas, L.H., Cook, R.S., Wyld, S.G., Furze, J.M., Taylor, G., Passive protection of gnotobiotic calves using monoclonal antibodies directed at different epitopes on the fusion protein of bovine respiratory syncytial virus (1998) Journal of Infectious Diseases, 177 (4), pp. 874-880. , ChicagoThompson, J.D., Higgins, D.G., Gibson, T.J., Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gag penalties and weight matrix choice (1994) Nucleic Acids Research, 22 (22), pp. 4673-4680. , OxfordValarcher, J.F., Schelcher, F., Bourhy, H., Evolution of bovine respiratory syncytial virus (2000) Journal of Virology, 74 (22), pp. 10714-10728. , WashingtonValarcher, J.F., Taylor, G., Bovine respiratory syncytial virus infection (2007) Veterinary Research, 38 (2), pp. 153-180. , Les UlisPoel, V.D.W., Brand, A., Kramps, J.A., Van Oirschot, J.T., Respiratory syncytial virus infections in human beings and in cattle (1994) Journal of Infectious Diseases, 29 (2), pp. 215-228. , ChicagoVilcek, S., Elvander, M., Ballagi-Pordány, A., Belák, S., Development of nested PCR assays for detection of bovine respiratory syncytial virus in clinical samples (1994) Journal of Clinical Microbiology, 32 (9), pp. 2225-2231. , WashingtonWoelk, C.H., Holmes, E.C., Variable immune-driven natural selection in the attachment (G) glycoprotein of respiratory syncytial virus (RSV) (2001) Journal of Molecular Evolution, 52 (2), pp. 182-192. , ChicagoYaegashi, G., Seimiya, Y.M., Seki, Y., Tsunemitsu, H., Genetic and antigenic analyses of bovine respiratory syncytial virus detected in Japan (2005) Journal of Veterinary Medical Science, 67 (2), pp. 145-150. , Toky
Molecular Detection And Phylogenetic Analysis Of Bovine Respiratory Syncytial Virus (brsv) In Swabs And Lung Tissues Of Adult Cattle [detecção Molecular E Análise Filogenética De Vírus Respiratório Sincicial Bovino (brsv) Em Swabs E Tecido Pulmonar De Bovinos Adultos]
No full textBovine respiratory syncytial viruses virus (BRSV) is one of the etiologic agents of pneumonia in young cattle. Few studies have been made aiming detection of the virus in samples collected from adult animals, especially those asymptomatic bovines. However, it is assumed that infections in these groups may occur mostly asymptomatic and this would be an important mechanism for maintaining of BRSV in herds. In this study, the goal was to conduct an analysis of the occurrence of asymptomatic infections by BRSV in lung samples (n=68) and nasal swabs (209) taken from adult animals collected in abattoirs from Southern and Southeastern Brazil respectively, to detect via polymerase chain reaction the occurrence of infected animals in populations of adult cattle. The samples that resulted positive (6) on RT-PCR were subsequently subjected to cutting with restriction enzymes and sequencing for genetic characterization (2 samples). All samples belongs to subgroup B of BRSV, which is reported as the one circulating in Brazil. The results obtained demonstrate that BRSV may be present in samples taken from adult animals, which is in agreement the hypothesis that infections in adults run in a sub-clinical way that may be of importance as a maintenance mechanism of the virus in bovine herds.3111961966Almeida, R.S., Spilki, F.R., Roehe, P.M., Arns, C.W., Detection of Brazilian bovine respiratory syncytial virus strain by a reverse transcriptase- nested-polymerase chain reaction in experimentally infected calves (2005) Vet. Microbiol., 105, pp. 131-135Antonis, A.F.G., Claassen, E.A.W., Hensen, E.J., Groot, R.J.D., Groot-Mijnes, J.D.F.D., Schrijver, R.S., Van Der Most, R.G., Kinetics of antiviral C D8 T cell responses during primary and post-vaccination secondary bovine respiratory syncytial virus infection (2006) Vaccine, 24, pp. 