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Respon Imun Humoral Mencit yang Diberi Spirulina platensis dan Diinfeksi dengan Takizoit
No full textRegulasi osmotik dan nilai hematokrit ikan nila Oreochromis sp pada medium dengan salinitas dan temperatur air berbeda
No full textOsmotic regulation and blood hematocrite of nile, Oreochromis sp., have been evaluated as response to different salinity and water temperature. Three different salinities (0 ppt, 10 ppt and 20 ppt) in combination with two different water temperatures (26 ° C and 30 ° C) have been applicated in this experiment. Fish with average body weight 28.3 ± 2.62 g were used ini this experiment. The result showed that plasma osmolality of nile in freshwater have not different with in 10 ppt (P > 0.05), but plasma osmolality increased at 20 ppt especially at 8,16 and 24 hours after aclimated on 20 ppt. Osmoregulatory capacity has signifi cant heigher (P .05). Conclution, osmotic regulation of nile showed signifi cant different between salinity treatment, but blood hematocrite only changed at 8,and16 hours after nile aclimated on water salinity 20 ppt and body water content not changed at both diferent salinity and water temperature
Genetic Variation of Hampala Fish (Hampala macrolepidota) Population in PB. Soedirman Reservoir and Serayu River
Get PDFPanglima Besar Soedirman waters reservoir and the Serayu River in Banjarnegara Regency, Central Java is one of the habitats of hampala fish . Hampala fish is a member of the Cyprinidae family, which has economic value but is fully captured from the wild. The study on the genetic diversity using approaches of isozyme analysis needed to support conservation and domestication of the fish in this area. This study was aimed at the genetic variation of the hampala fish population in PB. Soedirman water reservoir and the Serayu River in Banjarnegara Regency based on esterase (EST), acid phosphatase (ACP), peroxidase (PER), and aspartate aminotransferase (AAT). Visualization of the isozyme was carried out employing horizontal electrophoretic technique with potato starch. From the results of this study it can be concluded that the hampala fish from the reservoir of PB. Soedirman, Serayu River area before reservoir and after reservoir, all of which are in Banjarnegara Regency, can visualized isozymes EST, ACP, and AAT well, except PER isozyme. This finding can be used as based information on population genetics and finally can be used for conservation of this fish. The results of this study are expected to be utilized to evaluate the potential genetic condition of hampala fish, which is the basis for conservation strategy and domestication.</jats:p
Stimulation of Deprivation Cycles with Spirulina platensis Feed Supplementation on Osphronemus gouramy Physiological Responses
Get PDFSpirulina platensis is a phytoplankton, the cell wall composed of complex sugars so easily digested by fish. The present study was carried out to investigate stimulation cycle of feed deprivation with feed supplemented S. platensis the best to increase growth, hematological and body composition of gurami (Osphronemus gouramy). Groups of 24 fish, each in triplicate, were exposed to four different treatment for a period of 56 days. Sample measurements of growth done every 14 days, hematological and body composition measurements carried out at the end of the experiment. Growth was significantly different between stimulation cycle of feed deprivation and the control (P<0.05). Conclusions result showed that stimulation cycles of feed deprivation could not improve growth and hematological, but could improve body composition. Feed deprivation is done to reduce the cost of production, high production costs due to high feed prices. During research on feed deprivation is done by giving commercial feed, this study is to provide feed supplementation S. platensis. Thus, the results of this study can be useful for science as S. platensis information can be used as a food supplement and and for the people cultivating gurami should be fed daily supplementation of S. platensis.How to CiteSimanjuntak, S. B. I., Wibowo, E. S. & Indarmawan, I. (2016). Stimulation of Deprivation Cycles with Spirulina platensis Feed Supplementation on Osphronemus gouramy Physiological Responses. Biosaintifika: Journal of Biology & Biology Education, 8(3), 378-385. </p
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Respons Imunoglobulin-G dan Imunoglobulin-M Mencit yang Diberi Ekstrak Methanol Alga Biru Hijau dan Diinfeksi Dengan Takizoit
