1,721,049 research outputs found

    The molecular composition of humus carbon: Recalcitrance and reactivity in soils

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    Soil organic C (SOC) is the largest terrestrial reservoir in the biosphere, accounting for 1500–1770 Pg, as compared to C stocks of vegetation (450– 650 Pg) (IPCC, 2013). Although humus C represents from 60% to 80% of SOC, its dynamics still remain poorly understood after nearly a century of study, due to the multiplicity of factors that affect stabilization of humic matter. Industrial agricultural practices accelerate the decline of humus content in soil, and, consequently, the reduction of soil fertility, biodiversity, and soil structural stability (Fontaine et al., 2007; Reeves, 1997), while enhancing greenhouse gases (GHG) emissions from soil (Smith et al., 2014). Because it is the specific molecular composition of the soil Humeome that significantly affects SOC storage dynamics (Woo et al., 2014), soil basal respiration (Fang et al., 2005), and humus-plant relationships (Canellas and Olivares, 2014), a rigorous identification of the molecular structure of the components of soil humus C is necessary, if any technological control of its content and dynamics can ever be introduced

    Antioxidant Features of Humic Products by ABTS Assay

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    Oxidative stress plays a pivotal role in driving immunosenescence by disrupting cellular homeostasis and impairing immune function. Humic substances exhibit scavenging activity against reactive oxygen species (ROS), inhibit ROS generation via metal chelation, and modulate endogenous antioxidant enzyme activity. Additionally, humic substances display anti-inflammatory effects, further supporting cellular redox balance. Given their antioxidant activity, humic substances hold promise as natural compounds for mitigating oxidative stress-associated immunosenescence. Here we describe the evaluation of antioxidant capacities of humic products by ABTS spectrophotometric assay

    Metabolomic by 1H NMR Spectroscopy Differentiates “Fiano Di Avellino” White Wines Obtained with Different Yeast Strains

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    We employed 1H NMR spectroscopy to examine the molecular profile of a white “Fiano di Avellino” wine obtained through fermentation by either a commercial or a selected autochthonous Saccharomyces cerevisiae yeast starter. The latter was isolated from the same grape variety used in the wine-making process in order to strengthen the relationship between wine molecular quality and its geographical origin. 1H NMR spectra, where water and ethanol signals were suppressed by a presaturated T1-edited NMR pulse sequence, allowed for definition of the metabolic content of the two differently treated wines. Elaboration of NMR spectral data by multivariate statistical analyses showed that the two different yeasts led to significant diversity in the wine metabolomes. Our results indicate that metabolomics by 1H NMR spectroscopy combined with multivariate statistical analysis enables wine differentiation as a function of yeast species and other wine-making factors, thereby contributing to objectively relate wine quality to the terroir
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