1551-1561Arns, C.W., Campalans, J., Costa, S.C.B., Domingues, H.G., D'Arce, R.C.F., Almeida, R.S., Characterization of bovine respiratory syncytial virus is olated in Brazil (2003) Braz. J. Med. Biol. Res., 36, pp. 213-218Beaudeau, F., Björkman, C., Alenius, S., Frössling, J., Spatial patterns of Bovine Corona Virus and Bovine Respiratory Syncytial Virus in the Swe dish beef cattle population (2010) Acta Vet. Scand., , Article 52Brodersen, B.W., Bovine respiratory syncytial virus (2010) Vet. Clin. North Am., Food Anim. Pract., 26, pp. 323-333De Jong, M.C.M., Van Der Poel, W.H.M., Kra mps, J.A., Brand, A., Van Oirschot, J.T., Quantitative investigation of population persistence and recurre nt outbreaks of bovine respiratory syncytial virus on dairy farms (1996) Am. J. Vet. Res., 57, pp. 628-633Domingues, H.G., Campalans, J., Almeida, R.S., Coswig, L.T., Arns, C.W., Dot-enzyme linked immunosorbent assay as an alternative technique for the d etection of bovine respiratory syncytial virus (BRSV) antibodies (2002) Vet. Res., 33, pp. 397-404Duncan Jr., R.B., Potgieter, L.N.D., Antigenic diversity of respiratory syncytial viruses and its implication for immunoprophylaxis in ruminants (1993) Vet. Microbiol., 37, pp. 319-341Easton, A.J., Domachowske, J.B., Rosenberg, H.F., Animal pneumoviruses: Molecular genetics and pathogenesis (2004) Clin. Microbiol. Rev., 17, pp. 390-412Ellis, J.A., Update on viral pathogenesis in BRD (2009) Animal Health Research Reviews/Conference of Research Workers in Animal Diseases, 10, pp. 149-153Furze, J., Wertz, G., Lerch, R., Taylor, G., Antigenic heterogeneity of the attachment protein of bovine respiratory syncytial virus (1994) J. General Vi rol., 75, pp. 363-370Gershwin, L.J., Bovine respiratory syncytial virus infection: Immunopathogenic mechanisms (2007) Animal Health Research Reviews/Conference of Research Workers in Animal Diseases, 8, pp. 207-213Gershwin, L.J., Berghaus, L.J., Arnold, K., Anderson, M.L., Corbeil, L.B., Immune mechanisms of pathogenetic synergy in concurrent bovine pulmonary infection with Haemophilus somnus and bovine respiratory syncytial virus (2005) Vet. Immunol. Immunopathol., 107, pp. 119-130Gershwin, L.J., Dungworth, D.L., Himes, S.R., Friebertshauser, K.E., Immunoglobulin E responses and lung pathology resulting from aerosol exposure of calves to respiratory syncytial virus and Micropolyspora faeni (1990) Int. Archs Allergy Appl. Immunol., 92, pp. 293-300Gershwin, L.J., Gunther, R.A., Hornof, W.J., Larson, R.F., Effect of infection with bovine respiratory syncytial virus on pulmonary clearance of an inhaled antigen in calves (2008) Am. J. Vet. Res., 69, pp. 416-422Gulliksen, S.M., Jor, E., Lie, K.I., Løken, T., Åkerstedt, J., ØsterÅs, O., Respiratory infections in Norwegian dairy calves (2009) J. Dairy Sci., 92, pp. 5139-5146Hägglund, S., Hjort, M., Graham, D.A., Öhagen, P., Törnquist, M., Alenius, S., A six-year study on respiratory viral infections in a bull testing facility (2007) Vet. Journal, 173, pp. 585-593Hägglund, S., Svensson, C., Emanuelson, U., Valarcher, J.F., Alenius, S., Dynamics of virus infections involved in the bovine respiratory disease complex in Swedish dairy herds (2006) Vet. Journal, 172, pp. 320-328Kalina, W.V., Anderson, M.L., Gershwin, L.J., Alternaria aerosol during a bovine respiratory syncytial virus infection alters the severity of subsequent re -infection and enhances IgE production (2006) Comp. Immunol. Microbiol. Infect. Dis., 29, pp. 138-156Larsen, L.E., Tjørnehøj, K., Viuff, B., Jensen, N.E., Uttenthal Å, Diagnosis of enzootic pneumonia in Danish cattle: Reverse transcription- -polymerase chain reaction assay for detection of bovine respiratory syncytial virus in naturally and experimentally infected cattle (1999) J. Vet. Diagn. Invest., 