1,721,010 research outputs found
Cloning and Characterization of Mannheimia succiniciproducens MBEL55E Phosphoenolpyruvate Carboxykinase (pckA) Gene
No full textA pckA gene encoding phosphoenolpyruvate carboxykinase (PEPCK) was cloned and sequenced from the succinic acid producing bacterium Mannheimia succiniciproducens MBEL55E. The gene encoded a 538 residue polypeptide with a calculated molecular mass of 58.8 kDa and a calculated pI of 5.03. The deduced amino acid sequence of the M. succiniciprodutens MBEL55E PEPCK was similar to those of all known ATP-dependent PEPCKS.This work was supported by the
Korea Energy Management Corporation (KEMCO) and
by the Bioinfomatix Co
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Evaluating the Potential of OmpC and YiaT for Cobalt Binding Peptide Cell Surface Display and Applications of Adsorbed Cobalt
No full textCobalt, a highly adaptable transition metal found in the d-block of the periodic table, plays a crucial role in the manufacturing of acrylic and ceramic materials. It is a critical electrocatalyst in various industrial processes, including hydroformylation, gasification, and thermal degradation. The extensive use of cobalt may be found in several industries, such as metallurgy, paints, batteries, electroplating, and electronics. The growing demand in the battery industry has led to a significant increase in worldwide cobalt consumption. The increasing demand for cobalt has caused the yearly mining output to rise from 700 to 1200 tonnes between 1995 and 2005, and this upward trend has continued without any decline. As a result, a wide range of recycling methods that involve physical, chemical, physiochemical, and biological processes have been developed to address this urgent need. In this context, nanotechnology has significantly transformed the sector by demonstrating the production of nanocrystalline cobalt oxide materials by various methods, particularly the microemulsion technique, which is known for precisely adjusting the characteristics of nanoparticles. Although cobalt nanoparticles obtained from botanical and microbial sources are stable, there has been limited focus on synthesizing cobalt oxide nanoparticles using microbes. This work explores the utilisation of metal binding peptides (MBP) in the microbial cell surface display (CSD) technique for cobalt recovery. The inquiry reveals the exhibition of cobalt MBP (Metal Binding Protein) on E. coli, a crucial milestone in developing a sustainable method for extracting cobalt from diverse sources. Research efforts have explored innovative approaches for displaying cells on the surface, specifically focusing on utilising the outer membrane proteins of Escherichia coli, namely OmpC and YiaT. These proteins act as structural frameworks for binding peptides that can capture heavy metals. This leads to the creation of a new type of biosorbent, which involves using cells that produce peptides capable of binding to cobalt. This allows for the extraction of cobalt from polluted water sources. The study thoroughly assesses four cobalt binding peptide fragments derived from CP1 and CP2, expressed on the cell surface. The fusion protein-based method using OmpC as the anchor shows promising rates of cobalt recovery. This is highlighted by the excellent performance of specific peptide fragments such as CF4 and CF2, evident in different medium compositions. Furthermore, the examination explores the use of thermal treatments to reveal the structure and characteristics of cobalt oxide nanoparticles obtained from cobalt that have been adsorbed by biological means. This study highlights the potential of these nanoparticles in several applications, including anticancer research, dye photocatalytic degradation, and drug decomposition. This research introduces a new approach to using microorganisms to produce metal nanoparticles and remediate wastewater. It achieves this by utilizing synthetic peptides that are produced by the bacteria. The results provide insight into the effectiveness of peptide-cobalt interactions, their ability to selectively bind, and the diverse possibilities of cobalt-binding peptides and nanoparticles in tackling industrial and environmental issues. These discoveries enhance our understanding of how peptides interact with metals, leading to significant advancements in several fields.Docto
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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