177,377 research outputs found
Nuove Prospettive diagnostiche dell'infezione da Pnumocystis carini
No full textNel corso di recenti ricerche sono emersi alcuni dati di rilevante interesse:
1- Evidenziazione di P. carinii in soggetti HIV positivi asintomatici ed HIV negativi esenti da infezioni respiratorie in atto (1). Sono in corso a tal proposito ricerche sistematiche volte a stabilire il significato del riscontro di P. carinii in secrezioni broncoalveolari di pazienti con o senza infezione da HIV, dell'eventuale presenza di stipiti differenti del microrganismo e del loro ruolo nella malattia;
2- Isolamento di P. carinii da cellule mononucleate di pazienti di AIDS con PCP, coltivate su linea A549 di derivazione umana (2). Tale sistema, che ha consentito di porre diagnosi di PCP in una vasta percentuale di casi, potrebbe rappresentare, se i risultati verranno confermati da più ampie casistiche, un approccio alternativo o complementare alla diagnosi dell'infezione da P. carinii.
3- Evidenziazione di alcune componenti ad attività proteasica (MW 100-50 kDa) mediante copolimerizzazione del substrato in gel di poliacrilamide (3). L'impiego di questa metodica ha permesso di visualizzare in Pneumocystis 6 bande ad attività proteasica. In particolare, é stato osservato che la banda 90 kDa viene inibita dall'EDTA, inibitore selettivo delle metalloproteasi. La purificazione di tale banda mediante cromatografia su HPT, suggerisce che questa proteasi é fortemente basica in quanto eluita da cationi bivalenti.
Ulteriori ricerche sono in corso per stabilire il ruolo di questa proteasi nella patogenesi dell'infezione.
Bibliografia essenziale
1- C. Contini, R. Romani, V. Vullo, S. Delia, F. Sorice.
Does laboratory recovery of Pneumocystis carinii always mean clinically significant disease? AIDS, 6, 1558-9, 1992
2- C. Contini, R. Romani, M. Manganaro, F. Sorice, S. Delia.
Tissue culture isolation of Pneumocystis carinii from peripheral blood mononuclear cells of AIDS patients with PCP.
AIDS, 1993, in press.
3- A. P. Massetti, F. Mengoni, C. Contini, G. Sebastiani, F. Folgori, V. Vullo, F. Sorice.
Characterization and partial purification of P. carinii proteases.
VIII International Conference on AIDS, 19-24 July 1992, PoB 3310
Fisiopatologia Generale Respiratoria
No full textLa Patologia e la Fisiopatologia Generale si occupano di studiare i meccanismi patogenetici che sono causa di malattie ed i meccanismi molecolari che sono alla base di ogni patologia d’organo e di ogni patologia sistemica. Trasmettere queste conoscenze è stato fin dalla prima edizione l’obiettivo del manuale di Patologia Generale e Fisiopatologia Generale, che viene ora proseguito dai Professori Mainiero,Misasi e Sorice della Scuola Romana di Patologia. Essi, pertanto, hanno elaborato la sesta edizione del manuale, ampliandolo con le nuove conoscenze in genetica, epigenetica, biologia cellulare e molecolare, fisiologia cellulare, immunologia e patologia molecolare e coinvolgendo numerosi ed autorevoli patologi italiani per trasmettere agli studenti i progressi della scienza in questo campo. (Testo fondato da G.M.Pontieri, I-II Edizione a cura di G.M.Pontieri, III-V Edizione a cura di G.M.Pontieri, M.A.Russo. L.Frati
Il trattamento del carcinoma della mammella.
No full textTreatment of breast carcinoma.
R Fornaro, G Lo Presti, E Belcastro, G Parodi, G Sorice, R Ferraris.