11, pp. 416-422Larsen, L.E., Uttenthal, A., Arctander, P., Tjørnehøj, K., Viuff, B., Røntved, C., Rønsholt, L., Blixenkrone-Møller, M., Serological and genetic c haracterisation of bovine respiratory syncytial virus (BRSV) indicates that Danish isolates belong to the intermediate subgroup: No evidence of a selective effect on the variability of G protein nucleotide sequence by prior cell culture adaption and passages in cell culture or calves (1998) Vet. Microbiol., 62, pp. 265-279Nettleton, P.F., Gilray, J.A., Caldow, G., Gidlow, J.R., Durkovic, B., Vilcek, S., Recent isolates of Bovine respiratory syncytial virus from Britain are more closely related to isolates from USA than to earlier British and current mainland European isolates (2003) J. Vet. Med. B, 50, pp. 196-199Paccaud, M.F., Jacquier, C., A Respiratory syncytial virus of bovine origin (1970) Archiv für die gesamte Virusforschung, 30, pp. 327-342Schrijver, R.S., Daus, F., Kramps, J.A., Langedijk, J.P.M., Buijs, R., Middel, W.G.J., Taylor, G., Van Oirschot, J.T., Subgrouping of bovine re spiratory syncytial virus strains detected in lung tissue (1996) Vet. Microbiol., 53, pp. 253-260Schrijver, R.S., Langedijk, J.P.M., Middel, W.G.J., Kramps, J.A., Rijsewijk, F.A.M., Van Oirschot, J.T., A bovine respiratory syncytial virus strain with mutations in subgroup-speciic antigenic domains of the G protein induces partial heterologous protection in cattle (1998) Vet. Microbiol., 63, pp. 159-175Schrijver, R.S., Langedijk, J.P.M., Van Der Poel, W.H.M., Middel, W.G.J., Kramps, J.A., Van Oirschot, J.T., Antibody responses against the G and F proteins of bovine respiratory syncytial virus after experimental and natural infections (1996) Clin. Diagn. Lab. Immunol., 3, pp. 500-506Spilki, F.R., Almeida, R.S., Domingues, H.G., D'Arce, R.C.F., Ferreira, H.L., Campalans, J., Costa, S.C.B., Arns, C.W., Phylogenetic relation ships of Brazilian bovine re spiratory syncytial virus isolates and molecular homology modeling of attachment glycoprotein (2006) Virus Res., 116, pp. 30-37Spilki, F.R., de Almeida, R.S., Arns, C.W., Antibody responses in mice after immunization with inactivated bovine respiratory syncytial virus using different adjuva nts (2010) Cîncia Rural, 40, pp. 2332-2337Tamura, K., Dudley, J., Nei, M., Kumar, S., MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0 (2007) Molecular Biology and Evolution, 24, pp. 1596-1599Thompson, J.D., Gibson, T.J., Higgins, D.G., (2002) Multiple sequence alignment using ClustalW and Clustal X, , Current Protocols in Bioinformatics/Editoral Board, Andreas D. Baxevanis... 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Cloning Of The Transmembrane Glycoproteins G And F From A Brazilian Isolate Of Bovine Respiratory Syncytial Virus In A Prokaryotic System
No full textThe aim of this work was the cloning of those transmembrane glycoproteins G and F from an isolate bovine respiratory syncytial viruses (BRSV) - a Brazilian isolate of BRSV, named BRSV-25-BR in previous studies, in a prokaryotic system to proceed the sequencing of larger genomic fragments. The nucleotide substitutions were confirmed and these clones may also be used in further studies regarding the biological effects of those proteins in vitro and in vivo.633552558Almeida, R.S., Spilki, F.R., Roehe, P.M., Detection of Brazilian bovine respiratory syncytial virus strain by a reverse transcriptase-nested-polymerase chain reaction in experimentally infected calves (2005) Vet. Microbiol., 105, pp. 131-135Arns, C.W., Campalans, J., Costa, S.C.B., Characterization of bovine respiratory syncytial virus isolated in Brazil (2003) Braz. J. Med. Biol. Res., 36, pp. 213-218Bastien, N., Taylor, G., Thomas, L.H., Immunization with a peptide derived from the G glycoprotein of