No full textToxoplasmosis is an infectious disease caused by Toxoplasma gondii. This disease could severelyaffect humans and animals. Up to now there has been no simple treatment to fight toxoplasmosis. Aprospective alternative treatment to overcome this problem is by increasing immunity of the body using animmunostimulant such as Spirulina platensis. The aims of this research were to observe the potency of S.platensis as an immunostimulant and to find the most potential fraction of S. Platensis that can increasethe responses of IgG and IgM antibodies againts toxoplasma. The responses of these antibodies weremeasured using ELISA method. The isolation of compounds from S. platensis using Preparative ThinLayer Chromatography (PTLC) found three fractions which were a top fraction (I), a middle fraction (II),and a lower fraction (III). Forty-eight mice used in this research were divided into four different groupswith 12 mice in each group and treated differently. The top, middle, and lower fractions of S. platensis wereadministered orally to three groups of mice respectively at dose of 3mg/ml for each mouse while the micein the fourth group were kept as untreated controls. The treatment was conducted for 14 days consecutivelyand on the next day, all mice, including the controls, were challenged with tachizoit. The effect of S.platensisfractions on the responses of IgG and IgM antibodies were then measured at various time intervals, i.e. day0 (before infection) and day 1, 2, and 3 after infection. The results showed that IgG response increased inthe day 0 (2.504 OD) and the day 3 after infection (2.608 OD) while IgM response increased in day 1 afterinfection (2.898 OD). In conclusion, S. platensis was an immunostimulant and the middle fraction (II) of S.Platensis was the most potential fraction to increase immunity againts toxoplasma
Antioxidant Effect of Clorella vulgaris on Wistar Rat Kidney Induced by CCl4: A Histopathological Review
Get PDFKidney is very susceptible to damage by toxicological compounds such as carbon tetrachloride (CCl4). CCl4 produce free radicals, which cause lipid peroxidation and kidney damage and free radical release, which can be prevented by the administration of exogenous antioxidants, such as Chlorella vulgaris.The aim of study was to determine an effect of antioxidant of C. vulgaris on the histopathological features of Wistar rat kidney which is induced by CCl4. Experimental study with completely randomized design. The variable was histopathology features of the kidneys. The doses of C. vulgaris extract were 3 mg, 4 mg, and 5 mg per 100 grams of rat body weight (BW). The administration of C. vulgaris extract was performed within 30 days, while the CCl4 induction (0.25 ml / 100 g BW) was administered orally on the day 9, 12, 16, 19, 23, and 26. Parameters were histopathology features of renal damage, proportion of tubular cell damage, and Bowman's space diameter. The results showed the administration of C. vulgaris extract was able to reduce the impact of damage caused by CCl4 (p<0.05). This was supported by histologic observations, which was showing a decrease of picnotic and vacuolated cells, normal brush border, and decrease of Bowman's space. In conclusion, 5 mg / 100 g BW of C. vulgaris extract can effectively protect the kidney from damage caused by CCl4. The results of this study strongly support further research on immunostimulant content test C. vulgaris and determine the efficient dose for representative animals mammals also in humans.</jats:p
Amylase activity in various digestive organs and blood urea levels of Osphronemus gouramy with Chlorella vulgaris feed supplementation and reared in different system
Get PDFOsphronemus gouramy is a potential Indonesian fish that needs to be supported by good nutritional feed and water quality in a culture system. The research on the effect of combining Chlorella vulgaris (C. vulgaris) supplementation with culture systems of biofloc and non-biofloc in gourami fish on amylase activity and blood urea level has not been conducted. Therefore, this study aims to determine the effect of C. vulgaris supplementation with different culture systems on the amylase activity of various digestive organs and blood urea levels. It obtains the best C. vulgaris supplementation feed combination to increase the amylase activity of various digestive organs and reduce blood urea levels. This research was carried out experimentally with a Factorial Completely Randomized Design consisting of two factors. Furthermore, gourami was fed with C. vulgaris levels of 0, 2, 3, 4, and 5 g kg-1 for 28 days and divided into biofloc and non-biofloc groups of culture systems. The amylase activity was measured at three pH buffers, and blood urea levels were analyzed using a urea kit. The results showed that amylase activity increased when supplemented with C. vulgaris 5 g kg-1 feed using the biofloc system, while blood urea levels decreased. C. vulgaris supplementation and biofloc system are alternative solutions to improving nutrition and water quality
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