The results of a study on 848 patients with breast carcinoma having maximum diameter less than or equal to 2 cm, that underwent a surgical treatment at the Institute of Special Surgical Pathology B (1980-82) and at the Institute of Surgical Clinic I (1980-89) are presented. The study has the purpose of evaluating, by the analysis of ten years surviving curves, the effectiveness of different types of interventions: Halsted radical mastectomy (98 patients), Patey radical mastectomy (245 patients) and Madden radical mastectomy (151 patients), quadrantectomy (260 patients). The age of patients ranges from 29 years to 92 years (average 56 years). The 68.04% of all tumors have resulted invasive ductal carcinoma (NOS) the 9.08% lobular carcinoma. Of the 848 patients included in the study 698 are alive at the end of the follow-up, with a survival rate, calculated with the actuarial method, equivalent to 63.38%. Results show the survival is independent of the type of surgical treatment (71.9% for quadrantectomy; 70.7% for Halsted radical mastectomy, 68.9% for Patey radical mastectomy; 70.7% for
Madden radical mastectomy)
Appropriate Similarity Measures for Author Cocitation Analysis
No full textWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Oxidized Human Beta2-Glycoprotein I: Its Impact on Innate Immune Cells
No full textBeta2-glycoprotein I (beta 2-GPI), an abundant 50 kDa plasma glycoprotein, is the most common target for antiphospholipid antibodies (aPLs). These autoantibodies are associated with thrombotic events in patients with anti-phospholipid antibody syndrome (APS) and systemic lupus erythematosus (SLE) and are proatherogenic. beta 2-GPI can also stimulate a vigorous adaptive cellular immune response in these patients. Although much is known about beta 2-GPI as a cofactor in autoimmune diseases, crucial information is still lacking to clarify why this abundant self plasma protein is the target of autoimmune responses. Throughout the years, a remarkable number of theories have been proposed to explain how the immune system recognises self. On the basis of a large variety of epidemiological, clinical and experimental evidence, it has been suggested that an unfortunate interplay of genetic susceptibility and environmental factors may play an important role in generating an abnormal immune response. Among the environmental factors, oxidative stress is one of the major events causing protein structural modifications, thus inducing the appearance of neo/cryptic epitopes of beta 2-GPI able to activate the immune system. In particular, oxidized beta 2-GPI is able to induce phenotypic and functional maturation of dendritic cells which represent the link between innate and adaptive immunity. Chronic activation of autoimmune reactions against this self protein modified by oxidative events may contribute to local and systemic inflammation, thus sustaining endothelial dysfunction in patients with APS, SLE and cardiovascular diseases. The role of oxidative stress in beta 2-GPI-mediated immune response is described in the light of our research experience and of relevant literature emerging in the field
Dataset and R script used in "Local knowledge reconstructs historical resource use" published in Frontiers in Ecology and the Environment
No full textData and R code used in: L. Castello, E.G. Martins, M. Sorice, E. Smith, M. Almeida, G.C.C. Bastos, L.G. Cardoso, M. Clauzet, A.P. Dopona, B. Ferreira, M. Haimovici, M. Jorge, J. Mendonça, A.O. Ávila-da-Silva, A.P.O. Roman, M. Ramires, L.M. Villwock, P.F.M. Lopes. (Year) Local knowledge reconstructs historical resource use. Frontiers in Ecology and the Environment
Detection of Pneumocystis carinii in Urinary Samples from a Patient with AIDS Despite Prophylactic Treatment with Aerosolized Pentamidine
No full textThe authors describe a case of AIDS patient prophylaxed with aerosolized pentamidine to prevent PCP who developed dysuria and in whom P. carinii cysts and trophozoites were detected in urinary samples. The Authors hypothesize that pentamidine can favor the entering of P. carinii in systemic circulation and in bladder with clinical consequences
"Closing the R&D Gap, Evaluating the Sources of R&D Spending"
No full textBoth spending and tax policies have been implemented in the United States with the goal of stimulating private sector research and development (R&D). Karier questions whether current R&D policy, especially the research and experimentation tax credit, can contribute to closing the gap between nondefense expenditures on R&D in the United States and such expenditures in other countries, such as Japan and Germany. He also explores possible changes to our current R&D policy to make it more effective.
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