bovine respiratory syncytial virus (BRSV) reduces the incidence of BRSV associated pneumonia in the natural host (1997) Vaccine, 15, pp. 1385-1390Buchholz, U.J., Granzow, H., Schuldt, K., Chimeric bovine respiratory syncytial virus with glycoprotein gene substitutions from human respiratory syncytial virus (hRSV): effects on host range and evaluation as a live-attenuated hRSV vaccine (2000) J. Virol., 74, pp. 1187-1199Collins, P.L., Chanock, R.M., Murphy, B.R., Respiratory syncytial virus (2001) Fields virology, pp. 1443-1486. , Knipe, D.M.Howley, P.M. (Eds.). , 4.ed. 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Virol., 82, pp. 631-640Langedijk, J.P., Schaaper, W.M., Meleon, R.H., Proposed three-dimensional model for the attachment protein G of respiratory syncytial virus (1996) J. Gen. Virol., 77, pp. 1249-1257Langedijk, J.P., Meleon, R.H., Taylor, G., Antigenic structure of the central conserved region of protein G of bovine respiratory syncytial virus (1997) J. Virol, 243, pp. 293-302Larsen, L.E., Bovine respiratory syncytial virus (BRSV): a review (2000) Acta Vet. Scand., 41, pp. 1-21Lerch, R.A., Anderson, K., Wertz, G.W., Nucleotide sequence analysis and expression from recombinant vectors demonstrate that the attachment protein G of bovine respiratory syncytial virus (1990) J. Virol., 64, pp. 5559-5569Levine, S., Klaiber Franco, R., Paradiso, P.R., Demonstration that glycoprotein G is the attachment protein of respiratory syncytial virus (1987) J. Gen. 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Immunol., 3, pp. 500-506Spilki, F.R., Almeida, R.S., Domingues, H.G., Phylogenetic relationships of Brazilian bovine respiratory syncytial virus isolates and molecular homology modeling of attachment glycoprotein (2006) Virus Res., 116, pp. 30-37Spilki, F.R., Almeida, R.S., Campalans, J., Susceptibility of different cell lines to infection with bovine respiratory syncytial virus (2006) J. Virol. Methods, 131, pp. 130-133Spilki, F.R., Almeida, R.S., Ferreira, H.L., Effects of experimental inoculation of bovine respiratory syncytial virus in different inbred mice lineages: Establishment of a murine model for BRSV infection (2006) Vet. Microbiol., 118, pp. 161-168Stine, L.C., Hoppe, D.K., Kelling, C.L., Sequence conservation in the attachment glycoprotein and antigenic diversity among bovine respiratory syncytial virus isolates (1997) Vet. Microbiol., 54, pp. 201-221Taylor, G., Stott, E.J., Furze, J., Protective epitopes on the fusion protein of respiratory syncytial virus recognized by murine and bovine monoclonal antibodies (1992) J. Gen. Virol., 73, pp. 2217-2223Taylor, G., Thomas, L.H., Furze, F.A.M., Recombinant vaccinia viruses expressing the F, G or N, but not the M2, protein of bovine respiratory syncytial virus (BRSV) induce resistance to BRSV chalengee in the calf and protest against the development of pneumonic lesions (1997) J. Gen. Vilrol., 78, pp. 3195-3206Taylor, G., Rijsewijk, F.A.M., Thomas, L.H., Resistance to bovine respiratory syncytial virus (BRSV) induced in calves by a recombinant bovine herpesvirus-1 expressing the attachament glycoprotein of BRSV (1998) J. Gen. Virol., 79, pp. 1759-1767Taylor, G., Bruce, C., Barbet, A.F., DNA vaccination against respiratory syncytial virus in young calves (2005) Vaccine, 23, pp. 1242-1250Techaarpornkul, S., Barretto, N., Peeples, M.E., Functional analysis of recombinant respiratory syncytial virus deletion mutants lacking the small hydrophobic and/or attachment glycoprotein gene (2001) J. Virol., 75, pp. 6825-6834Teng, M.N., Collins, P.L., The central conserved cystine noose of the attachment G protein of human respiratory syncytial virus is not required for efficient viral infection in vitro or in vivo (2002) J. Virol., 76, pp. 6164-6171Thomas, L.H., Cook, R.S., Wyld, S.G., Passive protection of gnotobiotic calves using monoclonal antibodies at different epitopes on the fusion protein of bovine respiratory syncytial virus (1998) J. Infect. Dis., 177, pp. 874-880Thompson, J.D., Higgins, D.G., Gibson, T.J., Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice (1994) Nucleic Acids Res., 22, pp. 4673-4680Vainionpää, R., Marusyk, R., Salmi, A., The Paramyxoviridae aspects of molecular structure, pathogenesis, and immunity (1989) Adv. Virus Res., 37, pp. 211-242Valarcher, J.F., Schelcher, F., Bourhy, H., Evolution of bovine respiratory syncytial virus (2000) J. Virol., 74, pp. 10714-10728Valarcher, J.F., Taylor, G., Bovine respiratory syncytial virus infection (2007) Vet. Res., 38, pp. 153-180Valentova, V., The antigenic and genetic variability of bovine respiratory syncytial virus with emphasis on the G protein (2003) Med., Czech, 48, pp. 254-266Wertz, G.W., Collins, P.L., Huang, Y., Nucleotide sequence of the G protein gene of human respiratory syncytial virus reveals an unnsual type of viral membrane protein (1985) Proc. Natl. Acad. Sci., USA, 82, pp. 4075-407
A Brazilian glycoprotein E-negative bovine herpesvirus type 1.2a (BHV-1.2a) mutant is attenuated for cattle and induces protection against wild-type virus challenge
No full textThe authors previously reported the construction of a glycoprotein E-deleted (gE-) mutant of bovine herpesvirus type 1.2a (BHV-1.2a). This mutant, 265gE-, was designed as a vaccinal strain for differential vaccines, allowing the distinction between vaccinated and naturally infected cattle. In order to determine the safety and efficacy of this candidate vaccine virus, a group of calves was inoculated with 265gE-. The virus was detected in secretions of inoculated calves to lower titres and for a shorter period than the parental virus inoculated in control calves. Twenty one days after inoculation, the calves were challenged with the wild type parental virus. Only mild signs of infection were detected on vaccinated calves, whereas non-vaccinated controls displayed intense rhinotracheitis and shed virus for longer and to higher titres than vaccinated calves. Six months after vaccination, both vaccinated and control groups were subjected to reactivation of potentially latent virus. The mutant 265gE- could not be reactivated from vaccinated calves. The clinical signs observed, following the reactivation of the parental virus, were again much milder on vaccinated than on non-vaccinated calves. Moreover, parental virus shedding was considerably reduced on vaccinated calves at reactivation. In view of its attenuation, immunogenicity and protective effect upon challenge and reactivation with a virulent BHV-1, the mutant 265gE- was shown to be suitable for use as a BHV-1 differential vaccine viru
Forms of judaism in writings of F.R. Kraus, František Kafka, Hana Bělohradská and J.R. Pick
No full textTéma této práce zní Podoby židovství v dílech F.R. Krause, Františka Kafky, Hany Bělohradské a J.R. Picka. Soustředila jsem se na méně známé české autory, kteří se ve své tvorbě věnovali tématu židovství. Jedná se o texty související s 2. světovou válkou. V úvodu je nastíněn stručný přehled o působení Židů na našem území, následuje představení zvolené čtveřice autorů. Hlavním cílem bylo přiblížit a rozebrat vybraná díla a v závěru shrnout jejich odlišné a podobné znaky. Současně byly tyto texty zařazeny do kontextu české prózy s tematikou okupace.Katedra českého jazyka a literaturyObhájenoThe theme of this bachelor work is called Forms of judaism in writings of F.R. Kraus, František Kafka, Hana Bělohradská and J.R. Pick. In this thesis the author tried to concentrate on a few less-known Czech writers, who devoted their writing to the fate of Jews during the Second World War. In the first part of this work the author outlined the influence of the Jewish etnic group on the population of our country, with the aim to gain orientation in the historical backgound of that time. Next the author analysed the texts of selected books with the Jewish theme from different points of view and in the end of her work compared the books she used. In the closing of this work there is a contextual classification of the mentioned books into the category of works with the topic of occupation, from the end of the Second World War until today
Antibody Responses In Mice After Immunization With Inactivated Bovine Respiratory Syncytial Virus Using Different Adjuvants [respostas De Anticorpos Em Camundongos Após Imunização Com Vírus Respiratório Sincicial Bovino Inativado Utilizando Diferentes Adjuvantes]
No full textBovine respiratory syncytial virus (BRSV) causes pneumonia in young cattle. Modified-live-virus (MLV) and inactivated vaccines are currently used for the control of clinical effects of BRSV infections in cattle. On the present research, the stimulation of specific anti-BRSV immunoglobulin isotypes was investigated, through the use of different commercially available adjuvants (Water-in-oil emulsion, Quil A, Aluminumhydroxide) in inbred mice (Balb/C and C57BL/6). BRSV antibodies were measured using an enzyme-linked immunosorbent assay (ELISA) and the results were compared to the antibody levels induced by immunization of animals using live-BRSV-virus. Water-in-oil emulsion and alumadjuvant preparations induced higher levels of IgG1 immunoglobulins, whereas Quil A favored the production of IgG2 antibodies, this last being a more appropriate response profile for the specific case of BRSV. Not using adjuvants resulted in poor levels of specific antibodies. The isotype profile of specific antibodies obtained varied greatly depending on the adjuvants used. This information may be useful for the formulation of more effective BRSV inactivated vaccines; however, these findings have to be confirmed in cattle.401123322337Ayres, M., BioEstat 4.0: Aplicações estatísticas nas áreas das ciências biológicas e médicas (2005) Belém: Sociedade Civil Mamirauá, p. 290Corteling, R., Trifilieff, A., Gender comparison in a murine model of allergen-driven airway inflammation and the response to budesonide treatment BMC Pharmacology, 4, p. 4. , http://dx.crossref.org/10.1186%2F1471-2210-4-4, Available from, Accessed: Mar. 22, 2010. doi:10.1186/1471-2210-4-4Cox, J.C., Coulter, A.R., Adjuvants - a classification and review of their modes of action (1997) Vaccine, 15, pp. 248-256. , http://dx.doi.org/10.1016/S0264-410X(96)00183-1, Available from, Accessed: Mar. 01, 2008. doi: 10.1016/S0264-410X(96)00183-1Ellis, J., Efficacy of an inactivated respiratory syncytial virus vaccine in calves (2001) Journal of American Veterinary Medical Association, 230, pp. 1973-1980. , http://dx.doi.org/abs/10.2460/javma.2001.218.1973, Available from, Accessed: Mar. 01, 2008. doi: 10.2460/javma.2001.218.1973Ellis, J., Bovine respiratory syncytial virus-specific immune responses in cattle following immunization with modified-live and inactivated vaccines. Analysis of the specificity and activity of serum antibodies (1995) Veterinary Immunology and Immunopathology, 34, pp. 35-45. , http://dx.doi.org/10.1016/0165-2427(92)90150-O, Available from, Accessed: Jun. 06, 2009. doi: 10.1016/0165-2427(92)90150-OEllis, J., Efficacy of a saponin-adjuvanted inactivated respiratory syncytial virus vaccine in calves (2005) Canadian Veterinary Journal, 46, pp. 155-162. , http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1082864/, Available from, Accessed: Abr. 20, 2010Flores, E.F., Donis, R.O., Isolation of a mutant MDBK cell line resistant to bovine viral diarrhea virus infection due to a block in viral entry (1995) Virology, 208, pp. 565-575. , http://dx.doi.org/10.1006/viro.1995.1187, Available from, Accessed: Mar. 01, 2008. doi: 10.1006/viro.1995.1187Gershwin, L.J., Bovine respiratory syncytial virus infection: Immunopathogenic mechanisms (2007) Animal Health Resesearch Reviews, 8, pp. 207-213. , http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=1660948, Available from, Accessed: Abr. 20, 2010. doi: 10.1017/S1466252307001405Gershwin, L.J., Immune mechanisms of pathogenetic synergy in concurrent bovine pulmonary infection with Haemophilus somnus and bovine respiratory syncytial virus (2005) Veterinary Immunology and Immunopathology, 107, pp. 119-130. , http://dx.doi.org/10.1016/j.vetimm.2005.04.004Hägglund, S., Bovine respiratory syncytial virus ISCOMs - protection in the presence of maternal antibodies (2004) Vaccine, 23, pp. 646-655. , http://dx.doi.org/10.1016/j.vaccine.2004.07.006, Available from, Accessed: Jun. 06, 2009. doi: 10.1016/j.vetimm.2005.04.004Kalina, W.V., Formalin-inactivated bovine RSV vaccine enhances a Th2 mediated immune response in infected cattle (2004) Vaccine, 22, pp. 1465-1474. , http://dx.doi.org/10.1016/j.vaccine.2003.10.024, Available from, Accessed: Abr. 20, 2010. doi: 10.1016/j.vaccine.2003.10.024Kerkhofs, P., et al, Immune responses to bovine respiratory syncytial virus (BRSV) following use of an inactivated BRSVPI3-Mannheimia haemolytica vaccine and a modified live BRSV-BVDV vaccine (2004) Veterinary Journal, 167, pp. 208-210. , http://dx.doi.org/10.1016/S1090-0233(03)00078-9, Available from, Accessed: Jun. 06, 2009. doi: 10.1016/S1090-0233(03)00078-9Kim, H.W., Respiratory syncytial virus disease in infants despite prior administration of antigenic inactivated vaccine (1969) American Journal of Epidemiology, 89, pp. 422-434. , http://aje.oxfordjournals.org/content/89/4/422.full.pdf+html, Available from, Accessed: Mar. 01Larsen, L.E., Bovine respiratory syncytial virus (BRSV) pneumonia in beef calf herds despite vaccination (2001) Acta Veterinaria Scandinavica, 42, pp. 113-121. , http://dx.crossref.org/10.1186%2F1751-0147-42-113, Available from, Accessed: Mar. 01, 2008. doi:10.1186/1751-0147-42-113Letellier, C., Vaccination of calves using the BRSV nucleocapsid protein in a DNA prime-protein boost strategy stimulates cell-mediated immunity and protects the lungs against BRSV replication and pathology (2008) Vaccine, 26, pp. 4840-4848. , http://dx.doi.org/10.1016/j.vaccine.2008.06.100, Available from, Accessed: Abr. 20, 2010. doi: 10.1016/j.vaccine.2008.06.100Mawhinney, I.C., Burrows, M.R., Protection against bovine respiratory syncytial virus challenge following a single dose of vaccine in young calves with maternal antibody (2005) Veterinary Record, 156, pp. 139-143. , http://veterinaryrecord.bvapublications.com/cgi/reprint/156/5/139, Available from, Accessed: Mar. 01, 2008Patel, J.R., Didlick, S.A., Evaluation of efficacy of an inactivated vaccine against bovine respiratory syncytial virus in calves with maternal antibodies (2004) American Journal of Veterinary Research, 65, pp. 417-421. , http://dx.doi.org/10.2460/ajvr.2004.65.417, Available from, Accessed: Mar. 01, 2008. doi: 10.2460/ajvr.2004.65.417Romera, S.A., Adjuvant effects of sulfolipo-cyclodextrin in a squalane-in-water and water-in-mineral oil emulsions for BHV-1 vaccines in cattle (2000) Vaccine, 19, pp. 132-141. , http://dx.doi.org/10.1016/S0264-410X(00)00104-3, Available from, Accessed: Mar. 01, 2008. doi: 10.1016/S0264-410X(00)00104-3Schreiber, P., High mortality rate associated with bovine respiratory syncytial virus (BRSV) infection in Belgian white blue calves previously vaccinated with an inactivated BRSV vaccine (2000) Journal Veterinary Medicine Series B, 47, pp. 535-550. , http://dx.doi.org/10.1046/j.1439-0450.2000.00380.x, Available from, Accessed: Mar. 01, 2008. doi: 10.1046/j.1439-0450.2000.00380.xSpilki, F.R., Susceptibility of different cell lines to infection with bovine respiratory syncytial virus Journal of Virological Methods, 131, pp. 130-133. , http://dx.doi.org/10.1016/j.jviromet.2005.08.002, Available from, Accessed: Mar. 01, 2008. doi: 10.1016/j.jviromet.2005.08.002Spilki, F.R., Effects of experimental inoculation of bovine respiratory syncytial virus in different inbred mice lineages: Establishment of a murine model for BRSV infection (2006) Veterinary Microbiology, 118, pp. 161-168. , http://dx.doi.org/10.1016/j.vetmic.2006.07.011, Available from, Accessed: Mar. 01, 2008. doi: 10.1016/j.vetmic.2006.07.011West, K., The effect of formalin-inactivated vaccine on respiratory disease associated with bovine respiratory syncytial virus infection in calves (1999) Vaccine, 17, pp. 809-820. , http://dx.doi.org/10.1016/S0264-410X(98)00265-5, Available from, Accessed: Mar. 01, 2008. doi: 10.1016/S0264-410X(98)00265-
Molecular detection and phylogenetic analysis of the gene H from canine distemper virus isolates circulating at the municipality of Campinas, Sao Paulo
No full textRosa G.N., Domingues H.G., Santos M.M.A.B., Felippe P.A.N., Spilki F.R. & Arns C. W. 2012. [Molecular detection and phylogenetic analysis of the gene H from canine distemper virus isolates circulating at the municipality of Campinas, Sao Paulo.] Deteccao molecular e analise filogenetica do gene H de amostras do virus da cinomose canina em circulacao no municipio de Campinas, Sao Paulo. Pesquisa Veterinaria Brasileira 32(1): 72-77. Laboratorio de Microbiologia Molecular, Instituto de Ciencias da Sa de, Universidade Feevale, Rodovia RS-239 2755, Novo Hamburgo, RS 93352-000, Brazil. E-mail: [email protected] Canine distemper virus (CDV), a Morbillivirus of the family Paramyxoviridae, is the etiological agent of neurological and systemic disease in dogs. The laboratory diagnosis of infection requires viral isolation or detection of genetic material of the virus in secretions or tissues of dogs with clinical suspicion of the disease. The genetic diversity among isolates of CDV can be assessed by sequencing and phylogenetic analysis of the gene that encodes the viral hemagglutinin (H gene), and there is currently a special interest in comparing the strains currently circulating in the field with the genogroup America-1, which comprises strains present in vaccines available in the market. In this study, the molecular detection of CDV gene H was performed from biological samples harvested ante-and post-mortem from 15 dogs with clinical signs suggestive of canine distemper in the metropolitan region of Campinas, Sao Paulo. Ten of the 15 dogs examined had at least one positive organ under molecular detection and the obtained amplicons were sequenced and further analyzed by molecular phylogenetic analysis. Similarly to what has already been reported on previous studies regarding the diversity of the gene H in other countries, the phylogenetic reconstruction obtained for the samples of cases of distemper from Campinas region showed they were grouped with the North American, European and Japanese newly described samples, a genetic group distinguished from classical samples of CDV, named America-1, which encompasses the vaccine strains Snyder Hill, Onderstepoort and Lederle..321727
Organization of shallow cumulus clouds
No full textHPCI User Report for the project Organization of shallow cumulus clouds on the Fugaku supercomputer.Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Atmospheric Remote Sensin
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